B6.129S1(Cg)-Zfpm2^tm1Sho/EiJ

Stock number: 005646
Product name: Fog2-

Description

Zfpm2 (formerly Fog2) knock-out embryos die at mid-gestation and exhibit cardiac and gonadal differentiation defects.

Allele

Symbol: Zfpm2^tm1Sho
Name: targeted mutation 1, Stuart Orkin
MGI accession ID: MGI:2386183
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Zfpm2
Marker name: zinc finger protein, multitype 2
Chromosome: 15
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Molecular note: The gene was disrupted by replacement of sequences encoding all of the zinc finger motifs with a floxed neomycin-cytidine deaminase cassette. The floxed cassette was removed by crossing mutant animals to a cre deleter strain. Homozygous mutant embryos were detected by Southern blot analysis up to day E15.5. RNase protection assay showed an absence of Zfpm2 mRNA in hearts of E12.5 homozygous mutant embryos.