Overview

Also Known As: Shhgfpcre

Fluorescence in these mice, consistent with the endogenous Shh locus, is detected in the distal posterior region of the limb buds of embryos aged embryonic day 10 to 12. Mice homozygous for the mutation develop a limited limb skeleton and lack digit 2. This mutant mouse strain may be useful in studies of limb patterning and development.

Donating Investigator

Clifford J Tabin, Harvard Medical School

Genetic overview

Genetic Background	Generation
	N8F1 (2019-03-14 00:00:00)

Shh^{tm1(EGFP/cre)Cjt}

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing, Null/Knockout)	Shh	sonic hedgehog

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Research Applications

Research Tools
Developmental Biology Research

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Base Price

Starting at:

$278.00 Domestic price for female 4-week

348.51 Domestic price for breeder pair

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Details

Detailed Description

This strain expresses a fusion product involving Enhanced Green Fluorescent Protein (EGFP) and Cre recombinase from the endogenous Shh locus. EGFP and cre expression are consistent with the endogenous gene. Fluorescence is detected in the distal posterior region of the limb buds of embryos aged embryonic day 10 to 12 and colocalizes with the endogenous gene product (mRNA).
The donating investigator reports that it is not uncommon for a mosaic expression pattern to be exhibited when the allele is inherited through the female germline. It is recommended that this allele be passed through the male germline when conducting experiments involving cre-induced recombination. Mice homozygous for the mutation develop a limited limb skeleton and lack digit 2. Homozygous mice are not viable or fertile. Heterozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

This mutant mouse strain may be useful in studies of limb patterning and development.

Development

A targeting vector containing a fusion product involving Enhanced Green Fluorescent Protein (EGFP) and Cre recombinase was inserted at the ATG of Shh and disrupted the sequence encoding the first 12 amino acids. The construct was electroporated into 129S6/SvEv-derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and the donating investigator reported that they were then backcrossed to the same for at least 5 generations. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664). The mice were then backcrossed to C57BL/6J (Stock No. 000664) for 8 generations (October 2018).

Expression Data

Expressed Gene	GFP, Green Fluorescent Protein,
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Distal posterior region of the limb buds of embryos aged embryonic day 10 to 12; also a Cre strain.

Control Suggestions

Wild-type from the colony
000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Harfe BD; Scherz PJ; Nissim S; Tian H; McMahon AP; Tabin CJ. 2004. Evidence for an expansion-based temporal Shh gradient in specifying vertebrate digit identities. Cell 118(4):517-28PubMed: 15315763 MGI: J:92504

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Genetics

Shh^{tm1(EGFP/cre)Cjt}

Allele Symbol: Shh^{tm1(EGFP/cre)Cjt}

Allele Name	targeted mutation 1, Clifford J Tabin
Allele Type	Targeted (Recombinase-expressing, Null/Knockout)
Allele Synonym(s)	targeted mutation 1, Clifford J Tabin; Shh^{tm1(EGFP/cre)Cjt}
Gene Symbol and Name	Shh, sonic hedgehog
Gene Synonym(s)	Hhg1; short digits; M100081; Hx; Hxl3; HHG1; HPE3; Hx; Hxl3; SMMCI; HLP3; hedgehog gene 1; hemimelic extra toes; hemimelic extratoes like 3; TPT; TPTPS; MCOPCB5; Dsh; Hhg1; ShhNC
Expressed Gene	GFP, Green Fluorescent Protein,
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Distal posterior region of the limb buds of embryos aged embryonic day 10 to 12; also a Cre strain.
Strain of Origin	Not Specified
Chromosome	5
Molecular Note	A cassette containing an in frame fusion between GFP and cre was inserted at the ATG. Upon recombination, the first 35 base pairs after the ATG were deleted. Fluorescence was detected in the distal posterior region of E10-E12 embryos.
Mutations Made By	Clifford Tabin, Harvard Medical School

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Cre-lox System
  - Cre Recombinase Expression
  - Cre Recombinase Expression: Germline/Embryonic Expression
- Developmental Biology Research
  - Cre-lox System
- Genetics Research
  - Mutagenesis and Transgenesis: Cre-lox System
  - Mutagenesis and Transgenesis
- Fluorescent Proteins
Developmental Biology Research
- Embryonic Lethality (Homozygous)
- Skeletal Defects
- Limb Patterning Defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Shh^{tm1(EGFP/cre)Cjt}/Shh^{tm1(EGFP/cre)Cjt}
Not Specified

cardiovascular system phenotype

abnormal atrium myocardium morphology
- mutants show reduced muscular development in dorsal wall of atria

abnormal heart left atrium morphology

abnormal pulmonary artery morphology
- pulmonary arteries connect to aorta instead of the ouflow tract

abnormal vascular smooth muscle morphology
- reduction in vascular and airway smooth muscle derived from Isl1- and Gli1-positive cardiopulmonary mesoderm progenitors is detected

