STOCK Ednrb^s-52Pub

Stock number: 004711
Product name: 52Pub
Research areas: Dermatology Research, Developmental Biology Research, Research Tools

Detailed description

This strain represents one of a series of strains carrying overlapping chromosomal deficiencies surrounding the endothelin receptor B locus collected during the Oak Ridge specific-locus-test mutagenesis screen. These strains may be useful in genetic studies to dissect the functional content of a genomic region and several developmental defects.

Development

T stock is a tester stock homozygous for seven recessive alleles, a, Tyrp^b, Tyr^c-ch, p, Myo5a^d, Bmp5^se, and Ednrb^s. (Russell et al. 1979. Genetics v.91 p.127). (101/Rl x C3H/Rl)F1 males were mutagenized by exposure to plutonium-239 and bred with T stock Females to identify those carrying recessive mutations allelic with Ednrb^s. Proven heterozygotes were bred to CAST/Ei, which permits identification of the mutant versus CAST/Ei chromosomes through a D14Mit8 polymorphism. Ednrb^s-52Pub heterozygous male offspring were sent from Liane Russell at Oak Ridge National Laboratory to The Jackson Laboratory in July 1997 and bred with C57BL/6J females for re-derivation. In 2003 heterozygous males, from a cross of a heterozygote to BCByB6F1/J, were used for cryopreservation of sperm.

Allele

Symbol: Ednrb^s-52Pub
Name: Pu239 citrate alpha ray induced 52Pub
MGI accession ID: MGI:2155852
Generation method: Radiation induced
Marker symbol: Ednrb
Marker name: endothelin receptor type B
Chromosome: 14
Strain of origin: (101/Rl x C3H/Rl)F1
Molecular note: Large intergenic deletion.
General note: This allele was induced by [alpha-239]-Pu: Plutonium citrate (alpha particles).