B6.129P2-Ccr5^tm1Kuz/J

Stock number: 005427
Product name: CCR5 KO
Research areas: Cancer Research, Immunology, Inflammation and Autoimmunity Research

Description

Absence of chemokine (C-C motif) receptor 5 in these knock-out mice affects immune and a number of other physiological responses. They are useful for widespread studies of innate immunity.

Detailed description

At birth, mice homozygous for the targeted allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No transcript is detected in splenocytes or peritoneal macrophages. Thioglycollate-elicited peritoneal macrophages from homozygotes exhibit a delayed period before reaching peak mobilization (72 hours vs. 36 for wild type mice).

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain 5427. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A targeting vector containing a neomycin resistance gene was used to disrupt the single coding exon of the targeted gene. The construct was electroporated into 129P2/OlaHsd-derived E14TG2A embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts to obtain chimeric animals. Mutant mice were maintained on a mixed C57BL/6 and 129P2 background for a period of time before they were donated to the Jackson Laboratory Induced Mutant Resource (IMR) by Dr. William Kuziel. Subsequently they were obtained by Dr. Israel Charo (Gladstone Institute of Cardiovascular Disease, UCSF) and backcrossed to C57BL/6 ten times before being imported once more into the IMR. The mice were crossed to C57BL/6J once upon arrival.

Allele

Symbol: Ccr5^tm1Kuz
Name: targeted mutation 1, William A Kuziel
MGI accession ID: MGI:1861911
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Ccr5
Marker name: chemokine (C-C motif) receptor 5
Chromosome: 9
Strain of origin: 129P2/OlaHsd
Molecular note: The exon containing the entire coding region was replaced with a neomycin resistance gene inserted by homologous recombination.