JAX
Mouse Strain Datasheet - 005622
B6.Cg-Shhtm1(EGFP/cre)Cjt/J
Also Known As: Shhgfpcre
Fluorescence in these mice, consistent with the endogenous Shh locus, is detected in the distal posterior region of the limb buds of embryos aged embryonic day 10 to 12. Mice homozygous for the mutation develop a limited limb skeleton and lack digit 2. This mutant mouse strain may be useful in studies of limb patterning and development.
Donating Investigator
Clifford J Tabin, Harvard Medical School
Genetic overview
| Genetic Background | Generation |
|---|---|
|
N7F10
(18-JUL-16) |
Shhtm1(EGFP/cre)Cjt
| Allele Type | Gene Symbol | Gene Name |
|---|---|---|
| Targeted (Null/Knockout, Recombinase-expressing) | Shh | sonic hedgehog |
Base Price
Starting at:
| $263.00 Domestic price for female |
| $337.00 Domestic price for breeder pair |
Detailed Description
This strain expresses a fusion product involving Enhanced Green Fluorescent Protein (EGFP) and Cre recombinase from the endogenous Shh locus. EGFP and cre expression are consistent with the endogenous gene. Fluorescence is detected in the distal posterior region of the limb buds of embryos aged embryonic day 10 to 12 and colocalizes with the endogenous gene product (mRNA). The donating investigator reports that it is not uncommon for a mosaic expression pattern to be exhibited when the allele is inherited through the female germline. It is recommended that this allele be passed through the male germline when conducting experiments involving cre-induced recombination. Mice homozygous for the mutation develop a limited limb skeleton and lack digit 2. Homozygous mice are not viable or fertile. Heterozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of limb patterning and development.
Development
A targeting vector containing a fusion product involving Enhanced Green Fluorescent Protein (EGFP) and Cre recombinase was inserted at the ATG of Shh and disrupted the sequence encoding the first 12 amino acids. The construct was electroporated into 129S6/SvEv-derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and the donating investigator reported that they were then backcrossed to the same for at least 5 generations.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
Expression Data
| Expressed Gene | GFP, Green Fluorescent Protein, |
|---|---|
| Expressed Gene | cre, cre recombinase, bacteriophage P1 |
| Site of Expression | Distal posterior region of the limb buds of embryos aged embryonic day 10 to 12; also a Cre strain. |
Control Suggestions
- Wild-type from the colony
- 000664 C57BL/6J
Additional Information
Selected References
- Harfe BD; Scherz PJ; Nissim S; Tian H; McMahon AP; Tabin CJ. 2004. Evidence for an expansion-based temporal Shh gradient in specifying vertebrate digit identities. Cell 118(4):517-28. PubMed: 15315763MGI: J:92504
Shhtm1(EGFP/cre)Cjt
Allele Symbol: Shhtm1(EGFP/cre)Cjt
| Allele Name | targeted mutation 1, Clifford J Tabin |
|---|---|
| Allele Type | Targeted (Null/Knockout, Recombinase-expressing) |
| Allele Synonym(s) | Shh-GFP-Cre; Shh-cre; ShhCreEGFP; ShhGFP-Cre; Shhcre/Gfp; Shhgfpcre; Shhgfp-Cre; Shhgfpcre |
| Gene Symbol and Name | Shh, sonic hedgehog |
| Gene Synonym(s) | Dsh; HHG1; HLP3; HPE3; Hhg1; Hhg1; Hx; Hx; Hxl3; Hxl3; M100081; MCOPCB5; SMMCI; TPT; TPTPS; hedgehog gene 1; hemimelic extra toes; hemimelic extratoes like 3; short digits |
| Expressed Gene | GFP, Green Fluorescent Protein, |
| Expressed Gene | cre, cre recombinase, bacteriophage P1 |
| Site of Expression | Distal posterior region of the limb buds of embryos aged embryonic day 10 to 12; also a Cre strain. |
| Strain of Origin | Not Specified |
| Chromosome | 5 |
| Molecular Note | A cassette containing an in frame fusion between GFP and cre was inserted at the ATG. Upon recombination, the first 35 base pairs after the ATG were deleted. Fluorescence was detected in the distal posterior region of E10-E12 embryos. |
| Mutations Made By | Clifford Tabin, Harvard Medical School |
Disease Terms
Research Areas By Genotype
This mouse can be used to support research in many areas including:
- Developmental Biology Research
- Embryonic Lethality (Homozygous)
- Limb Patterning Defects
- Skeletal Defects
- Research Tools
- Cre-lox System
- Cre Recombinase Expression
- Cre Recombinase Expression: Germline/Embryonic Expression
- Developmental Biology Research
- Cre-lox System
- Fluorescent Proteins
- Genetics Research
- Mutagenesis and Transgenesis
- Mutagenesis and Transgenesis: Cre-lox System
- Cre-lox System
Genotype: GFP related
- Research Tools
- Fluorescent Proteins
Genotype: cre related
