Diabetes onset and incidence among these congenic mice is significantly later and reduced compared to NOD/Lt mice.
Dr. David Serreze, The Jackson Laboratory
|Allele Type||Gene Symbol||Gene Name|
|QTL||Idd9||insulin dependent diabetes susceptibility 9|
|Marker Symbol||Marker Name|
|D4Mit31||DNA segment, Chr 4, Massachusetts Institute of Technology 31|
|D4Mit310||DNA Segment, Chr 4, Massachusetts Institute of Technology 310|
This NOD/Lt congenic strain is carrying chr 4 alleles derived from strain NOR/Lt at the insulin dependent diabetes susceptibility 9 and 11 loci. Diabetes onset and incidence among NOD.NOR-D4Mit31-D4Mit310 congenic mice is significantly later and reduced. Similar to the Irvakine et al, 2005 publication it has been reported that this NOD.NOR- Idd9/11 congenic stock is resistant to Cyclophosphamide induced insulitis in both males and females.
This strain is useful for dissecting the role of non-MHC susceptibility genes in autoimmune diabetes.
Genomic segments of Chr 4 extending from D4Mit31 (51.3cM) through D4Mit310 (21.9) found in earlier linkage studies to include diabetogenic loci Idd9 (42.6cM) and Idd11 (64.6cM) transferred from NOR/Lt to NOD/Lt. This NOR/Lt derived chromosomal segment is of C57BL/6J and DBA origin. Siblings carrying the D4Mit31- D4Mit310 from NOR/Lt were intercrossed to make homozygote. Additional microsatellite analysis confirm all other known Idd regions are NOD/Lt in origin. In 2005, The T1DR received this stock at generation N5F11.
|Gene Symbol and Name||Idd9, insulin dependent diabetes susceptibility 9|
|Strain of Origin||NOR/LtJ|
|Molecular Note||This allele confers resistance to cyclophosphamide-induced insulitis compared to NOD/Jsd.|
A footpad injection of Complete Freund s Adjuvant (CFA) administered once at weaning will delay diabetes onset, thus extending the lifespan of breeders. Use of Complete Freund s Adjuvant in NOD mice can be found in Current Protocols in Immunology page 15.9.19, Reproduction.
When using the NOD.Idd9/11NOR CH4NOR mouse strain in a publication, please cite the originating article(s) and include JAX stock #005510 in your Materials and Methods section.