These mice carry an ENU-induced mutation that causes vestibular defects due to a lack of otoconia, and deficient neutrophil responses.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Not Applicable | Tyr | tyrosinase |
Allele Type | Gene Symbol | Gene Name |
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Chemically induced (ENU) | Cyba | cytochrome b-245, alpha polypeptide |
These mutants were detected through routine inspections for overt abnormal behavior; head- and body tilt can be observed at approximately 4 weeks of age (average onset 4.6 +/- 0.6 weeks; n=15). Standard pathology work-up and a whole-mount ear exam on one mutant (56 days of age) revealed no abnormalities. Male or female mutants have been produced and the colony is maintained through regular breeding.
This phenotypic deviant was generated by ethylnitrosourea (ENU) mutagenesis in A.B6-Tyr+/J males (Stock No. 002565), in the Neuroscience Mutagenesis facility at The Jackson Laboratory.
Allele Name | neuroscience mutagenesis facility 333 |
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Allele Type | Chemically induced (ENU) |
Allele Synonym(s) | p22(phox)-; p22phox- |
Gene Symbol and Name | Cyba, cytochrome b-245, alpha polypeptide |
Gene Synonym(s) | |
Strain of Origin | A.B6-Tyr+/J |
Chromosome | 8 |
Molecular Note | This mutation was identified in an ENU mutagenesis screen. Sequence analysis of the Cyba gene of nmf333 mice revealed a T to C point mutation in exon 5, resulting in a tyrosine to histidine substitution at amino acid position 121 of the encoded protein. The mutated tyrosine is located within a predicted transmembrane domain of the protein and is likely to have profound structural consequences. The identified point mutation did not diminish mRNA expression; RT-PCR analysis revealed that the mutant transcript was present at normal levels in the spleens of mutant mice. However, Western blot experiments demonstrated that mature protein was absent from the neutrophil granulocytes of homozygous nmf333 mice while expression was readily detected in theneutrophils of control littermates. |
When using the A.B6 Tyr+-Cybanmf333/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #005445 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Homozygous for Cyba<nmf333>, 1 pair minimum |
Frozen Mouse Embryo | A.B6 Tyr<+>-Cyba<nmf333>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | A.B6 Tyr<+>-Cyba<nmf333>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | A.B6 Tyr<+>-Cyba<nmf333>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | A.B6 Tyr<+>-Cyba<nmf333>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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