- Research Tools
- Cre-lox System
- Genetics Research
- Mutagenesis and Transgenesis
- Mutagenesis and Transgenesis: Cre-lox System
Mammalian Phenotype Terms by Genotype
The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain
Genotype: Shhtm1(EGFP/cre)Cjt/Shhtm1(EGFP/cre)Cjt
Not Specified
cardiovascular system phenotype
- abnormal atrium myocardium morphology
- mutants show reduced muscular development in dorsal wall of atria (MGI Ref ID J:204743)
- abnormal heart left atrium morphology
- (MGI Ref ID J:204743)
- abnormal pulmonary artery morphology
- pulmonary arteries connect to aorta instead of the ouflow tract (MGI Ref ID J:204743)
- abnormal vascular smooth muscle morphology
- reduction in vascular and airway smooth muscle derived from Isl1- and Gli1-positive cardiopulmonary mesoderm progenitors is detected (MGI Ref ID J:204743)
- abnormal vasculogenesis
- embryos have a disorganized vascular endothelial plexus along anterior foregut which does not connect to heart inflow and outflow tracts (MGI Ref ID J:204743)
- absent pulmonary vein
- pulmonary veins from left atrium to lung are absent (MGI Ref ID J:204743)
muscle phenotype
- abnormal vascular smooth muscle morphology
- reduction in vascular and airway smooth muscle derived from Isl1- and Gli1-positive cardiopulmonary mesoderm progenitors is detected (MGI Ref ID J:204743)
limbs/digits/tail phenotype
- abnormal limb bone morphology
- a limited skeletal structure consisting of the humerus, radius and single digit 1 is seen (MGI Ref ID J:92504)
skeleton phenotype
- abnormal limb bone morphology
- a limited skeletal structure consisting of the humerus, radius and single digit 1 is seen (MGI Ref ID J:92504)
respiratory system phenotype
- abnormal respiratory conducting tube morphology
- airway smooth muscle derived from Isl1- and Gli1-positive cardiopulmonary mesoderm progenitors is significantly reduced; airway smooth muscle development is reduced (MGI Ref ID J:204743)
References
- Harfe BD; Scherz PJ; Nissim S; Tian H; McMahon AP; Tabin CJ. 2004. Evidence for an expansion-based temporal Shh gradient in specifying vertebrate digit identities. Cell 118(4):517-28. PubMed: 15315763MGI: J:92504
Additional - Shhtm1(EGFP/cre)Cjt related
- Achim K; Peltopuro P; Lahti L; Li J; Salminen M; Partanen J. 2012. Distinct developmental origins and regulatory mechanisms for GABAergic neurons associated with dopaminergic nuclei in the ventral mesodiencephalic region. Development 139(13):2360-70. PubMed: 22627282MGI: J:185533
- Ahn Y; Sanderson BW; Klein OD; Krumlauf R. 2010. Inhibition of Wnt signaling by Wise (Sostdc1) and negative feedback from Shh controls tooth number and patterning. Development 137(19):3221-31. PubMed: 20724449MGI: J:168361
- Anderegg A; Lin HP; Chen JA; Caronia-Brown G; Cherepanova N; Yun B; Joksimovic M; Rock J; Harfe BD; Johnson R; Awatramani R. 2013. An Lmx1b-miR135a2 regulatory circuit modulates Wnt1/Wnt signaling and determines the size of the midbrain dopaminergic progenitor pool. PLoS Genet 9(12):e1003973. PubMed: 24348261MGI: J:207519
- Aoto K; Trainor PA. 2015. Co-ordinated brain and craniofacial development depend upon Patched1/XIAP regulation of cell survival. Hum Mol Genet 24(3):698-713. PubMed: 25292199MGI: J:218093
- Bell SM; Zhang L; Mendell A; Xu Y; Haitchi HM; Lessard JL; Whitsett JA. 2011. Kruppel-like factor 5 is required for formation and differentiation of the bladder urothelium. Dev Biol 358(1):79-90. PubMed: 21803035MGI: J:176604
- Bell SM; Zhang L; Xu Y; Besnard V; Wert SE; Shroyer N; Whitsett JA. 2013. Kruppel-like factor 5 controls villus formation and initiation of cytodifferentiation in the embryonic intestinal epithelium. Dev Biol 375(2):128-39. PubMed: 23266329MGI: J:194289
- Belle M; Godefroy D; Dominici C; Heitz-Marchaland C; Zelina P; Hellal F; Bradke F; Chedotal A. 2014. A simple method for 3D analysis of immunolabeled axonal tracts in a transparent nervous system. Cell Rep 9(4):1191-201. PubMed: 25456121MGI: J:218756
- Bodea GO; Spille JH; Abe P; Andersson AS; Acker-Palmer A; Stumm R; Kubitscheck U; Blaess S. 2014. Reelin and CXCL12 regulate distinct migratory behaviors during the development of the dopaminergic system. Development 141(3):661-73. PubMed: 24449842MGI: J:208337
- Boucherat O; Landry-Truchon K; Berube-Simard FA; Houde N; Beuret L; Lezmi G; Foulkes WD; Delacourt C; Charron J; Jeannotte L. 2015. Epithelial inactivation of Yy1 abrogates lung branching morphogenesis. Development 142(17):2981-95. PubMed: 26329601MGI: J:239777
