NOD.Cg-Prkdc^scid H2-Ab1^tm1Doi/SzJ

Stock number: 005589
Product name: AΒ^o/o
Research areas: Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools, Virology Research

Detailed description

These mice have no MHC class II expression which makes them useful to facilitate the engraftment of human immune cells.

Development

A construct containing a neomycin expression cassette replaced the second exon of H2-Ab1 was transfected into D3 embryonic stem (ES) cells. These ES cells were injected into C57BL/6 blastocysts. To establish a colony with germ line transmission, chimeric mice were backcrossed to C57BL/6 and then made homozygote for H2-Ab1 mutation (Cosgrove et al, 1991) the mutation was then transferred to the NOD.scid background through 10 generations of backcrossing prior to making homozygote (Schultz et al, unpublished). The T1DR received this strain at N10F10.

Allele

Symbol: H2-Ab1^tm1Doi
Name: targeted mutation 1, Christophe Benoist and Diane Mathis
MGI accession ID: MGI:1927483
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: H2-Ab1
Marker name: histocompatibility 2, class II antigen A, beta 1
Chromosome: 17
Strain of origin: 129S2/SvPas
Molecular note: The second exon was disrupted by the insertion of a neomycin resistance gene. In addition, the ES cell line used was derived from the 129S2/SvPas strain, which carries a deletion in the promoter region of H2-Ea. Consequently, these MHC class II molecule-deficient mice lacked cell surface expression of both class II-A and class II-E MHC proteins.

Allele

Symbol: Prkdc^scid
Name: severe combined immunodeficiency
MGI accession ID: MGI:1857113
Generation method: Spontaneous
Marker symbol: Prkdc
Marker name: protein kinase, DNA activated, catalytic polypeptide
Chromosome: 16
Strain of origin: C.BKa-Igh^b/Icr
Site of expression: T and B lymphocytes.
Molecular note: A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*).