NZL/LtJ

Stock number: 005067
Research areas: Diabetes and Obesity Research

Description

Male NZL/LtJ mice develop juvenile-onset obesity and maturity-onset hyperglycemia.

Detailed description

Over 90% of males exhibit hyperglycemia of >250 mg/dl by 20 weeks of age. Body weight in males increases to 55 gm by 16 weeks of age. The diabetic phenotype is very similar to that of NZO/HlLt J (Stock No. 002105) mice. An advantage of NZL/LtJ over NZO/HlLtJ as a diabetes model, in addition to the higher disease incidence, is that mice of this strain breed well, whereas NZO/HlLt mice breed very poorly.

This strain carries the wildtype Ighm allele from the NZO/HlLtJ strain.

Development

This strain was developed in parallel with NZL.129S2-Ighm^tm1Cgn/Lt. The Ighm^tm1Cgn targeted allele was obtained by Dr. Edward Leiter at N11 on the NZB/Hl background, to which it had been transferred from B6.129S2-Ighm^tm1Cgn. In Dr. Leiter's laboratory, the mutation was backcrossed onto the NZO/HlLt strain for 5 generations. The N5 Ighm^tm1Cgn heterozygotes were intercrossed, and two sublines were initiated from the progeny: Mice homozygous for the targeted mutation were selected to generate a homozygous Ighm^tm1Cgn line, and mice homozygous for the wildtype Ighm allele were chosen to start a parallel wildtype line.

As backcrossing was carried out for only 5 generations, the genome of these strains is, statistically, approximately 3.12% of NZB origin with known genetic regions on chromosones 9 and 18; there may also be minor contributions from C57BL/6 and 129S2/SvPas. The control line was found to have a severe diabetes phenotype comparable to that of the NZO/HlLt background strain, but to have certain advantages over the latter. For this reason, the new line was assigned its own inbred-strain nomenclature once it had reached the equivalent of 20 generations of sibling inbreeding.

This strain was donated to The Jackson Laboratory in 2004.