- Boucherat O; Nadeau V; Berube-Simard FA; Charron J; Jeannotte L. 2014. Crucial requirement of ERK/MAPK signaling in respiratory tract development. Development 141(16):3197-211. PubMed: 25100655MGI: J:214083
- Chamberlain CE; Jeong J; Guo C; Allen BL; McMahon AP. 2008. Notochord-derived Shh concentrates in close association with the apically positioned basal body in neural target cells and forms a dynamic gradient during neural patterning. Development 135(6):1097-106. PubMed: 18272593MGI: J:132152
- Chang DR; Martinez Alanis D; Miller RK; Ji H; Akiyama H; McCrea PD; Chen J. 2013. Lung epithelial branching program antagonizes alveolar differentiation. Proc Natl Acad Sci U S A 110(45):18042-51. PubMed: 24058167MGI: J:202949
- Chang H; Smallwood PM; Williams J; Nathans J. 2016. The spatio-temporal domains of Frizzled6 action in planar polarity control of hair follicle orientation. Dev Biol 409(1):181-93. PubMed: 26517967MGI: J:228792
- Choi KS; Harfe BD. 2011. Hedgehog signaling is required for formation of the notochord sheath and patterning of nuclei pulposi within the intervertebral discs. Proc Natl Acad Sci U S A 108(23):9484-9. PubMed: 21606373MGI: J:173333
- Cocas LA; Fernandez G; Barch M; Doll J; Zamora Diaz I; Pleasure SJ. 2016. Cell Type-Specific Circuit Mapping Reveals the Presynaptic Connectivity of Developing Cortical Circuits. J Neurosci 36(11):3378-90. PubMed: 26985044MGI: J:231657
- Croyle MJ; Lehman JM; O'Connor AK; Wong SY; Malarkey EB; Iribarne D; Dowdle WE; Schoeb TR; Verney ZM; Athar M; Michaud EJ; Reiter JF; Yoder BK. 2011. Role of epidermal primary cilia in the homeostasis of skin and hair follicles. Development 138(9):1675-85. PubMed: 21429982MGI: J:171221
- Desai TJ; Brownfield DG; Krasnow MA. 2014. Alveolar progenitor and stem cells in lung development, renewal and cancer. Nature 507(7491):190-4. PubMed: 24499815MGI: J:207468
- Dixit R; Ai X; Fine A. 2013. Derivation of lung mesenchymal lineages from the fetal mesothelium requires hedgehog signaling for mesothelial cell entry. Development 140(21):4398-406. PubMed: 24130328MGI: J:204973
- Domyan ET; Branchfield K; Gibson DA; Naiche LA; Lewandoski M; Tessier-Lavigne M; Ma L; Sun X. 2013. Roundabout receptors are critical for foregut separation from the body wall. Dev Cell 24(1):52-63. PubMed: 23328398MGI: J:193400
- Domyan ET; Ferretti E; Throckmorton K; Mishina Y; Nicolis SK; Sun X. 2011. Signaling through BMP receptors promotes respiratory identity in the foregut via repression of Sox2. Development 138(5):971-81. PubMed: 21303850MGI: J:169134
- Fabian SL; Penchev RR; St-Jacques B; Rao AN; Sipila P; West KA; McMahon AP; Humphreys BD. 2012. Hedgehog-Gli Pathway Activation during Kidney Fibrosis. Am J Pathol 180(4):1441-53. PubMed: 22342522MGI: J:182155
- Fantauzzo KA; Kurban M; Levy B; Christiano AM. 2012. Trps1 and its target gene Sox9 regulate epithelial proliferation in the developing hair follicle and are associated with hypertrichosis. PLoS Genet 8(11):e1003002. PubMed: 23133399MGI: J:194118
- Fausett SR; Brunet LJ; Klingensmith J. 2014. BMP antagonism by Noggin is required in presumptive notochord cells for mammalian foregut morphogenesis. Dev Biol 391(1):111-24. PubMed: 24631216MGI: J:213659
- Flandin P; Kimura S; Rubenstein JL. 2010. The progenitor zone of the ventral medial ganglionic eminence requires Nkx2-1 to generate most of the globus pallidus but few neocortical interneurons. J Neurosci 30(8):2812-23. PubMed: 20181579MGI: J:157830
- Galvis LA; Holik AZ; Short KM; Pasquet J; Lun AT; Blewitt ME; Smyth IM; Ritchie ME; Asselin-Labat ML. 2015. Repression of Igf1 expression by Ezh2 prevents basal cell differentiation in the developing lung. Development 142(8):1458-69. PubMed: 25790853MGI: J:220467
- Gandhi D; Molotkov A; Batourina E; Schneider K; Dan H; Reiley M; Laufer E; Metzger D; Liang F; Liao Y; Sun TT; Aronow B; Rosen R; Mauney J; Adam R; Rosselot C; Van Batavia J; McMahon A; McMahon J; Guo JJ; Mendelsohn C. 2013. Retinoid signaling in progenitors controls specification and regeneration of the urothelium. Dev Cell 26(5):469-82. PubMed: 23993789MGI: J:204539
- Garriock RJ; Chalamalasetty RB; Kennedy MW; Canizales LC; Lewandoski M; Yamaguchi TP. 2015. Lineage tracing of neuromesodermal progenitors reveals novel Wnt-dependent roles in trunk progenitor cell maintenance and differentiation. Development 142(9):1628-38. PubMed: 25922526MGI: J:221408
- Genetic Resource Science at The Jackson Laboratory. 2012. Expression/Specificity Patterns of Cre Alleles, 2012 Direct Data Submission from Genetic Resource Science :. MGI: J:184579
