B6.Cg-Tg(Prrx1-cre)1Cjt/J

Stock number: 005584
Product name: Prrx1^Cre , Prx1-Cre , Prx1^cre , B6J.Prrx1^Cre , B6J.Prrx1-Cre
Research areas: Developmental Biology Research, Research Tools

Description

Prx1-Cre (Prrx1^Cre) transgenic mice have the Prrx1 promoter/enhancer directing Cre recombinase expression to early limb bud mesenchyme and a subset of craniofacial mesenchyme. This tool strain may be useful for generating tissue specific-targeted mutants for studying limb bud development and patterning.

Detailed description

Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the paired related homeobox 1 promoter. Cre recombinase expression closely patterns endogenous gene expression and is detectable by embryonic day 9.5. Cre recombinase activity is expected in early limb bud mesenchyme and in a subset of craniofacial mesenchyme. Limited Cre expression may also be observed in female germline (oocytes) and male reproductive tissue (Leydig cells). If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry (Tg(Prrx1-cre)1Cjt). The source data may also be examined (view Cre expression characterization). This same information would also be found searching the MGI Recombinase Activity database.

When Prrx1^Cre transgenic mice are crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the floxed sequence in early limb bud mesenchyme. This strain represents an effective tool for generating tissue specific-targeted mutants useful in studies of limb bud development and patterning.

Development

A transgenic construct containing cre coding sequence under the control of the paired related homeobox 1 promoter was microinjected into B6SJLF2 donor oocytes. Founder animals were backcrossed to C57BL/6J for 12 generations.

Allele

Symbol: Tg(Prrx1-cre)1Cjt
Name: transgene insertion 1, Clifford J Tabin
MGI accession ID: MGI:2450929
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Prrx1-cre)1Cjt
Marker name: transgene insertion 1, Clifford J Tabin
Chromosome: UN
Strain of origin: (C57BL/6J x SJL/J)F2
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: early limb bud mesenchyme and in a subset of craniofacial mesenchyme, some female germline expression
Molecular note: This transgene expresses Cre recombinase under the control of a Prrx1 derived enhancer. This transgene is expressed in the early limb bud mesenchyme and in a subset of craniofacial mesenchyme. A germline Cre recombinase activity was observed in female, but not in male, as partially penetrant trait depending on the particular gene flanked by loxP sites.