B6.D2-(D8Mit256-D8Mit42)/LusJ

Stock number: 005173
Product name: B6.D2.8PM
Research areas: Research Tools

Description

These congenic genome-tagged mice carry a Chromosome 8 segment between markers Mit256 to Mit42 from the DBA/2 strain.

Detailed description

This C57BL/6J congenic carries the proximal middle portion of chromosome 8 from DBA/2J. The Mit or SNP (rs) markers in the strain name define the congenic interval.

Marker-assisted breeding was used to construct a set of overlapping congenic strains called genome-tagged mice (GTM) that span the entire mouse genome. Each member of the panel carries an average of a 23 cM segment (range 8 to 58 cM) from DBA/2J introgressed on to a C57BL/6J background. The segments represent the distal (D), middle (M), and proximal (P) portions of all or most of a given chromosome. (Iakoubova OA, et. al., 2001) GTM are a valuable resource for mapping and confirmation of loci contributing to complex traits.

The Jackson Laboratory is working with Dr. Lusis' group to import and make available additional strains in the panel.

Development

This congenic panel was initiated by a cross of B6D2F1/J (Stock #100006) females to C57BL/6J males. Male progeny at each generation were selected by marker assisted genome scan and were backcrossed to C57BL/6J females to produce the N3-N6 generations. All animals in the panel carry a C57BL/6J Y chromosome. Sibling pairs at the N6 generation were intercrossed to produce homozygotes. Iakoubova OA, et. al. provides a more detailed explanation of the chromosome intervals. Dr. Lusis donated the panel to The Jackson Laboratory in 2004.

Allele

MGI accession ID: MGI:100154
Marker symbol: D8Mit256
Marker name: DNA segment, Chr 8, Massachusetts Institute of Technology 256
Chromosome: 8

Allele

MGI accession ID: MGI:94407
Marker symbol: D8Mit42
Marker name: DNA segment, Chr 8, Massachusetts Institute of Technology 42
Chromosome: 8