These 8xNEP mice express a transgene containing human membrane metallo-endopeptidase and levels of soluble alpha beta-amyloid X-40 and soluble alpha beta-amyloid X-42 are reduced.
Dennis J. Selkoe, Brigham & Women¿s Hsp, Harvard Med Schl
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
These transgenic mice express human membrane metallo-endopeptidase (MME) under the direction of the mouse calcium/calmodulin-dependent protein kinase II alpha (Camk2a) promoter. Transgene expression is approximately 8 fold relative to wildtype controls, as detected by quantitative Western blot analysis and protease activity assay. The levels of soluble alpha beta-amyloid X-40 and soluble alpha beta-amyloid X-42 are reduced in 2 to 3 month old transgenic mice. Mice that are hemizygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. Transgenic mice have poor reproductive performance. When crossed to B6.Cg-Tg(PDGFB-APPSwInd)20Lms/2J (Stock No. 006293) mice, amyloid beta plaques do not develop in the resulting double transgenic mice. This mutant mouse strain may be useful in studies of Alzheimer's Disease, specifically the enhancement of amyloid beta-protein degradation.
A transgenic construct containing the human membrane metallo-endopeptidase, MME, gene under the control of the mouse calcium/calmodulin-dependent protein kinase II alpha (Camk2a) promoter, and SV40 polyadenylation site sequence was injected into fertilized C57BL/6 mouse oocytes. The resulting chimeric animals were crossed to C57BL/6 mice, and are maintained on the C57BL/6 background.
Expressed Gene | MME, membrane metalloendopeptidase, human |
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Site of Expression |
Allele Name | transgene insertion 3, Dennis J Selkoe |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | 8xNEP |
Gene Symbol and Name | Tg(Camk2a-MME)3Selk, transgene insertion 3, Dennis J Selkoe |
Gene Synonym(s) | |
Promoter | Camk2a, calcium/calmodulin-dependent protein kinase II alpha, mouse, laboratory |
Expressed Gene | MME, membrane metalloendopeptidase, human |
Strain of Origin | C57BL/6 |
Chromosome | UN |
General Note | Homozygous mutant mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Transgenic mice have poor reproductive performance. In conjunction with Tg(PDGFB-APPSwInd)20Lms, transgenic mice do not develop amyloid beta plaques. |
Molecular Note | Transgenic mice express human membrane metallo-endopeptidase (MME) under the direction of the mouse calcium/calmodulin-dependent protein kinase II alpha (Camk2a) promoter. Transgene expression is approximately 8 fold relative to wildtype controls, as detected by quantitative Western blot analysis and protease activity assay. The levels of soluble alpha beta-amyloid X-40 and soluble alpha beta-amyloid X-42 are reduced in 2 to 3 month old transgenic mice. |
Mutations Made By | Malcolm Leissring, Brigham & Women¿s Hsp, Harvard Med Schl |
When maintaining a live colony, this strain is maintained as a hemizygote. This strain has poor reproductive performance.
When using the 8xNEP mouse strain in a publication, please cite the originating article(s) and include JAX stock #005086 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Camk2a-MME)3Selk |
Frozen Mouse Embryo | C57BL/6-Tg(Camk2a-MME)3Selk/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Camk2a-MME)3Selk/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Camk2a-MME)3Selk/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Tg(Camk2a-MME)3Selk/J Frozen Embryos | $3373.50 |
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