C57BL/6-Tg(Camk2a-MME)3Selk/J

Stock number: 005086
Product name: 8xNEP
Research areas: Neurobiology Research

Description

These 8xNEP mice express a transgene containing human membrane metallo-endopeptidase and levels of soluble alpha beta-amyloid X-40 and soluble alpha beta-amyloid X-42 are reduced.

Detailed description

These transgenic mice express human membrane metallo-endopeptidase (MME) under the direction of the mouse calcium/calmodulin-dependent protein kinase II alpha (Camk2a) promoter. Transgene expression is approximately 8 fold relative to wildtype controls, as detected by quantitative Western blot analysis and protease activity assay. The levels of soluble alpha beta-amyloid X-40 and soluble alpha beta-amyloid X-42 are reduced in 2 to 3 month old transgenic mice. Mice that are hemizygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. Transgenic mice have poor reproductive performance. When crossed to B6.Cg-Tg(PDGFB-APPSwInd)20Lms/2J (Stock No. 006293) mice, amyloid beta plaques do not develop in the resulting double transgenic mice. This mutant mouse strain may be useful in studies of Alzheimer's Disease, specifically the enhancement of amyloid beta-protein degradation.

Development

A transgenic construct containing the human membrane metallo-endopeptidase, MME, gene under the control of the mouse calcium/calmodulin-dependent protein kinase II alpha (Camk2a) promoter, and SV40 polyadenylation site sequence was injected into fertilized C57BL/6 mouse oocytes. The resulting chimeric animals were crossed to C57BL/6 mice, and are maintained on the C57BL/6 background.

Allele

Symbol: Tg(Camk2a-MME)3Selk
Name: transgene insertion 3, Dennis J Selkoe
MGI accession ID: MGI:3043425
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(Camk2a-MME)3Selk
Marker name: transgene insertion 3, Dennis J Selkoe
Chromosome: UN
Strain of origin: C57BL/6
Expressed genes: MME,membrane metalloendopeptidase,human
Molecular note: Transgenic mice express human membrane metallo-endopeptidase (MME) under the direction of the mouse calcium/calmodulin-dependent protein kinase II alpha (Camk2a) promoter. Transgene expression is approximately 8 fold relative to wildtype controls, as detected by quantitative Western blot analysis and protease activity assay. The levels of soluble alpha beta-amyloid X-40 and soluble alpha beta-amyloid X-42 are reduced in 2 to 3 month old transgenic mice.
General note: Homozygous mutant mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities.

Transgenic mice have poor reproductive performance.

In conjunction with Tg(PDGFB-APPSwInd)20Lms, transgenic mice do not develop amyloid beta plaques.