B6.Cg-Tg(TcraY1,TcrbY1)416Tev/J

Stock number: 005236
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These mice express a transgene containing a rearranged T cell receptor transgene specific for the H2-D^b-restricted SV40 large tumor antigen epitope I.

Detailed description

This transgenic strain carries a rearranged T cell receptor transgene specific for the H2-D^b-restricted SV40 large tumor antigen epitope I (residues 206-215). Flow cytometric analysis of lymphocytes detects approximately 94% of CD8+ T cells are specific for the tumor antigen. Mice that are homozygous for the transgenic insert are viable, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of tumor immunology and peripheral T cell tolerance.

Development

Transgenic constructs containing the alpha-chain and beta-chain of the T cell receptor from the SV40 large tumor antigen epitope I-specific cytoxic T lymphocyte clone Y-1 were coinjected into fertilized B6D2F1/J mouse eggs. Founder line 416 animals were backcrossed to C57BL/6 mice for 13 generations.

Allele

Symbol: Tg(TcraY1,TcrbY1)416Tev
Name: transgene insertion 416, Satvir S Tevethia
MGI accession ID: MGI:3700739
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(TcraY1,TcrbY1)416Tev
Marker name: transgene insertion 416, Satvir S Tevethia
Chromosome: UN
Strain of origin: (C57BL/6J x DBA/2J)F1/J
Expressed genes: Tcra,T cell receptor alpha chain,mouse, laboratory; Tcrb,T cell receptor beta chain,mouse, laboratory
Molecular note: Single-cell embryos were coinjected with T-cell receptor alpha and T-cell receptor beta expression vectors containing the rearranged genomic Tcra and Tcrb VJ/V(D)J segments from the cytotoxic T-lymphocyte clone Y-1, which expresses a T cell receptor specific for the H2-D^b-restricted SV40 large tumor antigen epitope I (amino acids 206-215).
General note: Transgenic mouse lines were derived from 2 transgene-expressing founder mice and designated lines 416 and 422. The Tg insert of line 416 was backcrossed onto C57BL/6 to create a congenic strain for use in experiments.