Prx1-Cre (Prrx1Cre) transgenic mice have the Prrx1 promoter/enhancer directing Cre recombinase expression to early limb bud mesenchyme and a subset of craniofacial mesenchyme. This tool strain may be useful for generating tissue specific-targeted mutants for studying limb bud development and patterning.
Clifford J Tabin, Harvard Medical School
Genetic Background | Generation |
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N12+N3pN2F12
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Allele Type |
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Transgenic (Recombinase-expressing) |
Starting at:
$278.00 Domestic price for female 4-week |
348.51 Domestic price for breeder pair |
Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the paired related homeobox 1 promoter. Cre recombinase expression closely patterns endogenous gene expression and is detectable by embryonic day 9.5. Cre recombinase activity is expected in early limb bud mesenchyme and in a subset of craniofacial mesenchyme. Limited Cre expression may also be observed in female germline (oocytes) and male reproductive tissue (Leydig cells). If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry (Tg(Prrx1-cre)1Cjt). The source data may also be examined (view Cre expression characterization). This same information would also be found searching the MGI Recombinase Activity database.
When Prrx1Cre transgenic mice are crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the floxed sequence in early limb bud mesenchyme. This strain represents an effective tool for generating tissue specific-targeted mutants useful in studies of limb bud development and patterning.
A transgenic construct containing cre coding sequence under the control of the paired related homeobox 1 promoter was microinjected into B6SJLF2 donor oocytes. Founder animals were backcrossed to C57BL/6J for 12 generations.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | early limb bud mesenchyme and in a subset of craniofacial mesenchyme, some female germline expression |
Allele Name | transgene insertion 1, Clifford J Tabin |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | Prx-1 Cre; Prx1cre; Prx1Cre; Prx1-cre; Prx-cre; Tg[Prx1-cre]1Cjt |
Gene Symbol and Name | Tg(Prrx1-cre)1Cjt, transgene insertion 1, Clifford J Tabin |
Gene Synonym(s) | |
Promoter | Prrx1, paired related homeobox 1, rat |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | early limb bud mesenchyme and in a subset of craniofacial mesenchyme, some female germline expression |
Strain of Origin | (C57BL/6J x SJL/J)F2 |
Chromosome | UN |
Molecular Note | This transgene expresses Cre recombinase under the control of a Prrx1 derived enhancer. This transgene is expressed in the early limb bud mesenchyme and in a subset of craniofacial mesenchyme. A germline Cre recombinase activity was observed in female, but not in male, as partially penetrant trait depending on the particular gene flanked by loxP sites. |
Mutations Made By | Malcolm Logan, National Institute for Medical Research |
When maintaining a live colony, hemizygous mice are bred to wildtype (noncarrier) mice from the colony or with C57BL/6J inbred mice (Stock No. 000664). Homozygous mice, reportedly, are viable and fertile.
Of note, the Prrx1Cre transgenic mice may exhibit limited Cre expression in female germline (oocytes) and male reproductive tissue (Leydig cells). See strain description for more details. If breeding to mouse lines with a floxed allele, to avoid germline deletion of the floxed allele, researchers may consider breeding females with the floxed allele to Prrx1Cre transgenic males.
When using the Prrx1Cre , Prx1-Cre , Prx1cre , B6J.Prrx1Cre , B6J.Prrx1-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #005584 in your Materials and Methods section.
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Prrx1-cre)1Cjt/ |
Frozen Mouse Embryo | B6.Cg-Tg(Prrx1-cre)1Cjt/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Prrx1-cre)1Cjt/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Prrx1-cre)1Cjt/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Prrx1-cre)1Cjt/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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