NOD.C-(Ptprc-D1Mit262)/WehiJ

Stock number: 005616
Product name: NOD.CD45.2
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These congenic mice carry a Chromosome 1 genomic segment from BALB/cWehi and exhibit a diabetes onset and incidence similar to NOD/LtJWehi mice.

Detailed description

This NOD/LtJWehi congenic strain is carrying Chr 1alleles derived from BALB/cWehi including Ptprc^b. Diabetes onset and incidence is similar between NOD.C-(Ptprc-D1Mit262)/WehiJ congenic mice (6/8 by 300 days of age) and NOD/LtJWehi (17/24 by 300 days of age). NOD.C-(Ptprc-D1Mit262)/WehiJ bone marrow transplanted into irradiated NOD/LtJWehi recipients yielded incomplete replacement of donor leukocytes at 16 weeks post transfer versus nearly complete replacement of donor leucocytes in C57BL/6Wehi and B6.SJL/Wehi irradiated mice (Steptoe, et al., 2004, Journal of AutoImmunity, Vol 22, p131-138). This strain provides a unique cell surface marker that can be used to track lineage and phenotype of all hematopoietic donor derived or transferred cells making it useful in studies monitoring diabetes progression.

Development

Ptprc^b a Chr 1 genomic segment from BALB/cWehi was transferred to NOD/LtJWehi (Ptprc^a). An (NODxBALB/c)F1 male was backcrossed to NOD/LtJWehi followed by 10 backcross cycles to NOD/LtJWehi with selection for BALB/c markers on Chr. 1 prior to inbreeding. FACS analysis indicates Ptprc (74cM) is BALB/c in origin (Ptprc^b). Microsatellite analysis indicates D1Mit262 (69.9cM) is BALB/c in origin while D1Mit494 (68.9cM) and D1Mit102 ((75.4cM) are of NOD/LtJWehi origin. Additionally, microsatellite analysis confirms all known Idd alleles (1-20) are NOD in origin (Steptoe, et al., 2004, Journal of AutoImmunity, Vol 22, p131-138). In 2005, the Type 1 Diabetes Resource received this homozygous strain at N10F?

Allele

MGI accession ID: MGI:97810
Marker symbol: Ptprc
Marker name: protein tyrosine phosphatase, receptor type, C
Chromosome: 1

Allele

MGI accession ID: MGI:91647
Marker symbol: D1Mit262
Marker name: DNA segment, Chr 1, Massachusetts Institute of Technology 262
Chromosome: 1