These 2xIDE mice express a transgene containing human insulin-degrading enzyme and levels of soluble alpha beta-amyloid X-40 and soluble alpha beta-amyloid X-42 are reduced.
Dennis J. Selkoe, Brigham & Womens Hsp, Harvard Med Schl
These transgenic mice express human insulin-degrading enzyme (IDE) under the direction of the mouse calcium/calmodulin-dependent protein kinase II alpha (Camk2a) promoter. Transgene expression is approximately 2 fold relative to wildtype controls, as detected by quantitative Western blot analysis and protease activity assay. The levels of soluble alpha beta-amyloid X-40 and soluble alpha beta-amyloid X-42 are reduced in 2 to 3 month old transgenic mice. Mice that are hemizygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed to B6.Cg-Tg(PDGFB-APPSwInd)20Lms/2J (Stock No. 006293) mice, amyloid beta plaque burden in the resulting double transgenic mice is reduced by half. This mutant mouse strain may be useful in studies of Alzheimer's Disease, specifically the enhancement of amyloid beta-protein degradation.
A transgenic construct containing the human insulin-degrading enzyme, IDE, gene under the control of the mouse calcium/calmodulin-dependent protein kinase II alpha (Camk2a) promoter, and SV40 polyadenylation site sequence was injected into fertilized C57BL/6 oocytes. The resulting founder animals were crossed to C57BL/6 mice, and are maintained on the C57BL/6 background.
|Expressed Gene||IDE, insulin degrading enzyme, human|
|Site of Expression|
|Allele Name||transgene insertion 1, Dennis J Selkoe|
|Allele Type||Transgenic (Humanized sequence, Inserted expressed sequence)|
|Gene Symbol and Name||Tg(Camk2a-IDE)1Selk, transgene insertion 1, Dennis J Selkoe|
|Promoter||Camk2a, calcium/calmodulin-dependent protein kinase II alpha, mouse, laboratory|
|Expressed Gene||IDE, insulin degrading enzyme, human|
|Strain of Origin||C57BL/6|
|General Note||Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. The levels of soluble alpha beta-amyloid X-40 and soluble alpha beta-amyloid X-42 are reduced in 2 to 3 month old transgenic mice. |
In conjunction with Tg(PDGFB-APPSwInd)20Lms, transgenic mice develop only about half the amyloid beta plaque burden displayed by transgenic mice carrying Tg(PDGFB-APPSwInd)20Lms alone.
|Molecular Note||Transgenic mice express human insulin-degrading enzyme (IDE) under the direction of the mouse calcium/calmodulin-dependent protein kinase II alpha (Camk2a) promoter. Transgene expression is approximately 2 fold relative to wildtype controls as detected by quantitative Western blot analysis and protease activity assay. The levels of soluble alpha beta-amyloid X-40 and soluble alpha beta-amyloid X-42 are reduced in 2 to 3 month old transgenic mice.|
|Mutations Made By|| |
Malcolm Leissring, Brigham & Womens Hsp, Harvard Med Schl
The strain is maintained as a hemizygote. The Donating Investigator reports reproductive performance of this strain is poor.
When using the 2xIDE mouse strain in a publication, please cite the originating article(s) and include JAX stock #005087 in your Materials and Methods section.
|Hemizygous or Non carrier for Tg(Camk2a-IDE)1Selk|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
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