- Glasco DM; Pike W; Qu Y; Reustle L; Misra K; Di Bonito M; Studer M; Fritzsch B; Goffinet AM; Tissir F; Chandrasekhar A. 2016. The atypical cadherin Celsr1 functions non-cell autonomously to block rostral migration of facial branchiomotor neurons in mice. Dev Biol 417(1):40-9. PubMed: 27395006MGI: J:235628
- Glotzer DJ; Zelzer E; Olsen BR. 2008. Impaired skin and hair follicle development in Runx2 deficient mice. Dev Biol 315(2):459-73. PubMed: 18262513MGI: J:132568
- Goss AM; Tian Y; Tsukiyama T; Cohen ED; Zhou D; Lu MM; Yamaguchi TP; Morrisey EE. 2009. Wnt2/2b and beta-catenin signaling are necessary and sufficient to specify lung progenitors in the foregut. Dev Cell 17(2):290-8. PubMed: 19686689MGI: J:153098
- Gredler ML; Seifert AW; Cohn MJ. 2015. Tissue-specific roles of Fgfr2 in development of the external genitalia. Development 142(12):2203-12. PubMed: 26081573MGI: J:224075
- Greif DM; Kumar M; Lighthouse JK; Hum J; An A; Ding L; Red-Horse K; Espinoza FH; Olson L; Offermanns S; Krasnow MA. 2012. Radial construction of an arterial wall. Dev Cell 23(3):482-93. PubMed: 22975322MGI: J:189012
- Gritli-Linde A; Hallberg K; Harfe BD; Reyahi A; Kannius-Janson M; Nilsson J; Cobourne MT; Sharpe PT; McMahon AP; Linde A. 2007. Abnormal hair development and apparent follicular transformation to mammary gland in the absence of hedgehog signaling. Dev Cell 12(1):99-112. PubMed: 17199044MGI: J:117334
- Guo C; Balsara ZR; Hill WG; Li X. 2017. Stage- and subunit-specific functions of polycomb repressive complex 2 in bladder urothelial formation and regeneration. Development 144(3):400-408. PubMed: 28049658MGI: J:239161
- Haara O; Fujimori S; Schmidt-Ullrich R; Hartmann C; Thesleff I; Mikkola ML. 2011. Ectodysplasin and Wnt pathways are required for salivary gland branching morphogenesis. Development 138(13):2681-91. PubMed: 21652647MGI: J:173573
- Harfe BD; McManus MT; Mansfield JH; Hornstein E; Tabin CJ. 2005. The RNaseIII enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limb. Proc Natl Acad Sci U S A 102(31):10898-903. PubMed: 16040801MGI: J:100475
- Harjunmaa E; Kallonen A; Voutilainen M; Hamalainen K; Mikkola ML; Jernvall J. 2012. On the difficulty of increasing dental complexity. Nature 483(7389):324-7. PubMed: 22398444MGI: J:182586
- Harris KS; Zhang Z; McManus MT; Harfe BD; Sun X. 2006. Dicer function is essential for lung epithelium morphogenesis. Proc Natl Acad Sci U S A 103(7):2208-13. PubMed: 16452165MGI: J:106072
- Harwell CC; Parker PR; Gee SM; Okada A; McConnell SK; Kreitzer AC; Kriegstein AR. 2012. Sonic hedgehog expression in corticofugal projection neurons directs cortical microcircuit formation. Neuron 73(6):1116-26. PubMed: 22445340MGI: J:183457
- Hayes L; Ralls S; Wang H; Ahn S. 2013. Duration of Shh signaling contributes to mDA neuron diversity. Dev Biol 374(1):115-26. PubMed: 23201023MGI: J:193109
- Herriges JC; Verheyden JM; Zhang Z; Sui P; Zhang Y; Anderson MJ; Swing DA; Zhang Y; Lewandoski M; Sun X. 2015. FGF-Regulated ETV Transcription Factors Control FGF-SHH Feedback Loop in Lung Branching. Dev Cell 35(3):322-32. PubMed: 26555052MGI: J:240020
- Hsu YC; Li L; Fuchs E. 2014. Transit-amplifying cells orchestrate stem cell activity and tissue regeneration. Cell 157(4):935-49. PubMed: 24813615MGI: J:214395
- Huang YC; Chen F; Li X. 2016. Clarification of mammalian cloacal morphogenesis using high-resolution episcopic microscopy. Dev Biol 409(1):106-13. PubMed: 26485363MGI: J:240373
- Ishida K; Murofushi M; Nakao K; Morita R; Ogawa M; Tsuji T. 2011. The regulation of tooth morphogenesis is associated with epithelial cell proliferation and the expression of Sonic hedgehog through epithelial-mesenchymal interactions. Biochem Biophys Res Commun 405(3):455-61. PubMed: 21255557MGI: J:170606
- Jiang M; Ku WY; Fu J; Offermanns S; Hsu W; Que J. 2013. Gpr177 regulates pulmonary vasculature development. Development 140(17):3589-94. PubMed: 23884445MGI: J:199291
- Joksimovic M; Yun BA; Kittappa R; Anderegg AM; Chang WW; Taketo MM; McKay RD; Awatramani RB. 2009. Wnt antagonism of Shh facilitates midbrain floor plate neurogenesis. Nat Neurosci 12(2):125-31. PubMed: 19122665MGI: J:146200
- Joo JH; Taxter TJ; Munguba GC; Kim YH; Dhaduvai K; Dunn NW; Degan WJ; Oh SP; Sugrue SP. 2010. Pinin modulates expression of an intestinal homeobox gene, Cdx2, and plays an essential role for small intestinal morphogenesis. Dev Biol 345(2):191-203. PubMed: 20637749MGI: J:164807
- Juuri E; Jussila M; Seidel K; Holmes S; Wu P; Richman J; Heikinheimo K; Chuong CM; Arnold K; Hochedlinger K; Klein O; Michon F; Thesleff I. 2013. Sox2 marks epithelial competence to generate teeth in mammals and reptiles. Development 140(7):1424-32. PubMed: 23462476MGI: J:194917
