Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous mice have an embryonic lethal phenotype, failing to develop past 11.5 days post coitum (d.p.c.). Placental formation is defective due to the failure of the chorion and the allantois to fuse. Morphological disorganization of the chorion is observed by 8.0-8.25 d.p.c. Integrin alpha 4 protein is not detectable in homozygous mutant chorionic cells by immunohistochemical analysis. This mutant mouse strain may be useful in studies related to the failure of chorion and allantois fusion during placental development.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt a portion of exon 3 and a portion of intron 3. The construct was electroporated into 129S1/Sv-p+ Tyr+ KitlSl-J/+ derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts.
|Allele Name||targeted mutation 1, Brian A Parr|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Wnt7b-; Wnt7bD3; Wnt7bD3-4|
|Gene Symbol and Name||Wnt7b, wingless-type MMTV integration site family, member 7B|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||The gene was disrupted by replacement of part of exon 3 and part of intron 3 with a neomycin resistance cassette via homologous recombination.|
|Mutations Made By|| |
Andrew McMahon, University of Southern California
The resulting chimeric animals were crossed to C57BL/6J mice. The strain is maintained as a heterozygote. Homozygotes are not viable.
When using the B6;129-Wnt7btm1Parr/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #004693 in your Materials and Methods section.