Homozygous mice have a reduced number of CD44 high CD4+ splenic T-cells, and delayed-type hypersensitivity (DTH) response (swelling) is impaired. In vitro T cell proliferation, IFN-gamma and IL2 production is diminished. This mutant mouse strain may be useful in studies of delayed-type hypersensitivity and inflammatory responses.
Theodore M Danoff, University of Pennsylvania
Genetic Background | Generation |
---|---|
N9+N3F13
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Ccl5 | chemokine (C-C motif) ligand 5 |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by Northern blot analysis of lipopolysaccharide (LPS) stimulated peritoneal exudates cells. Homozygous mice have a reduced number of CD44 high CD4+ splenic T-cells. Delayed-type hypersensitivity (DTH) response (swelling) is impaired in homozygous mice sensitized with subcutaneous injection of keyhole limpet hemocyanin or bovine purified protein derivative. Immunohistochemical analysis of the DTH treated footpads reveals that the numbers of infiltrating macrophages is decreased, while infiltrating CD4+ and CD8+ T cells numbers remain unchanged. In vitro T cell proliferation, IFN-gamma and IL2 production is diminished. This mutant mouse strain may be useful in studies of delayed-type hypersensitivity and inflammatory responses.
A targeting vector containing a hygromycin resistance gene driven by the phosphoglycerate kinase promoter and a diphtheria toxin gene was used to disrupt 334 bp of sequence in exon I. The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6 for 9 generations (June 2004).
Allele Name | targeted mutation 1, Hiroshi Sato |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | CCL5-KO; RANTES- |
Gene Symbol and Name | Ccl5, chemokine (C-C motif) ligand 5 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 11 |
Molecular Note | A 333 bp region of the gene containing the promoter, transcription and translation initiation sites, and part of exon 1 was replaced with a PGK-hygro cassette via homologous recombination. Northern blot analysis confirmed the absence of gene expression in homozygous mutant mice. |
Mutations Made By | Donald Cook, NIH/NIEHS |
When maintaining a live colony, these mice are bred as homozygotes.
When using the RANTES KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #005090 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Ccl5<tm1Hso> |
Frozen Mouse Embryo | B6.129P2-Ccl5<tm1Hso>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Ccl5<tm1Hso>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Ccl5<tm1Hso>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129P2-Ccl5<tm1Hso>/J Frozen Embryos | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.