ALR/Lt-mt^NOD/ShiLtDvs/MxLt

Stock number: 005333
Product name: ALR.mt(NOD)
Research areas: Research Tools

Description

Mitochondria from this conplastic ALR.mt(NOD) strain produce more reactive oxygen species than mice from parental or reciprocal conplastic strains.

Detailed description

ALR mice are resistant to chemically induced and autoimmune type 1 diabetes and encode the mitochondrial mt-Nd2^a allele which is associated with resistance to type 1 diabetes. NOD mice are susceptible to type 1 diabetes and encode the mt-Nd2^c allele. Although basal mitchondrial respiration is unchanged, mitochondria from this conplastic produce more reactive oxygen species than either parent or the reciprocal conplastic.

Development

Dr. Clayton Mathews developed the ALR/Lt-mt^NOD/LtDvs/MxLt conplastic strain by crossing ALR/Lt males to the donor NOD/LtDvs females. F1 progeny from the cross carry NOD/LtDvs mitochondrial DNA. Female progeny were then sequentially backcrossed for 10 generations to ALR/Lt males . A conplastic strain carries the mitochondrial genome of the donor strain.