These YA mice express a transgene containing Enhanced Yellow Fluorescent Protein (EYFP) that is expressed in neuromuscular junctions.
Wesley Thompson, University of Texas
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Enhanced Yellow Fluorescent Protein (EYFP) under the direction of the human S100 calcium-binding protein, beta (neural) promoter. Fluorescence is detected in Schwann cells, motor axons, microglia, a subset of Bergmann glia and some muscle macrophages. Strong fluorescent signal is detected in the Schwann cells of sternomastoid, extensor digitorum longus, triangularis sterni and diaphragm muscles and in the motor axons of the triangularis sterni. EYFP expression is detected at birth in glial cells and at postnatal day 7 in motor axons. This mutant mouse strain may be useful in studies of vital imaging of neuronal and glial cells.
A transgenic construct containing an Enhanced Yellow Fluorescent Protein gene under the control of the human S100 calcium-binding protein, beta (neural) promoter, was injected into B6D2F2 donor eggs. Founder line YA was subsequently established and bred to B6D2F2 mice.
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
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Site of Expression | Schwann cells, motor axons, microglia, a subset of Bergmann glia and some muscle macrophages. Strong fluorescent signal is detected in the Schwann cells of sternomastoid, extensor digitorum longus, triangularis sterni and diaphragm muscles and in the motor axons of the triangularis sterni. EYFP expression is detected at birth in glial cells and at postnatal day 7 in motor axons. |
Allele Name | transgene insertion 1, Wesley Thompson |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(S100B-EYFP)1Wjt, transgene insertion 1, Wesley Thompson |
Gene Synonym(s) | |
Promoter | S100B, S100 calcium binding protein B, human |
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
Site of Expression | Schwann cells, motor axons, microglia, a subset of Bergmann glia and some muscle macrophages. Strong fluorescent signal is detected in the Schwann cells of sternomastoid, extensor digitorum longus, triangularis sterni and diaphragm muscles and in the motor axons of the triangularis sterni. EYFP expression is detected at birth in glial cells and at postnatal day 7 in motor axons. |
Strain of Origin | (C57BL/6 x DBA)F2 |
Chromosome | UN |
Molecular Note | The transgene contains an Enhanced Yellow Fluorescent Protein gene under the control of the human S100B calcium-binding protein, beta (neural) promoter. |
Mutations Made By | Wesley Thompson, University of Texas |
When maintaining a live colony, these mice are bred as homozygotes.
When using the YA mouse strain in a publication, please cite the originating article(s) and include JAX stock #005620 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(S100B-EYFP)1Wjt |
Frozen Mouse Embryo | B6;D2-Tg(S100B-EYFP)1Wjt/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;D2-Tg(S100B-EYFP)1Wjt/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;D2-Tg(S100B-EYFP)1Wjt/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6;D2-Tg(S100B-EYFP)1Wjt/J Frozen Embryos | $3373.50 |
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