These mice carry an ENU-induced mutation at the Notch3 locus and exhibit myopathy.
Read More +Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Chemically induced (ENU) | Notch3 | notch 3 |
Mice homozygous for the humpback mutation appear normal at wean age, but by 5 weeks of age can be distinguished by thoracic kyphosis and scrawniness. Both phenotypes progress with age, and sudden death is common in adults. Histology reveals myopathy. Homozygotes females are not able to deliver pups and homozygous males develop phimosis so do not breed.
The hpbk mutation was identified in the great-granddaughter of an N-ethyl-N-nutrosourea (ENU) mutagenized C57BL/6J male in the Neuromutagenesis Facility at The Jackson Laboratory in 2001. A C57BL/6J male was treated with ENU and crossed to an untreated C57BL/6J female. The male offspring were bred with C57BL/6J females and their female offspring were backcrossed to their mutation-bearing father. The recessive mutations were revealed in the offspring and included one female with a humped spine. The ovaries of this mutant female were transplanted into a host and she was bred with a C57BL/6J male from which the heterozygous offspring were intercross to generate more humpback homozygotes. This mutant strain has been maintained by breeding the host of homozygous ovarian transplant with unaffected male siblings (heterozygotes or wild-type) and the heterozygous offspring are then sibling intercrossed.
Allele Name | humpback |
---|---|
Allele Type | Chemically induced (ENU) |
Allele Synonym(s) | |
Gene Symbol and Name | Notch3, notch 3 |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 17 |
Molecular Note | Exome sequencing of this ENU induced mutation revealed a G-to-A point mutation in Notch3, which disrupts the splice donor site at the exon 31-intron 31 boundary, changing it from G-GT to G-AT. |
When using the humpback mouse strain in a publication, please cite the originating article(s) and include JAX stock #005330 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for hpbk |
Frozen Mouse Embryo | C57BL/6J-Notch3<hpbk>/GrsrJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Notch3<hpbk>/GrsrJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Notch3<hpbk>/GrsrJ Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | C57BL/6J-Notch3<hpbk>/GrsrJ Frozen Embryos | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.