This strain serves as a Cre reporter strain. When used in conjunction with a Cre recombinase-expressing strain, successful Cre-mediated excision is indicated by luciferase expression in Cre-expressing tissues.
This allele is also available on the C57BL/6 genetic background as Stock No. 034320.
William G. Kaelin, Dana Farber Cancer Institute
Genetic Background | Generation |
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N4+N3F11
|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), Reporter) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
These mice contain the firefly luciferase (luc) gene inserted into the Gt(ROSA)26Sor locus. Expression of the luciferase gene is blocked by a loxP-flanked STOP fragment placed between the luc sequence and the Gt(ROSA)26Sor promoter. This strain serves as a Cre reporter strain. When used in conjunction with a Cre recombinase-expressing strain, successful Cre-mediated excision is indicated by luciferase expression in Cre-expressing tissues. Mice that are homozygous for the transgenic insert are viable and fertile.
This allele is also available on the C57BL/6 genetic background as Stock No. 034320.
A targeting vector containing a PGK-neo cassette and a loxP-stop-loxP cassette upstream of the firefly luciferase (luc) gene was inserted into the Gt(ROSA)26Sor locus. The construct was introduced into 129S6/SvEvTac-derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were bred to FVB mice. The resulting mutant colony was backcrossed four generations onto the FVB genetic background and then sent to The Jackson Laboratory in 2005. As of December 2019, the live colony has been backcrossed to FVB/NJ three additional generations.
Expressed Gene | luc, luciferase, firefly |
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Site of Expression | when crossed to a cre recombinase-expressing strain, luciferase expression is observed in the cre-expressing tissues |
Allele Name | targeted mutation 1, William G Kaelin |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter) |
Allele Synonym(s) | Gt(ROSA)26Sortm1(Luc)Kaelin; ROSA26 L-S-L-Luc; ROSA26-Fluc |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | luc, luciferase, firefly |
Site of Expression | when crossed to a cre recombinase-expressing strain, luciferase expression is observed in the cre-expressing tissues |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 6 |
Molecular Note | The endogenous locus is interrupted by a construct comprising a loxP site, a neomycin resistance cassette with a strong, triple SV40 polyadenylation signal, and a second loxP site in the same orientation as the first followed by the cDNA sequence encoding firefly luciferase and the bovine growth hormone polyadenylation signal. In cells in which cre recombinase is expressed, the DNA between the loxP sites is excised, along with one loxP site, permitting expression of the luciferase gene under control of the ubiquitous ROSA26 promoter. |
Mutations Made By | Michal Safran, Dana Farber Cancer Institute |
This strain is maintained as a homozygote.
When using the FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #005125 in your Materials and Methods section.
Service/Product | Description | Price |
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Heterozygous or Wild-type for Gt(ROSA)26Sor<tm1(Luc)Kael> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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