129-Tg(CAG-EYFP)7AC5Nagy/J

Stock number: 005483
Product name: 7AC5/EYFP
Research areas: Research Tools

Description

Fluorescence from yellow fluorescent protein is observed in virtually all cell types in all tissues in these transgenic mice.

Detailed description

Mice that are homozygous for the transgenic insert (founder line 7AC5/EYFP) are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. All tissues from hemizygous animals display fluorescence in all cell types under appropriate lighting conditions. Homozygotes fluoresce with twice the intensity of the hemizygotes. Notable exceptions to this phenotype are erythrocytes and adipocytes in which fluorescence is negligible or absent.

Please note, the original publication describing the creation and phenotype of mice harboring the pCX::EFYP transgene (Hadjantonakis 2002 BMC Biotechnol 2002 2:11) describes mice from founder line "YC5/EYFP" and indicates they are available through The Jackson Laboratory as Stock No. 003772. While the 003772 strain is no longer available, the donating investigators report that this Stock No. 005483 strain (from founder line "7AC5/EYFP") was generated from embryonic stem cell clones from the same experiment using the identical pCX::EYFP vector. Although the transgene integrated independently in the two founder lines, the donating investigators report that mice derived from lines YC5/EYFP and 7AC5/EYFP are "phenotypically indistinguishable."

Development

A transgenic construct (pCX::EYFP) containing an Enhanced Yellow Fluorescent Protein gene (ClonTech) under the control of a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer, was introduced into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Many different ES cell clones were identified containing the construct (including line YC5/EYFP and 7AC5/EYFP). For these mice, 7AC5/EYFP clones were aggregated with ICR morulae and the resulting chimeric males were bred with ICR females to test for germline transmission. Chimeric mice found to be capable of germline transmission were themselves bred to 129S1 females. The transgenic offspring were intercrossed to generate homozygotes. These mice, derived from founder line 7AC5/EYFP ("phenotypically indistinguishable" from the originally published YC5/EYFP founder line), were sent by the Dr. Andras Nagy research group at Mount Sinai Hospital to The Jackson Laboratory in 2005.

Allele

Symbol: Tg(CAG-EYFP)7AC5Nagy
Name: transgene insertion 7AC5, Andras Nagy
MGI accession ID: MGI:3687017
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(CAG-EYFP)7AC5Nagy
Marker name: transgene insertion 7AC5, Andras Nagy
Chromosome: UN
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Expressed genes: YFP,Yellow Fluorescent Protein,jellyfish
Site of expression: Widespread expression with the noted exception of adipocytes and erythrocytes.
Molecular note: The transgene construct is composed of an enhanced yellow fluorescent protein (EYFP) under control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate-early enhancer.