Mecp2308 mice have a loxP-flanked neomycin resistance and herpes simplex virus thymidine kinase genes that disrupt exon 4, with a stop codon inserted after Mecp2 codon 308. By 6 weeks of age, male mutant mice of this strain begin to exhibit tremors, progressive motor dysfunction, oily disheveled fur, hypoactivity, myoclonic seizures, and kyphosis. This mutant mouse strain may be useful in studies of Rett Syndrome.
Huda Zoghbi, Baylor College of Medicine
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Not Applicable) | Mecp2 | methyl CpG binding protein 2 |
Mice that are homozygous for the targeted mutation are viable and fertile. A truncated gene product (protein) is detected by immunohistochemical analysis of brain tissue. By 6 weeks of age, male mutant mice begin to exhibit tremors, progressive motor dysfunction, oily disheveled fur, hypoactivity, myoclonic seizures, and kyphosis. Approximately 10% of male mutants die between 10 and 12 months of age. Heterozygous female mice exhibit a milder phenotype. All mutant male mice and 62% of female heterozygotes exhibit a repetitive clasping movement of their forelimbs and exhibit tremors. The Donating Investigator reports that the myoclonic seizures, kyphosis and reduced survival were observed in aged male mutants on a 129/SvEv genetic background. This mutant mouse strain may be useful in studies of Rett Syndrome.
The Howard Hughes Medical Institute and the National Institutes of Health supported the creation of this model. Importation of this model was supported by the Rett Syndrome Research Foundation.
A targeting vector containing loxP-flanked neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 4, with a stop codon inserted after codon 308. The construct was electroporated into 129S7/SvEvBrd-Hprtb-m2derived AB2.2 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice, and then backcrossed to the same for 12 generations.
Allele Name | targeted mutation 1, Huda Zoghbi |
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Allele Type | Targeted (Not Applicable) |
Allele Synonym(s) | Mecp2308 |
Gene Symbol and Name | Mecp2, methyl CpG binding protein 2 |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprtb-m2 |
Chromosome | X |
Molecular Note | A stop codon and a floxed neo cassette were inserted into exon 4. The stop codon was inserted downstream of codon 308, allowing translation of the methyl-CpG binding domain and the transcriptional repression domain. Western blot analysis showed that only truncated protein was present in homozygous mutant mice. Staining of brain tissue with a carboxy terminal antibody confirmed the absence of normal protein. |
Mutations Made By | Huda Zoghbi, Baylor College of Medicine |
Can be maintained female homozygote mated to hemizygote male, this mating scheme tends to have a lower productivy rate. The breeding scheme of heterozygote female mated to hemizygote male increases rate of productivity.
When using the Mecp2308 mouse strain in a publication, please cite the originating article(s) and include JAX stock #005439 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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X linked - Heterozygous Females and Wild-type Males for Mecp2<tm1Hzo> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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