Homozygous mice have a reduced number of CD44 high CD4+ splenic T-cells, and delayed-type hypersensitivity (DTH) response (swelling) is impaired. In vitro T cell proliferation, IFN-gamma and IL2 production is diminished. This mutant mouse strain may be useful in studies of delayed-type hypersensitivity and inflammatory responses.
Theodore M Danoff, University of Pennsylvania
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by Northern blot analysis of lipopolysaccharide (LPS) stimulated peritoneal exudates cells. Homozygous mice have a reduced number of CD44 high CD4+ splenic T-cells. Delayed-type hypersensitivity (DTH) response (swelling) is impaired in homozygous mice sensitized with subcutaneous injection of keyhole limpet hemocyanin or bovine purified protein derivative. Immunohistochemical analysis of the DTH treated footpads reveals that the numbers of infiltrating macrophages is decreased, while infiltrating CD4+ and CD8+ T cells numbers remain unchanged. In vitro T cell proliferation, IFN-gamma and IL2 production is diminished. This mutant mouse strain may be useful in studies of delayed-type hypersensitivity and inflammatory responses.
A targeting vector containing a hygromycin resistance gene driven by the phosphoglycerate kinase promoter and a diphtheria toxin gene was used to disrupt 334 bp of sequence in exon I. The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6 for 9 generations (June 2004).
|Allele Name||targeted mutation 1, Hiroshi Sato|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||CCL5-KO; RANTES-|
|Gene Symbol and Name||Ccl5, chemokine (C-C motif) ligand 5|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A 333 bp region of the gene containing the promoter, transcription and translation initiation sites, and part of exon 1 was replaced with a PGK-hygro cassette via homologous recombination. Northern blot analysis confirmed the absence of gene expression in homozygous mutant mice.|
|Mutations Made By|| |
Donald Cook, NIH/NIEHS
When maintaining a live colony, these mice are bred as homozygotes.
When using the RANTES KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #005090 in your Materials and Methods section.
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