These floxed mutant mice possess loxP sites flanking exons 2 and 3 of the Psen1 gene. This strain may be useful for generating conditional mutations in applications related to Alzheimer's disease.
Jie Shen, Harvard Med Sch/Brigham Women's Hosp
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Psen1 | presenilin 1 |
These mice possess loxP sites on either side of exons 2 and 3 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. Northern blot and RT-PCR analysis of brain tissue from homozygotes reveal gene product (mRNA) transcription levels and size are the same as wildtype. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 and 3 deleted in the cre-expressing tissues.
A loxP site flanked targeting vector containing hygromycin resistance and thymidine kinase genes under the control of the cytomegalovirus (CMV) promoter was utilized in the construction of this mutant. This selection cassette was inserted into intron 3 of the targeted gene, and another loxP site was inserted into intron 1. This construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells which were transiently transfected with a cytomegalovirus-driven Cre recombinase adenovirus vector to remove the selection cassette. ES cells in which Cre recombination resulted in exons 2 and 3 being flanked by loxP sites (the loxP1, 2/3 locus) were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to the same for 39 for generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Allele Name | targeted mutation 2, Jie Shen |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | fPS1; fPsen1; Presenilin 1 floxed; PS1C; Psen1f |
Gene Symbol and Name | Psen1, presenilin 1 |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 12 |
Molecular Note | A single loxP site was inserted into intron 1 and a loxP-flanked hygromycin and thymidine kinase selection cassette was inserted into intron 3. The drug selection cassette was removed in ES cells by transient Cre expression in ES cells prior to the production of mice. The final allele has single loxP sites flanking exons 2 and 3. |
Mutations Made By | Jie Shen, Harvard Med Sch/Brigham Women's Hosp |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.129S4-Psen1tm2Shn/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #004825 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Psen1<tm2Shn> |
Frozen Mouse Embryo | B6.129S4-Psen1<tm2Shn>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Psen1<tm2Shn>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Psen1<tm2Shn>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S4-Psen1<tm2Shn>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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