These Fcer1a knock-out mice are resistant to IgE induced anaphylaxis and are susceptible to IgG1-dependent anaphylaxis treatments. They are suitable for use in studies of anaphylaxis, and immunological response to allergens.
Jean-Pierre Kinet, Beth Israel Deaconess Medical Center
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Cultured bone marrow-derived mast cells from homozygotes, that have been activated with interleukin-3, do not bind to monomeric IgE as analyzed by flow cytometry. Mice homozygous for the mutant allele are resistant to IgE induced passive cutaneous and systemic anaphylaxis and are more susceptible to IgG1-dependent passive and active systemic anaphylaxis treatments. This mutant mouse strain may be useful in studies of anaphylaxis, and immunological response to allergens.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 4, which encodes an immunoglobulin domain. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to BALB/cJ mice, and then backcrossed to the same for 20 generations.
|Allele Name||targeted mutation 1, Jean-Pierre Kinet|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Fc epsilon RI alpha chain-; FcepsilonR1alpha-; FcepsilonRI-; Fcer1a-; Fcer1atm1Rav; FcerIa-|
|Gene Symbol and Name||Fcer1a, Fc receptor, IgE, high affinity I, alpha polypeptide|
|Strain of Origin||129|
|General Note||ES cell line = D3 (129S2/SvPas) or E14TG2a (129P2/OlaHsd).|
|Molecular Note||Exon 4, encoding an immunoglobulin domain, was disrupted by the insertion of a neomycin selection cassette. The absence of a functional encoded protein on the cell surface was determined by fluorescence activated cell sorting analysis of bone marrow mast cells obtained from homozygous mutant mice.|
|Mutations Made By|| |
Devin Turner, Beth Israel Deaconess Medical Center
When maintaining a live colony, these mice are bred as homozygotes.
When using the FcεRI- mouse strain in a publication, please cite the originating article(s) and include JAX stock #005629 in your Materials and Methods section.