Although the Lepob mutation has been selected against, it may still be segregating in this strain.
Subsequently, likely as a consequence of infection, homozygotes develop a small white spot and atypical growth of blood vessels on the cornea of each eye, although occasionally only one eye is affected.
The whe mutation arose spontaneously in the strain B6(V)-Lepob/J in 2003. Although the strain was maintained by sibling mating, phenotypically non-obese breeders were selected in order to breed the Lepob mutation out of the line. Nevertheless, obese mice were still present in this strain at generation F4. In 2006 C57BL/6J females and whe homozygous males at generation F5 were used for in vitro fertilization to generate embryos for cryopreservation.
|Allele Name||white eyes|
|Gene Symbol and Name||Limk2, LIM motif-containing protein kinase 2|
|Strain of Origin||B6.Cg-Lepob/J|
|Molecular Note||A spontaneous mutation results in a C-to-A point mutation in the first base of intron 12. This eliminates the exon 12 splice donor site by changing it from G-GT to G-TT.|