B6.129S4-Psen1^tm2Shn/J

Stock number: 004825
Research areas: Developmental Biology Research, Mouse/Human Gene Homologs, Neurobiology Research, Research Tools

Description

These floxed mutant mice possess loxP sites flanking exons 2 and 3 of the Psen1 gene. This strain may be useful for generating conditional mutations in applications related to Alzheimer's disease.

Detailed description

These mice possess loxP sites on either side of exons 2 and 3 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. Northern blot and RT-PCR analysis of brain tissue from homozygotes reveal gene product (mRNA) transcription levels and size are the same as wildtype. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 and 3 deleted in the cre-expressing tissues.

Development

A loxP site flanked targeting vector containing hygromycin resistance and thymidine kinase genes under the control of the cytomegalovirus (CMV) promoter was utilized in the construction of this mutant. This selection cassette was inserted into intron 3 of the targeted gene, and another loxP site was inserted into intron 1. This construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells which were transiently transfected with a cytomegalovirus-driven Cre recombinase adenovirus vector to remove the selection cassette. ES cells in which Cre recombination resulted in exons 2 and 3 being flanked by loxP sites (the loxP1, 2/3 locus) were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to the same for 39 for generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Psen1^tm2Shn
Name: targeted mutation 2, Jie Shen
MGI accession ID: MGI:2449304
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Psen1
Marker name: presenilin 1
Chromosome: 12
Strain of origin: 129S4/SvJae
Molecular note: A single loxP site was inserted into intron 1 and a loxP-flanked hygromycin and thymidine kinase selection cassette was inserted into intron 3. The drug selection cassette was removed in ES cells by transient Cre expression in ES cells prior to the production of mice. The final allele has single loxP sites flanking exons 2 and 3.