Fluorescence in these mice, consistent with the endogenous Shh locus, is detected in the distal posterior region of the limb buds of embryos aged embryonic day 10 to 12. Mice homozygous for the mutation develop a limited limb skeleton and lack digit 2. This mutant mouse strain may be useful in studies of limb patterning and development.
Clifford J Tabin, Harvard Medical School
Genetic Background | Generation |
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N8F4
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Recombinase-expressing, Null/Knockout) | Shh | sonic hedgehog |
Starting at:
$278.00 Domestic price for female 4-week |
348.51 Domestic price for breeder pair |
This strain expresses a fusion product involving Enhanced Green Fluorescent Protein (EGFP) and Cre recombinase from the endogenous Shh locus. EGFP and cre expression are consistent with the endogenous gene. Fluorescence is detected in the distal posterior region of the limb buds of embryos aged embryonic day 10 to 12 and colocalizes with the endogenous gene product (mRNA).
The donating investigator reports that it is not uncommon for a mosaic expression pattern to be exhibited when the allele is inherited through the female germline. It is recommended that this allele be passed through the male germline when conducting experiments involving cre-induced recombination. Mice homozygous for the mutation develop a limited limb skeleton and lack digit 2. Homozygous mice are not viable or fertile. Heterozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
This mutant mouse strain may be useful in studies of limb patterning and development.
A targeting vector containing a fusion product involving Enhanced Green Fluorescent Protein (EGFP) and Cre recombinase was inserted at the ATG of Shh and disrupted the sequence encoding the first 12 amino acids. The construct was electroporated into 129S6/SvEv-derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and the donating investigator reported that they were then backcrossed to the same for at least 5 generations. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664). The mice were then backcrossed to C57BL/6J (Stock No. 000664) for 8 generations (October 2018).
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Distal posterior region of the limb buds of embryos aged embryonic day 10 to 12; also a Cre strain. |
Allele Name | targeted mutation 1, Clifford J Tabin |
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Allele Type | Targeted (Recombinase-expressing, Null/Knockout) |
Allele Synonym(s) | Shhcre/Gfp; ShhCreEGFP; Shhgfpcre; ShhGFP-Cre; Shh-cre; Shhgfpcre; Shh-GFP-Cre; Shhgfp-Cre |
Gene Symbol and Name | Shh, sonic hedgehog |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Distal posterior region of the limb buds of embryos aged embryonic day 10 to 12; also a Cre strain. |
Strain of Origin | Not Specified |
Chromosome | 5 |
Molecular Note | A cassette containing an in frame fusion between GFP and cre was inserted at the ATG. Upon recombination, the first 35 base pairs after the ATG were deleted. Fluorescence was detected in the distal posterior region of E10-E12 embryos. |
Mutations Made By | Clifford Tabin, Harvard Medical School |
When maintaining a live colony, these mice are bred as heterozygotes. Homozygous mice are not viable.
When using the Shhgfpcre mouse strain in a publication, please cite the originating article(s) and include JAX stock #005622 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Shh<tm1(EGFP/cre)Cjt> |
Frozen Mouse Embryo | B6.Cg-Shh<tm1(EGFP/cre)Cjt>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Shh<tm1(EGFP/cre)Cjt>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Shh<tm1(EGFP/cre)Cjt>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Shh<tm1(EGFP/cre)Cjt>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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