- Juuri E; Saito K; Ahtiainen L; Seidel K; Tummers M; Hochedlinger K; Klein OD; Thesleff I; Michon F. 2012. Sox2+ Stem Cells Contribute to All Epithelial Lineages of the Tooth via Sfrp5+ Progenitors. Dev Cell 23(2):317-28. PubMed: 22819339MGI: J:187606
- Kadzik RS; Cohen ED; Morley MP; Stewart KM; Lu MM; Morrisey EE. 2014. Wnt ligand/Frizzled 2 receptor signaling regulates tube shape and branch-point formation in the lung through control of epithelial cell shape. Proc Natl Acad Sci U S A 111(34):12444-9. PubMed: 25114215MGI: J:213915
- Kasberg AD; Brunskill EW; Steven Potter S. 2013. SP8 regulates signaling centers during craniofacial development. Dev Biol 381(2):312-23. PubMed: 23872235MGI: J:200761
- Kim BM; Mao J; Taketo MM; Shivdasani RA. 2007. Phases of canonical Wnt signaling during the development of mouse intestinal epithelium. Gastroenterology 133(2):529-38. PubMed: 17681174MGI: J:128278
- Kim BM; Miletich I; Mao J; McMahon AP; Sharpe PA; Shivdasani RA. 2007. Independent functions and mechanisms for homeobox gene Barx1 in patterning mouse stomach and spleen. Development 134(20):3603-13. PubMed: 17855428MGI: J:128378
- Kim TH; Kim BM; Mao J; Rowan S; Shivdasani RA. 2011. Endodermal Hedgehog signals modulate Notch pathway activity in the developing digestive tract mesenchyme. Development 138(15):3225-33. PubMed: 21750033MGI: J:180905
- King P; Paul A; Laufer E. 2009. Shh signaling regulates adrenocortical development and identifies progenitors of steroidogenic lineages. Proc Natl Acad Sci U S A 106(50):21185-90. PubMed: 19955443MGI: J:155826
- Kittappa R; Chang WW; Awatramani RB; McKay RD. 2007. The foxa2 gene controls the birth and spontaneous degeneration of dopamine neurons in old age. PLoS Biol 5(12):e325. PubMed: 18076286MGI: J:130846
- Kur E; Mecklenburg N; Cabrera RM; Willnow TE; Hammes A. 2014. LRP2 mediates folate uptake in the developing neural tube. J Cell Sci 127(Pt 10):2261-8. PubMed: 24639464MGI: J:214694
- Lahti L; Saarimaki-Vire J; Rita H; Partanen J. 2011. FGF signaling gradient maintains symmetrical proliferative divisions of midbrain neuronal progenitors. Dev Biol 349(2):270-82. PubMed: 21074523MGI: J:168023
- Li G; Fang L; Fernandez G; Pleasure SJ. 2013. The ventral hippocampus is the embryonic origin for adult neural stem cells in the dentate gyrus. Neuron 78(4):658-72. PubMed: 23643936MGI: J:201555
- Li S; Morley M; Lu M; Zhou S; Stewart K; French CA; Tucker HO; Fisher SE; Morrisey EE. 2016. Foxp transcription factors suppress a non-pulmonary gene expression program to permit proper lung development. Dev Biol 416(2):338-46. PubMed: 27341756MGI: J:234990
- Li S; Wang Y; Zhang Y; Lu MM; Demayo FJ; Dekker JD; Tucker PW; Morrisey EE. 2012. Foxp1/4 control epithelial cell fate during lung development and regeneration through regulation of anterior gradient 2. Development 139(14):2500-9. PubMed: 22675208MGI: J:185603
- Li X; Liu Z; Qiu M; Yang Z. 2014. Sp8 plays a supplementary role to Pax6 in establishing the pMN/p3 domain boundary in the spinal cord. Development 141(14):2875-84. PubMed: 24948600MGI: J:213871
- Lin C; Fisher AV; Yin Y; Maruyama T; Veith GM; Dhandha M; Huang GJ; Hsu W; Ma L. 2011. The inductive role of Wnt-beta-Catenin signaling in the formation of oral apparatus. Dev Biol 356(1):40-50. PubMed: 21600200MGI: J:175262
- Lin C; Yao E; Chuang PT. 2015. A conserved MST1/2-YAP axis mediates Hippo signaling during lung growth. Dev Biol 403(1):101-13. PubMed: 25912685MGI: J:224948
- Lin C; Yin Y; Bell SM; Veith GM; Chen H; Huh SH; Ornitz DM; Ma L. 2013. Delineating a conserved genetic cassette promoting outgrowth of body appendages. PLoS Genet 9(1):e1003231. PubMed: 23358455MGI: J:223057
- Lin C; Yin Y; Long F; Ma L. 2008. Tissue-specific requirements of beta-catenin in external genitalia development. Development 135(16):2815-25. PubMed: 18635608MGI: J:139251
- Lin C; Yin Y; Veith GM; Fisher AV; Long F; Ma L. 2009. Temporal and spatial dissection of Shh signaling in genital tubercle development. Development 136(23):3959-67. PubMed: 19906863MGI: J:158288
- Lin HP; Oksuz I; Hurley E; Wrabetz L; Awatramani R. 2015. Microprocessor Complex Subunit DiGeorge Syndrome Critical Region Gene 8 (Dgcr8) Is Required for Schwann Cell Myelination and Myelin Maintenance. J Biol Chem 290(40):24294-307. PubMed: 26272614MGI: J:225866
- Liu Z; Owen T; Zhang L; Zuo J. 2010. Dynamic expression pattern of Sonic hedgehog in developing cochlear spiral ganglion neurons. Dev Dyn 239(6):1674-83. PubMed: 20503364MGI: J:160591
