Mice that are homozygous for the targeted mutation are viable, normal in size, and do not display any gross behavioral abnormalities. Homozygotes have reduced fertility. No gene product (protein) is detected by Western blot analysis. Approximately one third of homozygotes spontaneously develop blepharitis (inflammation of the eyelids). Histological examination reveals mononuclear cell, eosinophil and mast cell infiltration of the eyelid. Homozygotes exhibit splenomegaly and elevated levels of total serum IfG1 and IgE. CD4+ T cells isolated from homozygous mice have increased production of IL-2 (3 to 5 times normal) and IL-4. Cultured effector/memory CD4+ T cells are hypersensitive to TCR stimulation and have elevated Th2 cytokine production. This mutant mouse strain may be useful in studies of allergic reactions and effects of cyclosporine on signal transduction.

These <i>Ppia<sup>tm1Lubn</sup></i> knock-out mice develop allergic pathology with elevated Th2 cytokine production.

Typically mice are recovered in 12-16 weeks. Contact Customer Service to place an order or for more information.

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