These floxed mutant mice possess loxP sites flanking exon 1 of the Rac1 gene. This strain may be useful for generating conditional mutations to elucidate the role of Rac1 in various tissues.
David J. Kwiatkowski, Brigham and Women's Hospital
These mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.
When bred to a strain expressing Cre recombinase in the myeloid cell lineage (see Stock No. 004781 for example), this mutant mouse strain may be useful in studies of neutrophil function.
When bred to a strain expressing a tamoxifen inducible Cre recombinase specific to keratinocytes(see Stock No. 005107 for example), this mutant mouse strain may be useful in studies of stem cell renewal in the epidermis.
A loxP site flanked targeting vector containing a neomycin resistance gene was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 1 of the targeted gene, and another loxP site was inserted upstream of exon 1. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells which were transiently transfected with a vector expressing Cre to remove the selection cassette. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting mice were then crossed to a mixed background (129S4/SvJae, BALB/c, C57BL/6).
|Allele Name||targeted mutation 1, David J Kwiatkowski|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Rac1c; Rac1flox; Rac1tm1Glog|
|Gene Symbol and Name||Rac1, Rac family small GTPase 1|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A loxP site was inserted upstream of exon 1 and a loxP site flanked neomycin resistance gene cassette into intron 1. The neo cassette was removed through subsequent cre-mediated recombination, leaving exon 1 floxed.|
|Mutations Made By|| |
David Kwiatkowski, Brigham and Women's Hospital
When maintaining a live colony, these mice are bred as homozygotes.
When using the Rac1flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #005550 in your Materials and Methods section.