- Lochovska K; Peterkova R; Pavlikova Z; Hovorakova M. 2015. Sprouty gene dosage influences temporal-spatial dynamics of primary enamel knot formation. BMC Dev Biol 15:21. PubMed: 25897685MGI: J:221422
- Lopez-Rios J; Speziale D; Robay D; Scotti M; Osterwalder M; Nusspaumer G; Galli A; Hollander GA; Kmita M; Zeller R. 2012. GLI3 constrains digit number by controlling both progenitor proliferation and BMP-dependent exit to chondrogenesis. Dev Cell 22(4):837-48. PubMed: 22465667MGI: J:184012
- Lu F; Kar D; Gruenig N; Zhang ZW; Cousins N; Rodgers HM; Swindell EC; Jamrich M; Schuurmans C; Mathers PH; Kurrasch DM. 2013. Rax is a selector gene for mediobasal hypothalamic cell types. J Neurosci 33(1):259-72. PubMed: 23283339MGI: J:193919
- Lungova V; Verheyden JM; Herriges J; Sun X; Thibeault SL. 2015. Ontogeny of the mouse vocal fold epithelium. Dev Biol 399(2):263-82. PubMed: 25601450MGI: J:219800
- Mahoney JE; Mori M; Szymaniak AD; Varelas X; Cardoso WV. 2014. The hippo pathway effector Yap controls patterning and differentiation of airway epithelial progenitors. Dev Cell 30(2):137-50. PubMed: 25043473MGI: J:213950
- Maier JA; Harfe BD. 2011. Nuclei Pulposi Formation From the Embryonic Notochord Occurs Normally in GDF-5-Deficient Mice. Spine (Phila Pa 1976) 36(24):E1555-61. PubMed: 21278629MGI: J:178272
- Maier JA; Lo Y; Harfe BD. 2013. Foxa1 and Foxa2 are required for formation of the intervertebral discs. PLoS One 8(1):e55528. PubMed: 23383217MGI: J:195910
- Mao J; Kim BM; Rajurkar M; Shivdasani RA; McMahon AP. 2010. Hedgehog signaling controls mesenchymal growth in the developing mammalian digestive tract. Development 137(10):1721-9. PubMed: 20430747MGI: J:160363
- Mori M; Mahoney JE; Stupnikov MR; Paez-Cortez JR; Szymaniak AD; Varelas X; Herrick DB; Schwob J; Zhang H; Cardoso WV. 2015. Notch3-Jagged signaling controls the pool of undifferentiated airway progenitors. Development 142(2):258-67. PubMed: 25564622MGI: J:238903
- Morimoto M; Nishinakamura R; Saga Y; Kopan R. 2012. Different assemblies of Notch receptors coordinate the distribution of the major bronchial Clara, ciliated and neuroendocrine cells. Development 139(23):4365-73. PubMed: 23132245MGI: J:190886
- Munne PM; Tummers M; Jarvinen E; Thesleff I; Jernvall J. 2009. Tinkering with the inductive mesenchyme: Sostdc1 uncovers the role of dental mesenchyme in limiting tooth induction. Development 136(3):393-402. PubMed: 19141669MGI: J:144193
- Norrie JL; Lewandowski JP; Bouldin CM; Amarnath S; Li Q; Vokes MS; Ehrlich LI; Harfe BD; Vokes SA. 2014. Dynamics of BMP signaling in limb bud mesenchyme and polydactyly. Dev Biol 393(2):270-81. PubMed: 25034710MGI: J:214075
- Nouri N; Awatramani R. 2017. A novel floor plate boundary defined by adjacent En1 and Dbx1 microdomains distinguishes midbrain dopamine and hypothalamic neurons. Development 144(5):916-927. PubMed: 28174244MGI: J:240032
- Nouri N; Patel MJ; Joksimovic M; Poulin JF; Anderegg A; Taketo MM; Ma YC; Awatramani R. 2015. Excessive Wnt/beta-catenin signaling promotes midbrain floor plate neurogenesis, but results in vacillating dopamine progenitors. Mol Cell Neurosci 68:131-42. PubMed: 26164566MGI: J:231849
- Omori A; Harada M; Ohta S; Villacorte M; Sugimura Y; Shiraishi T; Suzuki K; Nakagata N; Ito T; Yamada G. 2011. Epithelial Bmp (Bone morphogenetic protein) signaling for bulbourethral gland development: a mouse model for congenital cystic dilation. Congenit Anom (Kyoto) 51(3):102-9. PubMed: 21848994MGI: J:176595
- Oommen S; Otsuka-Tanaka Y; Imam N; Kawasaki M; Kawasaki K; Jalani-Ghazani F; Anderegg A; Awatramani R; Hindges R; Sharpe PT; Ohazama A. 2012. Distinct roles of MicroRNAs in epithelium and mesenchyme during tooth development. Dev Dyn 241(9):1465-72. PubMed: 22753148MGI: J:186496
- Pan JH; Adair-Kirk TL; Patel AC; Huang T; Yozamp NS; Xu J; Reddy EP; Byers DE; Pierce RA; Holtzman MJ; Brody SL. 2014. Myb permits multilineage airway epithelial cell differentiation. Stem Cells 32(12):3245-56. PubMed: 25103188MGI: J:218553
- Peng T; Frank DB; Kadzik RS; Morley MP; Rathi KS; Wang T; Zhou S; Cheng L; Lu MM; Morrisey EE. 2015. Hedgehog actively maintains adult lung quiescence and regulates repair and regeneration. Nature 526(7574):578-82. PubMed: 26436454MGI: J:226770
- Peng T; Tian Y; Boogerd CJ; Lu MM; Kadzik RS; Stewart KM; Evans SM; Morrisey EE. 2013. Coordination of heart and lung co-development by a multipotent cardiopulmonary progenitor. Nature 500(7464):589-92. PubMed: 23873040MGI: J:204743
- Prochazka J; Pantalacci S; Churava S; Rothova M; Lambert A; Lesot H; Klein O; Peterka M; Laudet V; Peterkova R. 2010. Patterning by heritage in mouse molar row development. Proc Natl Acad Sci U S A 107(35):15497-502. PubMed: 20709958MGI: J:163666
