B6.129S2-Il10rb^tm1Agt/J

Stock number: 005027
Product name: CRFB4-
Research areas: Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research

Description

These Il10rb knock-out mice may be useful in studies of the role of interleukin-10 in inflammatory diseases such as chronic colitis.

Detailed description

Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by Northern blot analysis of ES cells. After 12 weeks of age, approximately 60% of mutant mice develop chronic colitis and increased numbers of splenocytes resulting in splenomegaly. Bone marrow-derived macrophages, splenocytes and peritoneal cells derived from homozygotes do not respond to IL-10. Mutant mice housed in non-SPF conditions do not breed well. This mutant mouse strain may be useful in studies of the role of interleukin-10 in inflammatory diseases such as chronic colitis.

Development

A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exons 1 and 2, which encode the signal peptide and the first 39 amino acids. The construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The donating investigator reported that the resulting male chimeric animals were crossed to C57BL/6 female mice, and then backcrossed to C57BL/6J mice for 6 generations using a speed congenic protocol (see SNP note below).

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Allele

Symbol: Il10rb^tm1Agt
Name: Targeted mutation 1, Michael Aguet
MGI accession ID: MGI:2182946
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Il10rb
Marker name: interleukin 10 receptor, beta
Chromosome: 16
Strain of origin: 129S2/SvPas
Molecular note: The first two exons, encoding 39 amino acids including the signal peptide, were replaced with a neomycin cassette. Northern blot analysis of homozygous mutant ES cells showed an absence of transcript.