These mice carry an ENU-induced mutation that is characterized by agouti dilute coat color, impaired hind limb movement, and instability.
The Jackson Laboratory cannot guarantee that cryorecovery of strains from the discontinued NIH-funded Neuroscience Mutagenesis Facility (NMF) will be successful or that the anticipated phenotype or genotype will be obtained. The cryorecovery fee for this effort will not be refunded or prorated if the recovery is unsuccessful or is in any way unsatisfactory. Genotyping will be the responsibility of the Purchaser.Read More +
The mutants are small with impaired hind limb movement, i.e. 'hot-foot' like behavior (of one or both limbs), and show agouti dilute coat color. These mice are fragile and usually die by or before 5 weeks of age; a colony needs to be maintained through ovarian transplants. Complementation tests between heterozygous NMF244 and DLS/LeJ mice produced six affected mice in a total of 22 progeny, suggesting that NMF244 represents a new allele of dilute-lethal in the Myo5a gene. These affected mice show a more severe phenotype than NMF244, i.e. they intermittently lean to the side, fall over, but are able to regain their walking position; they also show a non-agouti grey coat color. The coat color appears to result from a non-agouti grey allele that is known to exist in heterozygotes for the dilute-lethal allele of Myo5a, and becomes apparent in the presence of the agouti dilute allele, carried by the female NMF244. Standard pathology work-up on three mutants (23 - 29 days of age) revealed no abnormalities
This phenotypic deviant was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males (Stock No. 000664), in the Neuroscience Mutagenesis facility at The Jackson Laboratory.
|Allele Name||dilute lethal 31 Jackson|
|Allele Type||Chemically induced (ENU)|
|Allele Synonym(s)||Myo5anmf244; neuroscience mutagenesis facility, 244; NMF244|
|Gene Symbol and Name||Myo5a, myosin VA|
|Strain of Origin||A.B6-Tyr+|
|Molecular Note||This phenotypic mutant was identified in an ENU mutagenesis screen. Complementation tests between heterozygous NMF244 and DLS/LeJ mice produced six affected mice in a total of 22 progeny, suggesting that NMF244 represents a new allele of dilute-lethal in this gene.|