These mice carry an ENU-induced mutation characterized by body tremors and muscle spasms.
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The mutants show body tremor, with intermittent muscle spasms visible across the area of the lower back and hind limbs, and slight trunk swaying; hind limbs are lifted high during walking. This phenotype becomes visible at approximately 4 weeks of age (average 3.7+/-1.3 weeks of age; n=55). A complementation test with Grid2ho-15J (JR#005344; homozygous x heterozygous mating) resulted in 7 affected mice in a total of 16 progeny and therefore demonstrates that nmf334 represents an allele of Grid2. Standard pathology work-up on two mutants (24 days of age) revealed no abnormalities. Mutants breed and a colony can be maintained through normal breeding.
This phenotypic deviant was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males (Stock No. 000664), in the Neuroscience Mutagenesis facility at The Jackson Laboratory. Mutagenized males were crossed to C57BL/6J females; G3 descendants of the mutagenized males were selected for neurological impairment.
|Allele Name||hotfoot 16 Jackson|
|Allele Type||Chemically induced (ENU)|
|Allele Synonym(s)||Grid2nmf334; neuroscience mutagenesis facility, 334; NMF334|
|Gene Symbol and Name||Grid2, glutamate receptor, ionotropic, delta 2|
|Strain of Origin||C57BL/6J|
|Molecular Note||This phenotypic mutation, identified in an ENU mutagenesis screen, was shown to be an allele of Grid2 by a complementation test.|