Absence of chemokine (C-C motif) receptor 5 in these knock-out mice affects immune and a number of other physiological responses. They are useful for widespread studies of innate immunity.
Israel F. Charo, Gladstone Inst of Cardiovascular Disease, UCSF
At birth, mice homozygous for the targeted allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No transcript is detected in splenocytes or peritoneal macrophages. Thioglycollate-elicited peritoneal macrophages from homozygotes exhibit a delayed period before reaching peak mobilization (72 hours vs. 36 for wild type mice).
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain 5427. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector containing a neomycin resistance gene was used to disrupt the single coding exon of the targeted gene. The construct was electroporated into 129P2/OlaHsd-derived E14TG2A embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts to obtain chimeric animals. Mutant mice were maintained on a mixed C57BL/6 and 129P2 background for a period of time before they were donated to the Jackson Laboratory Induced Mutant Resource (IMR) by Dr. William Kuziel. Subsequently they were obtained by Dr. Israel Charo (Gladstone Institute of Cardiovascular Disease, UCSF) and backcrossed to C57BL/6 ten times before being imported once more into the IMR. The mice were crossed to C57BL/6J once upon arrival.
|Allele Name||targeted mutation 1, William A Kuziel|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||CCR5-; CCR5 KO; Cmkbr5tm1Kuz|
|Gene Symbol and Name||Ccr5, chemokine (C-C motif) receptor 5|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||The exon containing the entire coding region was replaced with a neomycin resistance gene inserted by homologous recombination.|
|Mutations Made By|| |
Dr. William Kuziel, Protein Design Laboratories
When maintaining a live colony, these mice are bred as homozygotes.
When using the CCR5 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #005427 in your Materials and Methods section.