Fluorescence from yellow fluorescent protein is observed in virtually all cell types in all tissues in these transgenic mice.
Andras Nagy, Lunenfeld-Tanenbaum Research Institute (LTRI), Mount Sinai Hospital
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
Mice that are homozygous for the transgenic insert (founder line 7AC5/EYFP) are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. All tissues from hemizygous animals display fluorescence in all cell types under appropriate lighting conditions. Homozygotes fluoresce with twice the intensity of the hemizygotes. Notable exceptions to this phenotype are erythrocytes and adipocytes in which fluorescence is negligible or absent.
Please note, the original publication describing the creation and phenotype of mice harboring the pCX::EFYP transgene (Hadjantonakis 2002 BMC Biotechnol 2002 2:11) describes mice from founder line "YC5/EYFP" and indicates they are available through The Jackson Laboratory as Stock No. 003772. While the 003772 strain is no longer available, the donating investigators report that this Stock No. 005483 strain (from founder line "7AC5/EYFP") was generated from embryonic stem cell clones from the same experiment using the identical pCX::EYFP vector. Although the transgene integrated independently in the two founder lines, the donating investigators report that mice derived from lines YC5/EYFP and 7AC5/EYFP are "phenotypically indistinguishable."
A transgenic construct (pCX::EYFP) containing an Enhanced Yellow Fluorescent Protein gene (ClonTech) under the control of a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer, was introduced into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Many different ES cell clones were identified containing the construct (including line YC5/EYFP and 7AC5/EYFP). For these mice, 7AC5/EYFP clones were aggregated with ICR morulae and the resulting chimeric males were bred with ICR females to test for germline transmission. Chimeric mice found to be capable of germline transmission were themselves bred to 129S1 females. The transgenic offspring were intercrossed to generate homozygotes. These mice, derived from founder line 7AC5/EYFP ("phenotypically indistinguishable" from the originally published YC5/EYFP founder line), were sent by the Dr. Andras Nagy research group at Mount Sinai Hospital to The Jackson Laboratory in 2005.
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
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Site of Expression | Widespread expression with the noted exception of adipocytes and erythrocytes. |
Allele Name | transgene insertion 7AC5, Andras Nagy |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | 7AC5/EYFP; Tg(ACTB-EYFP)7AC5Nagy; Tg(CAG-EYFP)7AC5Nagy |
Gene Symbol and Name | Tg(CAG-EYFP)7AC5Nagy, transgene insertion 7AC5, Andras Nagy |
Gene Synonym(s) | |
Promoter | ACTB, actin, beta, chicken |
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
Site of Expression | Widespread expression with the noted exception of adipocytes and erythrocytes. |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | UN |
Molecular Note | The transgene construct is composed of an enhanced yellow fluorescent protein (EYFP) under control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate-early enhancer. |
Mutations Made By | Andras Nagy, Lunenfeld-Tanenbaum Research Institute (LTRI), Mount Sinai Hospital |
When using the 7AC5/EYFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #005483 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(CAG-EYFP)7AC5Nagy |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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