FVB.129S6(B6)-Gt(ROSA)26Sor^tm1(Luc)Kael/J

Stock number: 005125
Research areas: Research Tools

Description

This strain serves as a Cre reporter strain. When used in conjunction with a Cre recombinase-expressing strain, successful Cre-mediated excision is indicated by luciferase expression in Cre-expressing tissues.

This allele is also available on a STOCK (mixed) background as Stock No. 034320.

Detailed description

These mice contain the firefly luciferase (luc) gene inserted into the Gt(ROSA)26Sor locus. Expression of the luciferase gene is blocked by a loxP-flanked STOP fragment placed between the luc sequence and the Gt(ROSA)26Sor promoter. This strain serves as a Cre reporter strain. When used in conjunction with a Cre recombinase-expressing strain, successful Cre-mediated excision is indicated by luciferase expression in Cre-expressing tissues. Mice that are homozygous for the transgenic insert are viable and fertile.

This allele is also available on a STOCK (mixed) genetic background as Stock No. 034320.

Development

A targeting vector containing a PGK-neo cassette and a loxP-stop-loxP cassette upstream of the firefly luciferase (luc) gene was inserted into the Gt(ROSA)26Sor locus. The construct was introduced into 129S6/SvEvTac-derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were bred to FVB mice. The resulting mutant colony was backcrossed four generations onto the FVB genetic background and then sent to The Jackson Laboratory in 2005. As of December 2019, the live colony has been backcrossed to FVB/NJ three additional generations.

Allele

Symbol: Gt(ROSA)26Sor^tm1(Luc)Kael
Name: targeted mutation 1, William G Kaelin
MGI accession ID: MGI:3052356
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); Reporter
Marker symbol: Gt(ROSA)26Sor
Marker name: gene trap ROSA 26, Philippe Soriano
Chromosome: 6
Strain of origin: 129S6/SvEvTac
Expressed genes: luc,luciferase,firefly
Site of expression: Cre recombinase inducible expression of luciferase
Molecular note: The endogenous locus is interrupted by a construct comprising a loxP site, a neomycin resistance cassette with a strong, triple SV40 polyadenylation signal, and a second loxP site in the same orientation as the first followed by the cDNA sequence encoding firefly luciferase and the bovine growth hormone polyadenylation signal. In cells in which cre recombinase is expressed, the DNA between the loxP sites is excised, along with one loxP site, permitting expression of the luciferase gene under control of the ubiquitous ROSA26 promoter.