A C57BL/6 congenic version of this strain is available as Stock No. 017491.
These transgenic mice express Cre recombinase under the control of the mouse transgelin (smooth muscle protein 22-alpha) promoter. Cre recombinase activity is observed in vascular smooth muscle cells of hepatic and pulmonary arteries. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in vascular smooth muscle cells. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful in studies of vascular diseases such as atherosclerosis.
Dr. Joachim Herz, Univ of Texas Southwest Med Ctr Dallas
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse transgelin (smooth muscle protein 22-alpha) promoter. Cre recombinase expression (mRNA) closely patterns endogenous transgelin expression, with the highest levels detected in the aorta, intestine and uterus. Low levels of transcript are detected in all other organs tested, likely reflecting the vascular smooth muscle compartments in the these tissues. Cre recombinase activity is observed in vascular smooth muscle cells of hepatic and pulmonary arteries, with no activity detected outside the vascular walls. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in vascular smooth muscle cells. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful in studies of vascular diseases such as atherosclerosis.
A transgenic construct containing cre coding sequence under the control of the mouse transgelin promoter was introduced into B6SJLF2 donor oocytes.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | vascular smooth muscle cells |
Allele Name | transgene insertion 1, Joachim Herz |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | SM; SM22a-Cre; Sm22alphaCre; SM22-Cre; SM22-Cretg; Tag1-Cre; Tgln-Cre |
Gene Symbol and Name | Tg(Tagln-cre)1Her, transgene insertion 1, Joachim Herz |
Gene Synonym(s) | |
Promoter | Tagln, transgelin, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | vascular smooth muscle cells |
Strain of Origin | (C57BL/6 x SJL)F2 |
Chromosome | UN |
General Note | Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. |
Molecular Note | The transgene consists of a Tagln (transgelin) promoter and a cre gene modified to include translational consensus and nuclear localization signals. The Tagln promoter was used to drive transgene expression in smooth muscle cells. Cre recombinase mRNA expression closely patterns endogenous transgelin expression, with the highest levels detected in the aorta, intestine, and uterus. Low levels of Cre recombinase transcript are detected in all other organs. Recombination occurs in vascular smooth muscle cells of hepatic and pulmonary arteries. No recombination has been detected in non-muscle cells. |
Mutations Made By | Dr. Joachim Herz, Univ of Texas Southwest Med Ctr Dallas |
The strain is maintained on a mixed background of 129S5/SvEvBrd, C57BL6 and SJL. Expected coat color is black and agouti.
When using the STOCK Tg(Tagln-cre)1Her/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #004746 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Homozygous for Tg(Tagln-cre)1Her, 1 pair minimum |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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