B6;D2-Tg(S100B-EYFP)1Wjt/J

Stock number: 005620
Product name: YA
Research areas: Neurobiology Research, Research Tools

Description

These YA mice express a transgene containing Enhanced Yellow Fluorescent Protein (EYFP) that is expressed in neuromuscular junctions.

Detailed description

Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Enhanced Yellow Fluorescent Protein (EYFP) under the direction of the human S100 calcium-binding protein, beta (neural) promoter. Fluorescence is detected in Schwann cells, motor axons, microglia, a subset of Bergmann glia and some muscle macrophages. Strong fluorescent signal is detected in the Schwann cells of sternomastoid, extensor digitorum longus, triangularis sterni and diaphragm muscles and in the motor axons of the triangularis sterni. EYFP expression is detected at birth in glial cells and at postnatal day 7 in motor axons. This mutant mouse strain may be useful in studies of vital imaging of neuronal and glial cells.

Development

A transgenic construct containing an Enhanced Yellow Fluorescent Protein gene under the control of the human S100 calcium-binding protein, beta (neural) promoter, was injected into B6D2F2 donor eggs. Founder line YA was subsequently established and bred to B6D2F2 mice.

Allele

Symbol: Tg(S100B-EYFP)1Wjt
Name: transgene insertion 1, Wesley Thompson
MGI accession ID: MGI:3588511
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(S100B-EYFP)1Wjt
Marker name: transgene insertion 1, Wesley Thompson
Chromosome: UN
Strain of origin: (C57BL/6 x DBA)F2
Expressed genes: YFP,Yellow Fluorescent Protein,jellyfish
Site of expression: Schwann cells, motor axons, microglia, a subset of Bergmann glia and some muscle macrophages. Strong fluorescent signal is detected in the Schwann cells of sternomastoid, extensor digitorum longus, triangularis sterni and diaphragm muscles and in the motor axons of the triangularis sterni. EYFP expression is detected at birth in glial cells and at postnatal day 7 in motor axons.
Molecular note: The transgene contains an Enhanced Yellow Fluorescent Protein gene under the control of the human S100B calcium-binding protein, beta (neural) promoter.