NOD.B6-Itgb7^tm1Cgn/2LtJ

Stock number: 004944
Product name: beta7^-
Strain synonyms: NOD.Itgb7^null
Research areas: Cell Biology Research, Immunology, Inflammation and Autoimmunity Research

Description

These Itgb7 knock-out mice exhibit hypoplasia of the gut-associated lymphoid tissue.

Detailed description

Mice homozygous for the targeted mutation have a marked hypoplasia of the gut-associated lymphoid tissue(GALT). This deficit is apparently due to the failure of Itgb7 deficient lymphocytes to arrest and adhere in the GALT.

Development

This strain was derived from an initial cross between mice of strains NOD/Lt and C57BL/6-Itgb7^tm1Cgn (Stock No. 002965), followed by 10 backcrosses (to N11) to NOD/Lt of progeny selected for heterozygosity for the targeted allele. Heterozygous offspring of the final backcross were intercrossed to initiate a line homozygous for Itgb7^tm1Cgn, and the strain was then maintained by sibling inbreeding. Statistically, this strain's genome is approximately 99.95% of NOD/Lt origin. Marker assisted analysis of all known Idd loci (1-21) are of NOD origin. This stock was transferred from Ed Leiter's research colony into the T1DR via rederivation.

Allele

Symbol: Itgb7^tm1Cgn
Name: targeted mutation 1, University of Cologne
MGI accession ID: MGI:1861953
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Itgb7
Marker name: integrin beta 7
Chromosome: 15
Strain of origin: C57BL/6
Molecular note: A neomycin resistance cassette replaced part of exon 12 and all of exons 13 and 14, which encode the transmembrane and part of the extracellular domains.