The Ts(16C-tel)1Cje trisomy was derived from a chromosomal translocation that was identified in the Laboratory of Dr. Charles Epstein during the screening that followed the creation of the tm1Cje targeted mutation of Sod1 on Chr 16. This targeted disruption was done in CB1-4 ES cells which were derived from the embryos of a C57BL/6J female bred to an (Rb(11.16)2H x Rb(16.17)32Lub) F1 male, and the founders were backcrossed to CD1 for several generations. In these progeny, a sub-population of mice was found that carried the neomycin resistance gene indicative of the targeted disruption of Sod1 but the mice had normal levels of copper-zinc superoxide dismutase activity. FISH analysis revealed an Sod1tm1Cje-bearing translocation of distal Chr 16 onto Chr 12, which was named T(12;16)1Cje (Stock No. 004838). By breeding translocation carriers to wild-type mice, a partial trisomy line (designated Ts(16C-tel)1Cje) was generated harboring two wild-type Chr 16 copies and an additional copy of distal Chr 16 translocated onto Chr 12. Further analysis also reveals a small deletion of the telomeric part of Chr 12, with a translocation breakpoint between positions 119,278,499 and 119,289,499, in the Dnahc11 gene, between exons 35 and 41. Approximately half of this gene is truncated, leading to a monosomy that encompasses all the genes located downstream toward the telomeric end of mouse Chr 12.
The Ts(16C-tel)1Cje strain was then backcrossed onto C57BL/6J by the Donating Investigator. In 1997, N10 carriers were sent to The Jackson Laboratory where they were backcrossed to C57BL/6J for 9 generations and then to C57BL/6JEiJ (003645) for several more generations. However, viability on the inbred background was severely reduced. Therefore, it was bred to B6EiC3SnF1/J (001875) mice henceforth to produce the current Ts(16C-tel)1Cje strain (004861), which has a genetic background comparable to that of the Ts65Dn trisomic strains (001924 and 005252).
In 2007, embryos were generated for cryopreservation via in vitro fertilization using B6EiC3SnF1/J females and trisomic males.
