Ts(16C-tel)1Cje trisomic mice display decreased exploratory behavior and have spatial learning deficits detectable in the hidden platform and probe versions of the Morris water maze. These learning deficits are less severe than those of mice carrying Ts(1716)Dn (Ts65Dn, Stock No. 001924 or 005252) with a larger distal Chr 16 translocation. The degeneration of basal forebrain cholinergic neurons that is found in Ts65Dn trisomic mice has not been found by six months of age in mice carrying Ts(16C-tel)1Cje. Additionally, the male sterility found in Ts65Dn mice is not a phenotype associated with Ts(16C-tel)1Cje (Sago et al., 1998). Despite having fewer trisomic genes, Ts(16C-tel)1Cje carriers display much of the same craniofacial dysmorphology as that found in Ts(1716)Dn, which includes brachycephaly, reduced interorbital breadth, and smaller mandible (Richtsmeier et ...
Pde6brd1, the recessive retinal degeneration 1 mutation, is segregating in this colony. Animals that are homozygous for rd1 will be blind.
Ts(16C-tel)1Cje trisomic mice display decreased exploratory behavior and have spatial learning deficits detectable in the hidden platform and probe versions of the Morris water maze. These learning deficits are less severe than those of mice carrying Ts(1716)Dn (Ts65Dn, Stock No. 001924 or 005252) with a larger distal Chr 16 translocation. The degeneration of basal forebrain cholinergic neurons that is found in Ts65Dn trisomic mice has not been found by six months of age in mice carrying Ts(16C-tel)1Cje. Additionally, the male sterility found in Ts65Dn mice is not a phenotype associated with Ts(16C-tel)1Cje (Sago et al., 1998). Despite having fewer trisomic genes, Ts(16C-tel)1Cje carriers display much of the same craniofacial dysmorphology as that found in Ts(1716)Dn, which includes brachycephaly, reduced interorbital breadth, and smaller mandible (Richtsmeier et al., 2002).
The Ts(16C-tel)1Cje trisomy was derived from a chromosomal translocation that was identified in the Laboratory of Dr. Charles Epstein during the screening that followed the creation of the tm1Cje targeted mutation of Sod1 on Chr 16. This targeted disruption was done in CB1-4 ES cells which were derived from the embryos of a C57BL/6J female bred to an (Rb(11.16)2H x Rb(16.17)32Lub) F1 male, and the founders were backcrossed to CD1 for several generations. In these progeny, a sub-population of mice was found that carried the neomycin resistance gene indicative of the targeted disruption of Sod1 but the mice had normal levels of copper-zinc superoxide dismutase activity. FISH analysis revealed an Sod1tm1Cje-bearing translocation of distal Chr 16 onto Chr 12, which was named T(12;16)1Cje (Stock No. 004838). By breeding translocation carriers to wild-type mice, a partial trisomy line (designated Ts(16C-tel)1Cje) was generated harboring two wild-type Chr 16 copies and an additional copy of distal Chr 16 translocated onto Chr 12. Further analysis also reveals a small deletion of the telomeric part of Chr 12, with a translocation breakpoint between positions 119,278,499 and 119,289,499, in the Dnahc11 gene, between exons 35 and 41. Approximately half of this gene is truncated, leading to a monosomy that encompasses all the genes located downstream toward the telomeric end of mouse Chr 12.
The Ts(16C-tel)1Cje strain was then backcrossed onto C57BL/6J by the Donating Investigator. In 1997, N10 carriers were sent to The Jackson Laboratory where they were backcrossed to C57BL/6J for 9 generations and then to C57BL/6JEiJ (003645) for several more generations. However, viability on the inbred background was severely reduced. Therefore, it was bred to B6EiC3SnF1/J (001875) mice henceforth to produce the current Ts(16C-tel)1Cje strain (004861), which has a genetic background comparable to that of the Ts65Dn trisomic strains (001924 and 005252).
In 2007, embryos were generated for cryopreservation via in vitro fertilization using B6EiC3SnF1/J females and trisomic males.
|Allele Name||trisomy, (16C-tel), 1 Charles J Epstein|
|Allele Synonym(s)||Ts(1216)1Cje; Ts108Cje; Ts1Cje; partial trisomy 16|
|Gene Symbol and Name||Ts(16C-tel)1Cje, trisomy, (16C-tel), 1 Charles J Epstein|
|Gene Synonym(s)||Ts(1216)1Cje; Ts(1216)1Cje; Ts108Cje; Ts1Cje; partial trisomy 16; trisomy,Chr 12 translocation to Chr 16, Charles J Epstein|
|Strain of Origin||C57BL/6J x (Rb(11.16)2H x Rb(16.17)32Lub)F1|
|Molecular Note||This chromosomal trisomy comprises two normal Chr 16s and the reciprocal translocation product 1216. Mice are trisomic for the cytogenetic interval 16C-tel and the genetic interval Sod1 to Mx1. Sod1 is not functionally trisomic because the Sod1 gene in the translocated segment is inactivated by the insertion of the neomycin resistance sequence. The Ts1Cje mouse model ofDown syndrome is trisomic for 94 genes on Mmu16, covering approximately two-thirds of the trisomic region in Ts65Dn mice.|
|Please inquire about possible genotypes.|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in
order to provide the minimum number of animals, animals will ship within 25 weeks.
The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.
MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.
Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.