Transgenic mice reportedly express the mutant Ins2*Y16A protein in the pancreatic islets and thymus. The donating investigator reports approximately 75% of female transgenic mice (founder line B) become diabetic in the presence of native insulin genes by 35 weeks of age. In contrast, NOD female transgenic mice lacking both Ins1 and Ins2 fail to produce insulin autoantibodies, and neither diabetes nor insulitis develops by 26 weeks of age. Sialitis does occur, however. Line B transgenics have lower expression levels than line F (see Stock No. <a href="https://www.jax.org/strain/005525">005525</a>), and 50% of the line B male transgenics lacking both Ins1 and Ins2 develop metabolic diabetes, with little to no insulitis, before 10 weeks of age.
This strain is useful to study insulin-reactive autoimmunity.

These double knock-out Line B mice carry two knock-out insulin genes, Ins1 and Ins2 along with a transgene expressing a mutated insulin. Female transgenic mice fail to produce insulin autoantibodies, and show no diabetes or insulitis. Sialitis is expressed at a lower level than Line F (Stock No. <a href="https://www.jax.org/strain/005525"">005525</a>).

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