- Prochazka J; Prochazkova M; Du W; Spoutil F; Tureckova J; Hoch R; Shimogori T; Sedlacek R; Rubenstein JL; Wittmann T; Klein OD. 2015. Migration of Founder Epithelial Cells Drives Proper Molar Tooth Positioning and Morphogenesis. Dev Cell 35(6):713-24. PubMed: 26702830MGI: J:239990
- Rock JR; Cecilia Lopez M; Baker HV; Harfe BD. 2007. Identification of genes expressed in the mouse limb using a novel ZPA microarray approach. Gene Expr Patterns 8(1):19-26. PubMed: 17911046MGI: J:127128
- Rockich BE; Hrycaj SM; Shih HP; Nagy MS; Ferguson MA; Kopp JL; Sander M; Wellik DM; Spence JR. 2013. Sox9 plays multiple roles in the lung epithelium during branching morphogenesis. Proc Natl Acad Sci U S A 110(47):E4456-64. PubMed: 24191021MGI: J:202984
- Rodriguez P; Da Silva S; Oxburgh L; Wang F; Hogan BL; Que J. 2010. BMP signaling in the development of the mouse esophagus and forestomach. Development 137(24):4171-6. PubMed: 21068065MGI: J:166768
- Rosello-Diez A; Arques CG; Delgado I; Giovinazzo G; Torres M. 2014. Diffusible signals and epigenetic timing cooperate in late proximo-distal limb patterning. Development 141(7):1534-43. PubMed: 24598165MGI: J:221291
- Runck LA; Method A; Bischoff A; Levitt M; Pena A; Collins MH; Gupta A; Shanmukhappa S; Wells JM; Guasch G. 2014. Defining the molecular pathologies in cloaca malformation: similarities between mouse and human. Dis Model Mech 7(4):483-93. PubMed: 24524909MGI: J:213073
- Sanchez-Camacho C; Bovolenta P. 2008. Autonomous and non-autonomous Shh signalling mediate the in vivo growth and guidance of mouse retinal ganglion cell axons. Development 135(21):3531-41. PubMed: 18832395MGI: J:143442
- Seifert AW; Bouldin CM; Choi KS; Harfe BD; Cohn MJ. 2009. Multiphasic and tissue-specific roles of sonic hedgehog in cloacal septation and external genitalia development. Development 136(23):3949-57. PubMed: 19906862MGI: J:154980
- Seifert AW; Yamaguchi T; Cohn MJ. 2009. Functional and phylogenetic analysis shows that Fgf8 is a marker of genital induction in mammals but is not required for external genital development. Development 136(15):2643-51. PubMed: 19592577MGI: J:158139
- Seifert AW; Zheng Z; Ormerod BK; Cohn MJ. 2010. Sonic hedgehog controls growth of external genitalia by regulating cell cycle kinetics. Nat Commun 1:23. PubMed: 20975695MGI: J:205666
- Snitow ME; Li S; Morley MP; Rathi K; Lu MM; Kadzik RS; Stewart KM; Morrisey EE. 2015. Ezh2 represses the basal cell lineage during lung endoderm development. Development 142(1):108-17. PubMed: 25516972MGI: J:217677
- Song H; Yao E; Lin C; Gacayan R; Chen MH; Chuang PT. 2012. Functional characterization of pulmonary neuroendocrine cells in lung development, injury, and tumorigenesis. Proc Natl Acad Sci U S A 109(43):17531-6. PubMed: 23047698MGI: J:190366
- Tang M; Luo SX; Tang V; Huang EJ. 2013. Temporal and spatial requirements of Smoothened in ventral midbrain neuronal development. Neural Dev 8:8. PubMed: 23618354MGI: J:199133
- Tang M; Miyamoto Y; Huang EJ. 2009. Multiple roles of {beta}-catenin in controlling the neurogenic niche for midbrain dopamine neurons. Development 136(12):2027-38. PubMed: 19439492MGI: J:149534
- Tang M; Villaescusa JC; Luo SX; Guitarte C; Lei S; Miyamoto Y; Taketo MM; Arenas E; Huang EJ. 2010. Interactions of Wnt/beta-catenin signaling and sonic hedgehog regulate the neurogenesis of ventral midbrain dopamine neurons. J Neurosci 30(27):9280-91. PubMed: 20610763MGI: J:161771
- Tang N; Marshall WF; McMahon M; Metzger RJ; Martin GR. 2011. Control of mitotic spindle angle by the RAS-regulated ERK1/2 pathway determines lung tube shape. Science 333(6040):342-5. PubMed: 21764747MGI: J:174194
- Tian H; Jeong J; Harfe BD; Tabin CJ; McMahon AP. 2005. Mouse Disp1 is required in sonic hedgehog-expressing cells for paracrine activity of the cholesterol-modified ligand. Development 132(1):133-42. PubMed: 15576405MGI: J:94270
- Town L; McGlinn E; Fiorenza S; Metzis V; Butterfield NC; Richman JM; Wicking C. 2009. The metalloendopeptidase gene Pitrm1 is regulated by hedgehog signaling in the developing mouse limb and is expressed in muscle progenitors. Dev Dyn 238(12):3175-3184. PubMed: 19877269MGI: J:154373
- Tsao PN; Wei SC; Wu MF; Huang MT; Lin HY; Lee MC; Lin KM; Wang IJ; Kaartinen V; Yang LT; Cardoso WV. 2011. Notch signaling prevents mucous metaplasia in mouse conducting airways during postnatal development. Development 138(16):3533-43. PubMed: 21791528MGI: J:175774
- Varnat F; Zacchetti G; Ruiz I Altaba A. 2010. Hedgehog pathway activity is required for the lethality and intestinal phenotypes of mice with hyperactive Wnt signaling. Mech Dev 127(1-2):73-81. PubMed: 19861162MGI: J:156736
