129.Cg-Ppia^tm1Lubn/J

Stock number: 005320
Product name: Ppia-
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These Ppia^tm1Lubn knock-out mice develop allergic pathology with elevated Th2 cytokine production.

Detailed description

Mice that are homozygous for the targeted mutation are viable, normal in size, and do not display any gross behavioral abnormalities. Homozygotes have reduced fertility. No gene product (protein) is detected by Western blot analysis. Approximately one third of homozygotes spontaneously develop blepharitis (inflammation of the eyelids). Histological examination reveals mononuclear cell, eosinophil and mast cell infiltration of the eyelid. Homozygotes exhibit splenomegaly and elevated levels of total serum IfG1 and IgE. CD4+ T cells isolated from homozygous mice have increased production of IL-2 (3 to 5 times normal) and IL-4. Cultured effector/memory CD4+ T cells are hypersensitive to TCR stimulation and have elevated Th2 cytokine production. This mutant mouse strain may be useful in studies of allergic reactions and effects of cyclosporine on signal transduction.

Development

A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exons 1 through 4 and part of exon 5. The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into MF1 blastocysts. The resulting chimeric animals were crossed to MF1 outbred mice, and then backcrossed to 129S6/SvEvTac for 20 generations.

Allele

Symbol: Ppia^tm1Lubn
Name: targeted mutation 1, Jeremy Luban
MGI accession ID: MGI:3505553
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Ppia
Marker name: peptidylprolyl isomerase A
Chromosome: 11
Strain of origin: 129P2/OlaHsd
Molecular note: A neomycin resistance gene replaced all but the last 60 bp of the coding sequence. Immunoblot analysis confirmed absence of protein in thymus, spleen, kidney, and brain.