These Ppiatm1Lubn knock-out mice develop allergic pathology with elevated Th2 cytokine production.
Jeremy Luban, University of Massachusetts Medical School
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Ppia | peptidylprolyl isomerase A |
Mice that are homozygous for the targeted mutation are viable, normal in size, and do not display any gross behavioral abnormalities. Homozygotes have reduced fertility. No gene product (protein) is detected by Western blot analysis. Approximately one third of homozygotes spontaneously develop blepharitis (inflammation of the eyelids). Histological examination reveals mononuclear cell, eosinophil and mast cell infiltration of the eyelid. Homozygotes exhibit splenomegaly and elevated levels of total serum IfG1 and IgE. CD4+ T cells isolated from homozygous mice have increased production of IL-2 (3 to 5 times normal) and IL-4. Cultured effector/memory CD4+ T cells are hypersensitive to TCR stimulation and have elevated Th2 cytokine production. This mutant mouse strain may be useful in studies of allergic reactions and effects of cyclosporine on signal transduction.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exons 1 through 4 and part of exon 5. The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into MF1 blastocysts. The resulting chimeric animals were crossed to MF1 outbred mice, and then backcrossed to 129S6/SvEvTac for 20 generations.
Allele Name | targeted mutation 1, Jeremy Luban |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | CypA-; Ppia- |
Gene Symbol and Name | Ppia, peptidylprolyl isomerase A |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 11 |
Molecular Note | A neomycin resistance gene replaced all but the last 60 bp of the coding sequence. Immunoblot analysis confirmed absence of protein in thymus, spleen, kidney, and brain. |
Mutations Made By | Jeremy Luban, University of Massachusetts Medical School |
The strain is maintained as a heterozygote due to reduced fertility of homozygotes.
When using the Ppia- mouse strain in a publication, please cite the originating article(s) and include JAX stock #005320 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Ppia<tm1Lubn> |
Frozen Mouse Embryo | 129.Cg-Ppia<tm1Lubn>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | 129.Cg-Ppia<tm1Lubn>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | 129.Cg-Ppia<tm1Lubn>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | 129.Cg-Ppia<tm1Lubn>/J Frozen Embryos | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.