Mice that are homozygous for the targeted allele and hemizygous for the transgene express all six isoforms (including both 3R and 4R forms) of human microtubule-associated protein tau (MAPT). These mutant mice may be useful in studies examining the relationship between human MAPT and Alzheimer's disease pathogenesis
Karen Duff, Nathan Kline Institute
Peter Davies, Albert Einstein College of Medicine
Genetic Background | Generation |
---|---|
N9+N2F15
|
Allele Type |
---|
Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Reporter, Null/Knockout) | Mapt | microtubule-associated protein tau |
Starting at:
$255.00 Domestic price for female 5-week |
510.00 Domestic price for breeder pair |
Mice that are homozygous for the targeted allele and hemizygous for the transgene are viable and fertile. Although no endogenous mouse MAPT is detected, all six isoforms (including both 3R and 4R forms) of human MAPT are expressed. Hyperphosphorylated MAPT is detected in cell bodies and dendrites by three months of age. Paired helical filaments of aggregated insoluble MAPT can be isolated from brain tissue as early as two months of age. These mutant mice may be useful in studies examining the relationship between human MAPT and Alzheimer's disease pathogenesis. The Donating Investigator reports that mice homozygous for the transgenic insert have never been observed and that such mice are presumed to be embryonic lethal.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Double mutant mice were generated by crossing transgenic mice (mouse line 8C) with Mapt targeted mutant mice. The targeted mutant allele was created by inserting EGFP coding sequence into the first Mapt exon, disrupting expression of the Mapt gene and producing a cytoplasmic EGFP protein fused to the first 31 MAPT amino acids (see Stock Number 004779). The transgenic allele consists of a PAC insert of 200-250 kb that includes the coding sequence, intronic regions and regulatory elements of the human MAPT gene. The targeted allele was created in 129S4/SvJae-derived J1 embryonic stem cells which were subsequently injected into C57BL/6 blastocysts. The transgenic allele was generated in embryos derived from a cross between Swiss Webster females and B6D2F1 males. Mice containing both alleles were crossed to C57BL/6 mice for nine generations by the donating investigator. Upon arrival at The Jackson Laboratory, the mice were crossed once more to C57BL/6 (N10). Offspring were mated to generate mice that are homozygous at the targeted mutant allele.
Expressed Gene | MAPT, microtubule associated protein tau, human |
---|---|
Site of Expression | Neurons: EGFP signal observed at 9.0 dpc (days post-coitum) in the trilgeminal ganglion. At 10.75 dpc. EGFP was seen throughout the developing nervous system. |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | GFP signal is detected beginning a embryonic day 9 in the trigeminal ganglion and throughout the developing central nervous system by embryonic day 10.75. GFP expression persists in adults and closely resembles the expression of neuron specific beta-tubulin III. |
Allele Name | transgene insertion 8c, Peter Davies |
---|---|
Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | Tg(MAPT)8cPdav; transgene insertion 8c, Peter Davies |
Gene Symbol and Name | Tg(MAPT)8cPdav, transgene insertion 8c, Peter Davies |
Gene Synonym(s) | 8c |
Promoter | MAPT, microtubule associated protein tau, human |
Expressed Gene | MAPT, microtubule associated protein tau, human |
Site of Expression | Neurons: EGFP signal observed at 9.0 dpc (days post-coitum) in the trilgeminal ganglion. At 10.75 dpc. EGFP was seen throughout the developing nervous system. |
Strain of Origin | Swiss Webster x (C57BL/6 x DBA)F1 |
Chromosome | UN |
General Note | Mice that are homozygous for the Mapttm1(GFP)Klt allele and hemizygous for the transgene are viable and fertile. Although no endogenous mouse MAPT is detected, all six isoforms (including both 3R and 4R forms) of human MAPT are expressed. Hyperphosphorylated MAPT is detected in cell bodies and dendrites by 3 months of age. Paired helical filaments of aggregated insoluble MAPT can be isolated from brain tissue as early as 2 months of age. |
Molecular Note | The transgene contains the coding sequence, intronic regions, and regulatory elements of the human microtubule-associated protein tau (MAPT) gene. |
Mutations Made By | Peter Davies, Albert Einstein College of Medicine |
Allele Name | targeted mutation 1, Kerry Lee Tucker |
---|---|
Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | targeted mutation 1, Kerry Lee Tucker; Mapttm1(EGFP)Klt |
Gene Symbol and Name | Mapt, microtubule-associated protein tau |
Gene Synonym(s) | RNPTAU; MSTD; FTDP-17; Mtapt; pTau; Mtapt; MTBT1; AI413597; PPND; PPP1R103; DDPAC; Tau; expressed sequence AI413597; MTBT2; expressed sequence AW045860; MAPTL; TAU; AW045860; Tau |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | GFP signal is detected beginning a embryonic day 9 in the trigeminal ganglion and throughout the developing central nervous system by embryonic day 10.75. GFP expression persists in adults and closely resembles the expression of neuron specific beta-tubulin III. |
Strain of Origin | 129S4/SvJae |
Chromosome | 11 |
Molecular Note | Exon 1 was disrupted by the insertion of a cassette encoding the EGFP fluorescent marker gene and a neomycin resistance gene. Fusion protein including EGFP was detected by Western blot analysis of homozygous mutant brain lysates. Fluorescent illumination indicated that expression of the reporter gene was similar to that of the endogenous gene. |
Mutations Made By | Dr. Kerry Tucker, Ruprecht Karls University of Heidelberg |
When maintaining a live colony, mice that are homozygous for the targeted mutation and hemizygous for the transgene are bred with mice homozygous for the targeted mutation and wildtype for the transgene. The Donating Investigator reports that mice homozygous for the transgenic insert have never been observed and that such mice are presumed to be embryonic lethal.
or
Homozygous for Mapttm1(EGFP)Klt, Noncarrier x Homozygous for Mapttm1(EGFP)Klt, Hemizygous for Tg(MAPT)8cPda
When using the htau mouse strain in a publication, please cite the originating article(s) and include JAX stock #005491 in your Materials and Methods section.
Service | Genotype | Price |
---|---|---|
Heterozygous for Mapt<tm1(EGFP)Klt>, Hemizygous or Non carrier for Tg(MAPT)8cPdav |
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
Frozen Mouse Embryo | B6.Cg-Mapt<tm1(EGFP)Klt> Tg(MAPT)8cPdav/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Mapt<tm1(EGFP)Klt> Tg(MAPT)8cPdav/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Mapt<tm1(EGFP)Klt> Tg(MAPT)8cPdav/J | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Mapt<tm1(EGFP)Klt> Tg(MAPT)8cPdav/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.
MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.
Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.