These mice carry Chromosome 7 alleles from strain 129S1/SvImJ at the Ins2 loci and are useful as controls for congenic strain NOD.129S2(B6)-Ins2tm1Jja/GseJ in studies relating to diabetes.
George Eisenbarth, U of Colorado
|Marker Symbol||Marker Name|
|D7Mit105||DNA segment, Chr 7, Massachusetts Institute of Technology 105|
|D7Mit223||DNA segment, Chr 7, Massachusetts Institute of Technology 223|
This NOD/ShiLt congenic strain, commonly called NOD.Ins2 control, is carrying Chromosome 7 alleles derived from strain 129S1/SvImJ at the Ins2 loci. Diabetes onset and incidence among NOD.129-(D7Mit105 -D7Mit223)/GseJ congenic mice is similar to NOD. (Nakayama M, Nature 2005 435:220-3)
This strain is useful as a control for the exploration of Ins2 in the context of the NOD congenic Ins2 targeted mutation (Stock No. 005036).
A Chr. 7 genomic segment extending from D7Mit105 (63.5cM) through D7Mit223 (72.4cM) that includes the Ins2 loci (69.1cM) was transferred from 129S1/SvImJ to NOD/Bdc using speed congenic techniques (Nakayama M, Nature 2005 435:220-3). In 2005, the Type 1 Diabetes Resource (T1DR) received heterozygous mice at N8. Mice carrying the D7Mit105 (63.5cM) - D7Mit223 (72.4cM) segment from 129S1/SVImJ were intercrossed to make a homozygous strain. Additional microsatellite analysis confirms all known Idd alleles (1-20) are NOD/Bdc in origin.
A footpad injection of Complete Freund s Adjuvant (CFA) administered once at weaning will delay diabetes onset, thus extending the lifespan of breeders. Use of Complete Freund s Adjuvant in NOD mice can be found in Current Protocols in Immunology page 15.9.19, Reproduction.
When using the NOD.Ins2129 control mouse strain in a publication, please cite the originating article(s) and include JAX stock #005353 in your Materials and Methods section.