129S(FVB)-Men1^tm1.2Ctre/J

Stock number: 005109
Product name: Men1dN/dN
Research areas: Research Tools

Description

These floxed mutant mice possess loxP sites flanking exons 3 to 8 of the Men1 gene. This strain may be useful for generating conditional mutations in applications related to studies of hypercalemia and parathyroid neoplasia.

Detailed description

These mice possess loxP sites flanking exons 3 to 8 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

When bred to a strain expressing Cre recombinase in parathyroid tissue (see Stock No. 005989 for example), this mutant mouse strain may be useful in studies of hypercalemia and parathyroid neoplasia.

Development

A loxP site flanked targeting vector containing a loxP-bracketed neomycin resistance gene under the control of the mouse phosphoglycerate kinase promoter was inserted into intron 2 of the Men1 gene. Another loxP site was positioned within intron 8. The construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric male animals were bred to 129S6/SvEvTacFBR females. The progeny were heterozygous for the floxed allele (TSM/+). These heterozygous mice were bred with a ubiquitously expressing cre mouse line (Ella-cre) on an FVB background. The resulting mice were heterozygous for an allele that lacked the PGK-neo cassette but contained loxP sites in introns 2 and 8, flanking exons 3 to 8.

Allele

Symbol: Men1^tm1.2Ctre
Name: targeted mutation 1.2, Judy S Crabtree
MGI accession ID: MGI:2683274
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Men1
Marker name: multiple endocrine neoplasia 1
Chromosome: 19
Strain of origin: 129S6/SvEvTac
Molecular note: Exons 3 through 8 are flanked by single loxP sites in introns 2 and 8. This allele was generated by removing the floxed neo cassette from Men1^tm1Ctre via cre-mediated recombination.
General note: Phenotypic Similarity to Human Syndrome: Hyperparathyroidism J:86642.