B6.129P-Ly75^tm1Mnz/J

Stock number: 005528
Product name: DEC-205 KO
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These Ly75 knock-out mice exhibit an absence of a cytotoxic lymphocyte response to immunization with foreign splenocytes.

Detailed description

Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Flow cytometry analysis of activated T cells does not detect a functional gene product (protein). Splenic CD8a+ dendritic cells occur at slightly higher levels while CD8- dendritic cells occur at slightly lower levels in homozygotes. Cytotoxic lymphocyte response to immunization with foreign splenocytes is absent. This mutant mouse strain may be useful in studies of dendritic cells and innate immunity.

Development

A targeting vector was used to disrupt the promoter and exon 1. The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to the same for 12 generations.

Allele

Symbol: Ly75^tm1Mnz
Name: targeted mutation 1, Michel C Nussenzweig
MGI accession ID: MGI:3575119
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: DEC205 ko; DEC-205^-; DEC-205^null
Marker symbol: Ly75
Marker name: lymphocyte antigen 75
Marker synonyms: CD205; CLEC13B; DEC-205; gp200-MR6; LY-75; RGD1565962
Chromosome: 2
Strain of origin: 129P2/OlaHsd
Molecular note: A targeting vector was used to disrupt the promoter and exon 1. Flow cytometry analysis of activated T cells does not detect a functional gene product (protein).