- Veniaminova NA; Vagnozzi AN; Kopinke D; Do TT; Murtaugh LC; Maillard I; Dlugosz AA; Reiter JF; Wong SY. 2013. Keratin 79 identifies a novel population of migratory epithelial cells that initiates hair canal morphogenesis and regeneration. Development 140(24):4870-80. PubMed: 24198274MGI: J:206321
- Verheyden JM; Lewandoski M; Deng C; Harfe BD; Sun X. 2005. Conditional inactivation of Fgfr1 in mouse defines its role in limb bud establishment, outgrowth and digit patterning. Development 132(19):4235-45. PubMed: 16120640MGI: J:101736
- Volckaert T; Dill E; Campbell A; Tiozzo C; Majka S; Bellusci S; De Langhe SP. 2011. Parabronchial smooth muscle constitutes an airway epithelial stem cell niche in the mouse lung after injury. J Clin Invest 121(11):4409-19. PubMed: 21985786MGI: J:178444
- Walton KD; Whidden M; Kolterud A; Shoffner SK; Czerwinski MJ; Kushwaha J; Parmar N; Chandhrasekhar D; Freddo AM; Schnell S; Gumucio DL. 2016. Villification in the mouse: Bmp signals control intestinal villus patterning. Development 143(3):427-36. PubMed: 26721501MGI: J:239983
- Wang DH; Tiwari A; Kim ME; Clemons NJ; Regmi NL; Hodges WA; Berman DM; Montgomery EA; Watkins DN; Zhang X; Zhang Q; Jie C; Spechler SJ; Souza RF. 2014. Hedgehog signaling regulates FOXA2 in esophageal embryogenesis and Barrett's metaplasia. J Clin Invest 124(9):3767-80. PubMed: 25083987MGI: J:215769
- Wang Y; Frank DB; Morley MP; Zhou S; Wang X; Lu MM; Lazar MA; Morrisey EE. 2016. HDAC3-Dependent Epigenetic Pathway Controls Lung Alveolar Epithelial Cell Remodeling and Spreading via miR-17-92 and TGF-beta Signaling Regulation. Dev Cell 36(3):303-15. PubMed: 26832331MGI: J:240083
- Wang Y; Tian Y; Morley MP; Lu MM; Demayo FJ; Olson EN; Morrisey EE. 2013. Development and regeneration of Sox2+ endoderm progenitors are regulated by a Hdac1/2-Bmp4/Rb1 regulatory pathway. Dev Cell 24(4):345-58. PubMed: 23449471MGI: J:195044
- Winkler T; Mahoney EJ; Sinner D; Wylie CC; Dahia CL. 2014. Wnt signaling activates Shh signaling in early postnatal intervertebral discs, and re-activates Shh signaling in old discs in the mouse. PLoS One 9(6):e98444. PubMed: 24892825MGI: J:218511
- Xiao Y; Thoresen DT; Miao L; Williams JS; Wang C; Atit RP; Wong SY; Brownell I. 2016. A Cascade of Wnt, Eda, and Shh Signaling Is Essential for Touch Dome Merkel Cell Development. PLoS Genet 12(7):e1006150. PubMed: 27414798MGI: J:234179
- Xiao Y; Thoresen DT; Williams JS; Wang C; Perna J; Petrova R; Brownell I. 2015. Neural Hedgehog signaling maintains stem cell renewal in the sensory touch dome epithelium. Proc Natl Acad Sci U S A 112(23):7195-200. PubMed: 26015562MGI: J:223401
- Xu J; Liu H; Lan Y; Aronow BJ; Kalinichenko VV; Jiang R. 2016. A Shh-Foxf-Fgf18-Shh Molecular Circuit Regulating Palate Development. PLoS Genet 12(1):e1005769. PubMed: 26745863MGI: J:232140
- Yan CH; Levesque M; Claxton S; Johnson RL; Ang SL. 2011. Lmx1a and lmx1b function cooperatively to regulate proliferation, specification, and differentiation of midbrain dopaminergic progenitors. J Neurosci 31(35):12413-25. PubMed: 21880902MGI: J:176226
- Yang J; Brown A; Ellisor D; Paul E; Hagan N; Zervas M. 2013. Dynamic temporal requirement of Wnt1 in midbrain dopamine neuron development. Development 140(6):1342-52. PubMed: 23444360MGI: J:194842
- Yao Y; Minor PJ; Zhao YT; Jeong Y; Pani AM; King AN; Symmons O; Gan L; Cardoso WV; Spitz F; Lowe CJ; Epstein DJ. 2016. Cis-regulatory architecture of a brain signaling center predates the origin of chordates. Nat Genet 48(5):575-80. PubMed: 27064252MGI: J:231964
- Yin Y; Castro AM; Hoekstra M; Yan TJ; Kanakamedala AC; Dehner LP; Hill DA; Ornitz DM. 2015. Fibroblast Growth Factor 9 Regulation by MicroRNAs Controls Lung Development and Links DICER1 Loss to the Pathogenesis of Pleuropulmonary Blastoma. PLoS Genet 11(5):e1005242. PubMed: 25978641MGI: J:224791
- Yin Y; Wang F; Ornitz DM. 2011. Mesothelial- and epithelial-derived FGF9 have distinct functions in the regulation of lung development. Development 138(15):3169-77. PubMed: 21750028MGI: J:175543
- Yun S; Saijoh Y; Hirokawa KE; Kopinke D; Murtaugh LC; Monuki ES; Levine EM. 2009. Lhx2 links the intrinsic and extrinsic factors that control optic cup formation. Development 136(23):3895-906. PubMed: 19906857MGI: J:154982
- Zhu J; Kwan KM; Mackem S. 2016. Putative oncogene Brachyury (T) is essential to specify cell fate but dispensable for notochord progenitor proliferation and EMT. Proc Natl Acad Sci U S A 113(14):3820-5. PubMed: 27006501MGI: J:232130