C.Cg-Tg(SKP2)4Paga/J

Stock number: 004687
Product name: CD4-SKP2
Research areas: Research Tools

Description

These CD4-SKP2 mice carry a transgene containing human SKP2 that is expressed in thymus and peripheral lymphoid organs under control of the mouse Cd4 promoter.

Detailed description

Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express human SKP2 under the direction of the mouse Cd4 minimal promoter/enhancer. Transgene expression occurs in thymus and peripheral lymphoid organs. This strain represents an effective tool for generating double transgenic mice that would be useful to study SKP2 interactions in carcinogenesis.

Development

A transgenic construct containing the human SKP2 gene, and the mouse Cd4 minimal promoter/enhancer was injected into fertilized B6D2F1 mouse oocytes. The resulting mice from founder line 4 were backcrossed for 10 generations on the BALB/c background.

Allele

Symbol: Tg(SKP2)4Paga
Name: transgene insertion 4, Michele Pagano
MGI accession ID: MGI:3057133
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(SKP2)4Paga
Marker name: transgene insertion 4, Michele Pagano
Chromosome: UN
Strain of origin: (C57BL/6 x DBA/2)F1
Expressed genes: SKP2,S-phase kinase associated protein 2,human
Molecular note: The transgene contains the human SKP2 gene and the mouse Cd4 minimal promoter/enhancer. Transgene expression occurs in thymus and peripheral lymphoid organs.