NOD/ShiLtDvs-Tg(Ins2-E3*734)4Dvs/DvsJ

Stock number: 004990
Product name: DL734/NOD4
Research areas: Diabetes and Obesity Research

Description

These DL734/NOD4 mice express a transgene containing adenovirus early region 3 (E3) sequences with two point mutations under direction of a rat insulin promoter and develop diabetes slower than control NOD mice.

Detailed description

Carriers of this transgene express the transcripts of the adenoviral E3 19K, 11.6K, 14.5K, 14.7K, and 10.4K glycoproteins in the islets but the adenovirus death protein is not expressed due to point mutations in the first two methionines of the 11.6K open reading frame. Female carriers of this transgene have slower kinetics and rates of development of diabetes through 30 weeks of age relative to NOD/Lt controls. These carriers do not display a significantly different diabetes rate than un-mutated adenovirus E3 carriers.

Allele

Symbol: Tg(Ins2-E3*734)4Dvs
Name: transgene insertion 4, David V Serreze
MGI accession ID: MGI:3785757
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Ins2-E3*734)4Dvs
Marker name: transgene insertion 4, David V Serreze
Chromosome: UN
Strain of origin: Not Applicable
Expressed genes: E3,adenovirus early region 3,adenovirus
Molecular note: The rat insulin promoter was placed upstream of the adenovirus early region 3 (E3) sequences with two point mutations inthe first two methionines of the 11.6K open reading frame.