These Fcer1a knock-out mice are resistant to IgE induced anaphylaxis and are susceptible to IgG1-dependent anaphylaxis treatments. They are suitable for use in studies of anaphylaxis, and immunological response to allergens.
Jean-Pierre Kinet, Beth Israel Deaconess Medical Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Fcer1a | Fc receptor, IgE, high affinity I, alpha polypeptide |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Cultured bone marrow-derived mast cells from homozygotes, that have been activated with interleukin-3, do not bind to monomeric IgE as analyzed by flow cytometry. Mice homozygous for the mutant allele are resistant to IgE induced passive cutaneous and systemic anaphylaxis and are more susceptible to IgG1-dependent passive and active systemic anaphylaxis treatments. This mutant mouse strain may be useful in studies of anaphylaxis, and immunological response to allergens.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 4, which encodes an immunoglobulin domain. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to BALB/cJ mice, and then backcrossed to the same for 20 generations.
Allele Name | targeted mutation 1, Jean-Pierre Kinet |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Fc epsilon RI alpha chain-; FcepsilonR1alpha-; FcepsilonRI-; Fcer1a-; Fcer1atm1Rav; FcerIa- |
Gene Symbol and Name | Fcer1a, Fc receptor, IgE, high affinity I, alpha polypeptide |
Gene Synonym(s) | |
Strain of Origin | 129 |
Chromosome | 1 |
General Note | ES cell line = D3 (129S2/SvPas) or E14TG2a (129P2/OlaHsd). |
Molecular Note | Exon 4, encoding an immunoglobulin domain, was disrupted by the insertion of a neomycin selection cassette. The absence of a functional encoded protein on the cell surface was determined by fluorescence activated cell sorting analysis of bone marrow mast cells obtained from homozygous mutant mice. |
Mutations Made By | Devin Turner, Beth Israel Deaconess Medical Center |
When maintaining a live colony, these mice are bred as homozygotes.
When using the FcεRI- mouse strain in a publication, please cite the originating article(s) and include JAX stock #005629 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Fcer1a<tm1Knt> |
Frozen Mouse Embryo | C.129S2-Fcer1a<tm1Knt>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C.129S2-Fcer1a<tm1Knt>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C.129S2-Fcer1a<tm1Knt>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | C.129S2-Fcer1a<tm1Knt>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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