FVB-Tg(Pcp2-tTA)3Horr/J

Stock number: 005625
Product name: SCA1
Research areas: Neurobiology Research, Research Tools

Description

The transgene in these SCA1 mice contains the tetracycline transactivator (tTA) under control of the Purkinje cell protein 2 ( Pcp2 promoter.

Detailed description

Mice that are homozygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Cerebellar Purkinje cell-specific tTA expression has been confirmed using PCR. Constitutive expression of tTA in Purkinje cells makes this strain suitable for creating bitransgenic mice that can, by withdrawing tetracycline (or its derivative doxycycline), inducibly express a gene of interest in Purkinje cells when the gene of interest is under the direction of a tetracycline-responsive element (TRE; tetO).

Development

A transgenic construct using the Purkinje cell-specific murine Pcp2 regulatory region to direct expression of the tetracycline-regulated transactivator, tTA, was injected into FVB/N embryos. These embryos were implanted into pseudopregnant FVB/N females. Founder animals were backcrossed to FVB/N for five generations and were maintained by intercross.

Allele

Symbol: Tg(Pcp2-tTA)3Horr
Name: transgene insertion 3, Harry T Orr
MGI accession ID: MGI:3580157
Generation method: Transgenic
Attributes: Transactivator
Marker symbol: Tg(Pcp2-tTA)3Horr
Marker name: transgene insertion 3, Harry T Orr
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: tTA,tetracycline-controlled transactivator,E. coli
Site of expression: Expresses tTA in cerebellar Purkinje cells.
Molecular note: This transgene expresses the tetracycline transactivator (tTA) under control of the 5' sequences, including the first exon, the first intron and part of the second exon, of the Purkinje-cell specific mouse Purkinje cell protein 2 gene.
General note: Transgene mRNA levels in cerebella of mice of nine independent transgenic lines were assayed by Northern blot analysis of cerebellar extracts. The highest-expressing line was selected to cross with mice bearing a TetO-driven transgene.