abnormal vasculogenesis
- embryos have a disorganized vascular endothelial plexus along anterior foregut which does not connect to heart inflow and outflow tracts

absent pulmonary vein
- pulmonary veins from left atrium to lung are absent

growth/size/body region phenotype

acephaly
- fetuses are headless

limbs/digits/tail phenotype

abnormal limb bone morphology
- a limited skeletal structure consisting of the humerus, radius and single digit 1 is seen

liver/biliary system phenotype

abnormal cystic duct morphology
- shortened cystic duct at E14.5

abnormal gallbladder morphology
- formation of smooth muscle layers in gallbladders is delayed and defective

abnormal gallbladder size
- Normal - however, the gallbladder epithelium is histologically normal
- shortened gallbladder at E14.5

abnormal liver development
- fetuses exhibit severe growth retardation in the liver without signs of hepatic inflammation

muscle phenotype

abnormal atrium myocardium morphology
- mutants show reduced muscular development in dorsal wall of atria

abnormal vascular smooth muscle morphology
- reduction in vascular and airway smooth muscle derived from Isl1- and Gli1-positive cardiopulmonary mesoderm progenitors is detected

respiratory system phenotype

abnormal respiratory conducting tube morphology
- airway smooth muscle derived from Isl1- and Gli1-positive cardiopulmonary mesoderm progenitors is significantly reduced; airway smooth muscle development is reduced

skeleton phenotype

abnormal limb bone morphology
- a limited skeletal structure consisting of the humerus, radius and single digit 1 is seen

endocrine/exocrine gland phenotype

abnormal cystic duct morphology
- shortened cystic duct at E14.5

abnormal gallbladder morphology
- formation of smooth muscle layers in gallbladders is delayed and defective

abnormal gallbladder size
- Normal - however, the gallbladder epithelium is histologically normal
- shortened gallbladder at E14.5

References

Harfe BD; Scherz PJ; Nissim S; Tian H; McMahon AP; Tabin CJ. 2004. Evidence for an expansion-based temporal Shh gradient in specifying vertebrate digit identities. Cell 118(4):517-28PubMed: 15315763 MGI: J:92504

Additional References

Liu C; Tate T; Batourina E; Truschel ST; Potter S; Adam M; Xiang T; Picard M; Reiley M; Schneider K; Tamargo M; Lu C; Chen X; He J; Kim H; Mendelsohn CL. 2019. Pparg promotes differentiation and regulates mitochondrial gene expression in bladder epithelial cells. Nat Commun 10(1):4589PubMed: 31597917 MGI: J:281366

Additional - Shh^{tm1(EGFP/cre)Cjt} related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR:Shh^{tm1(EGFP/cre)Cjt}
- Separated MCA:Shh^{tm1(EGFP/cre)Cjt}
- Genotyping resources and troubleshooting
Dietary Information
- New Diet as of March 2015: Lab Diet® 5K0Q (6% fat)
Breeding Considerations

When maintaining a live colony, these mice are bred as heterozygotes. Homozygous mice are not viable.
- Additional Breeding and Husbandry Support
Mating System
- Wild-type x Heterozygote
- Heterozygote x Wild-type
Citation
When using the Shhgfpcre mouse strain in a publication, please cite the originating article(s) and include JAX stock #005622 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX10 (Standard)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Breeder Pair

Sex

Genotype

Price

Female
Male

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or Wild-type for Shh<tm1(EGFP/cre)Cjt>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo	B6.Cg-Shh<tm1(EGFP/cre)Cjt>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6.Cg-Shh<tm1(EGFP/cre)Cjt>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6.Cg-Shh<tm1(EGFP/cre)Cjt>/J Frozen Embryo	$3373.50
Frozen Mouse Embryo	B6.Cg-Shh<tm1(EGFP/cre)Cjt>/J Frozen Embryo	$3373.50

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Also Known As: Shhgfpcre

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Shhtm1(EGFP/cre)Cjt

Allele Symbol: Shhtm1(EGFP/cre)Cjt

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Shhtm1(EGFP/cre)Cjt/Shhtm1(EGFP/cre)CjtNot Specified

cardiovascular system phenotype

growth/size/body region phenotype

limbs/digits/tail phenotype

liver/biliary system phenotype

muscle phenotype

respiratory system phenotype

skeleton phenotype

endocrine/exocrine gland phenotype

References

Additional References

Additional - Shhtm1(EGFP/cre)Cjt related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Breeder Pair

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Shh^{tm1(EGFP/cre)Cjt}

Allele Symbol: Shh^{tm1(EGFP/cre)Cjt}

Genotype: Shh^{tm1(EGFP/cre)Cjt}/Shh^{tm1(EGFP/cre)Cjt}
Not Specified

Additional - Shh^{tm1(EGFP/cre)Cjt} related