This strain is currently unavailable due to replenishing of cryopreserved stocks.
These Bace2 knock-out mice exhibit a lack of beta-secretase activity and may be suitable for use in applications related to Alzheimer's disease.
Bart de Strooper, KU Leuven and VIB
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Transcripts derived from the targeted locus do not contain exon 6. One of the enzyme's active sites is encoded by exon 6 so consequently, proteins generated from the targeted locus lack beta-secretase activity. This mutant mouse strain may be useful in studies of Alzheimer's disease.
A targeting vector containing a loxP site and hygromycin resistance gene flanked by FRT sites and was introduced into intron 5. A second loxP site was inserted within intron 6. The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to mice expressing Cre recombinase under the control of the phosphoglycerate kinase promoter to delete exon 6. Heterozygotes were bred to generate homozygotes.
|Allele Name||targeted mutation 1, Bart De Strooper|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||targeted mutation 1, Bart De Strooper; Bace2tm1Bdes|
|Gene Symbol and Name||Bace2, beta-site APP-cleaving enzyme 2|
|Gene Synonym(s)||ALP56; ARP1; ASP21; AEPLC; RIKEN cDNA 1110059C24 gene; 1110059C24Rik; CDA13; ASP1; BAE2; AI850424; 1110059C24Rik; expressed sequence AI850424; DRAP; CEAP1|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||Cre mediated recombination was used to remove exon 6 from the locus.|
|Mutations Made By|| |
Bart de Strooper, KU Leuven and VIB
When maintaining a live colony, these mice are bred as homozygotes.
When using the BACE2 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #005618 in your Materials and Methods section.
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