C.129S2-Fcer1a^tm1Knt/J

Stock number: 005629
Product name: FcεRI-
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These Fcer1a knock-out mice are resistant to IgE induced anaphylaxis and are susceptible to IgG1-dependent anaphylaxis treatments. They are suitable for use in studies of anaphylaxis, and immunological response to allergens.

Detailed description

Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Cultured bone marrow-derived mast cells from homozygotes, that have been activated with interleukin-3, do not bind to monomeric IgE as analyzed by flow cytometry. Mice homozygous for the mutant allele are resistant to IgE induced passive cutaneous and systemic anaphylaxis and are more susceptible to IgG1-dependent passive and active systemic anaphylaxis treatments. This mutant mouse strain may be useful in studies of anaphylaxis, and immunological response to allergens.

Development

A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 4, which encodes an immunoglobulin domain. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to BALB/cJ mice, and then backcrossed to the same for 20 generations.

Allele

Symbol: Fcer1a^tm1Knt
Name: targeted mutation 1, Jean-Pierre Kinet
MGI accession ID: MGI:2383982
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Fcer1a
Marker name: Fc receptor, IgE, high affinity I, alpha polypeptide
Chromosome: 1
Strain of origin: 129
Molecular note: Exon 4, encoding an immunoglobulin domain, was disrupted by the insertion of a neomycin selection cassette. The absence of a functional encoded protein on the cell surface was determined by fluorescence activated cell sorting analysis of bone marrow mast cells obtained from homozygous mutant mice.
General note: ES cell line = D3 (129S2/SvPas) or E14TG2a (129P2/OlaHsd).