These NOD.RIP-tTA mice express a transgene containing the tetracycline transactivator (tTA) that permits the inducible expression of genes in pancreatic beta cells.
Christophe Benoist, Joslin Diabetes Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Transactivator) |
Tg(Ins2-tTA)1Doi encodes the tetracycline regulatable transactivator (tTA) regulated by the rat insulin promoter (Ins2, commonly designated RIP), and is expressed in the pancreatic beta cells. Mice hemizygous for this transgenic insert are viable, fertile, normal in size, display normal NOD diabetes onset, but do not display any other gross physical or behavioral abnormalities. When transgenic mice are mated to a second transgenic strain carrying the target gene regulated by the tetO responsive elements, expression of the target gene is turned off by the tetracycline analog, doxycycline (dox). Dox can be administered orally in the food or water. This strain provides a Tet-Off tool that permits the inducible expression of genes in the pancreatic beta cells during various stages of diabetes development.
Christophe Benoist et al., injected the Ins2-tTA transgenic construct into (C57BL/6J x SJL)F1 oocytes and backcrossed the resulting Tg(Ins2-tTA)1Doi allele onto the NOD background for 10 generations. In 2003, N10 mice were intercrossed at The Jackson Laboratory, and are maintained Tg/? x Tg/?
Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli |
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Site of Expression | Expresses tTA in pancreas. |
Allele Name | transgene insertion 1, Christophe Benoist |
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Allele Type | Transgenic (Transactivator) |
Allele Synonym(s) | RIP-tTA |
Gene Symbol and Name | Tg(Ins2-tTA)1Doi, transgene insertion 1, Christophe Benoist |
Gene Synonym(s) | |
Promoter | Ins2, insulin 2, rat |
Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli |
Site of Expression | Expresses tTA in pancreas. |
Strain of Origin | (C57BL/6J x SJL)F1 |
Chromosome | UN |
Molecular Note | Tg(Ins2-tTA)1Doi encodes the tetracycline regulatable transactivator (tTA) regulated by the rat insulin promoter (Ins2) and is expressed in the pancreatic beta cells. |
Mutations Made By | Christophe Benoist, Joslin Diabetes Center |
When held in a live colony, this strain is maintained Tg/? X Tg/? as we are unable to determine hemizygotes from homozygotes, A footpad injection of Complete Freund s Adjuvant (CFA) administered once at weaning will delay diabetes onset, thus extending the lifespan of breeders. Use of Complete Freund s Adjuvant in NOD mice can be found in Current Protocols in Immunologypage 15.9.19, Reproduction.
When using the NOD.RIP-tTA mouse strain in a publication, please cite the originating article(s) and include JAX stock #004937 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Ins2-tTA)1Doi |
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