B6.Cg-Lep^ob Limk2^whe/GrsrJ

Stock number: 005624
Product name: white eyes
Research areas: Developmental Biology Research, Sensorineural Research

Description

These mice carry a spontaneous mutation of the Limk2 gene characterized by a white spot on the eye.

Detailed description

Subsequently, likely as a consequence of infection, homozygotes develop a small white spot and atypical growth of blood vessels on the cornea of each eye, although occasionally only one eye is affected.

Development

The whe mutation arose spontaneously in the strain B6(V)-Lep^ob/J in 2003. Although the strain was maintained by sibling mating, phenotypically non-obese breeders were selected in order to breed the Lep^ob mutation out of the line. Nevertheless, obese mice were still present in this strain at generation F4. In 2006 C57BL/6J females and whe homozygous males at generation F5 were used for in vitro fertilization to generate embryos for cryopreservation.

Allele

Symbol: Limk2^whe
Name: white eyes
MGI accession ID: MGI:3612836
Generation method: Spontaneous
Marker symbol: Limk2
Marker name: LIM motif-containing protein kinase 2
Chromosome: 11
Strain of origin: B6.Cg-Lep^ob/J
Molecular note: A spontaneous mutation results in a C-to-A point mutation in the first base of intron 12. This eliminates the exon 12 splice donor site by changing it from G-GT to G-TT.