These mice carry an ENU-induced mutation that is characterized by an unsteady gait and occasional spasms culminating in popcorn like jumps.
The Jackson Laboratory cannot guarantee that cryorecovery of strains from the discontinued NIH-funded Neuroscience Mutagenesis Facility (NMF) will be successful or that the anticipated phenotype or genotype will be obtained. The cryorecovery fee for this effort will not be refunded or prorated if the recovery is unsuccessful or is in any way unsatisfactory. Genotyping will be the responsibility of the Purchaser.Read More +
The mutants exhibit an unsteady gait, at times staggering forward with their torso swaying intensely; hind limbs are splayed apart and show clumsy, overshooting movements. Occasionally (between 6-7 weeks of age), intense spasms can be observed in the hind limb and lower back area, which may culminate in high (pop-corn like) jumps. The phenotype becomes noticeable at approximately 4 weeks of age (mean 3.9+/- 1.4 weeks, n=96). Because of the phenotype and map position observed, complementation testing with Cacna1atg (JAX# 0544) was performed; however, heterozygote matings did not result in any mutants (16 normal progeny), and indicate therefore that nmf242 does not represent an allele of Cacna1a. Standard pathology work-up did not show any abnormalities.
A colony can be maintained through heterozygous or mutant by C57BL/6J matings.
This phenotypic deviant was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males (Stock No. 000664), in the Neuroscience Mutagenesis facility at The Jackson Laboratory. Mutagenized males were crossed to C57BL/6J females; G3 descendants of the mutagenized males were selected for neurological impairment.
|Allele Name||neuroscience mutagenesis facility, 242|
|Allele Type||Chemically induced (ENU)|
|Gene Symbol and Name||nmf242, neuroscience mutagenesis facility, 242|
|Strain of Origin||C57BL/6J|
|Molecular Note||This phenotypic mutation was discovered in an ENU mutagenesis screen.|