The chtl, chocolate-like, spontaneous mutation was discovered at The Jackson Laboratory in 1998 in the B6.129P2-Nos2tm1Lau/J (Stock # 002609) strain at generation N7F4+F16. These mice also carry the Nos2tm1Lau targeted mutation.
Read More +Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Nos2 | nitric oxide synthase 2, inducible |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | chtl | chocolate-like |
This strain is homozygous for Nos2tm1Lau.
This mutant has an overall lighter coat, tail and ears than a black +/+ control. Both males and females breed and it is maintained as a homozygous stock.
This chtl spontaneous mutation was discovered at The Jackson Laboratory in 1998 in the B6.129P2-Nos2tm1Lau/J (Stock # 002609) strain at generation N7F4+F16.
Allele Name | targeted mutation 1, Victor E Laubach |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | iNOS-; iNOS KO; iNOS-; NOS2-; NOS2tm/Lau; Nos2tm1Lau |
Gene Symbol and Name | Nos2, nitric oxide synthase 2, inducible |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 11 |
Molecular Note | A neomycin cassette replaced exons 12 and 13 of the gene, which encode the calmodulin-binding domain. Northern and Western blots of IFNg/LPS-stimulated peritoneal macrophages showed no detectable Nos2 mRNA or protein, respectively. |
Mutations Made By | Dr. Victor Laubach, University of Virginia Health Sci. Ctr. |
Allele Name | chocolate-like |
---|---|
Allele Type | Spontaneous |
Allele Synonym(s) | |
Gene Symbol and Name | chtl, chocolate-like |
Gene Synonym(s) | |
Strain of Origin | B6.129P2-Nos2tm1Lau/J |
Chromosome | 7 |
General Note | A test for allelism with homozygous cht mouse mated to a homozygous mutant mouse produced 32/32 progeny with a dull grey phenotype, unlike cht or a/a . These results suggested chtl is a new allele of the cht gene that produces a different phenotype from the original cht allele. When two matings of the grey color F1s from the allele test progeny were mated together, however, they produced segregating litters for a total of 10 cht looking mice and 3 normal black a/a mice. The appearance of the black mice means either the two mutations are pseudoalleles or modiefier genes can affect the phenotype expresssion. |
Molecular Note | The chocolate-like spontaneous mutation was found at The Jackson Laboratory in 1998. A test for allelism to the cht locus demonstrated that the two mutations are in pseudoalleles or that modifier genes in the two background strains can affect the phenotype expression. |
When using the Chocolate-like mouse strain in a publication, please cite the originating article(s) and include JAX stock #004684 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Nos2<tm1Lau>-chtl |
Frozen Mouse Embryo | B6.Cg-chtl Nos2<tm1Lau>/GrsrJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-chtl Nos2<tm1Lau>/GrsrJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-chtl Nos2<tm1Lau>/GrsrJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-chtl Nos2<tm1Lau>/GrsrJ Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.