These Ly75 knock-out mice exhibit an absence of a cytotoxic lymphocyte response to immunization with foreign splenocytes.
Michel C Nussenzweig, The Rockefeller University
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Flow cytometry analysis of activated T cells does not detect a functional gene product (protein). Splenic CD8a+ dendritic cells occur at slightly higher levels while CD8- dendritic cells occur at slightly lower levels in homozygotes. Cytotoxic lymphocyte response to immunization with foreign splenocytes is absent. This mutant mouse strain may be useful in studies of dendritic cells and innate immunity.
A targeting vector was used to disrupt the promoter and exon 1. The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to the same for 12 generations.
|Allele Name||targeted mutation 1, Michel C Nussenzweig|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||DEC205 ko; DEC-205-; DEC-205null|
|Gene Symbol and Name||Ly75, lymphocyte antigen 75|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A targeting vector was used to disrupt the promoter and exon 1. Flow cytometry analysis of activated T cells does not detect a functional gene product (protein).|
|Mutations Made By|| |
Kang Liu, The Rockefeller University
When maintaining a live colony, these mice are bred as homozygotes.
When using the DEC-205 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #005528 in your Materials and Methods section.