NOD.Cg Prkdc^scid-Tg(CSF2)2Ygy Tg(IL3)1Ygy Tg(KITLG)3Ygy/YgyJ

Stock number: 004644
Product name: NOD.scid porcine IL-3-CSF2-KITLG
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools, Virology Research

Description

These NOD.scid porcine IL-3-CSF2-KITLG mice express a transgene encoding porcine interleukin 3 (IL3), porcine granulocyte macrophage-colony stimulating factor (CSF2), and soluble porcine stem cell factor (KITLG), and produce porcine IL-3, CSF2 and KITLG in bone marrow and spleen.

Detailed description

Tg(IL3)1Ygy, Tg(CSF2)2Ygy, and Tg(KITLG)3Ygy encode porcine interleukin 3 (IL3), Porcine granulocyte macrophage-colony stimulating factor (CSF2, commonly designated GM-CSF) and soluble Porcine stem cell factor (KITLG, commonly designated sSCF) respectively. All three are individually driven by the human cytomegalovirus promoter. These three transgenes were co-injected and they co-segregate. RT-PCR detects transgenic expression of porcine IL-3, CSF2 and KITLG in bone marrow and spleen in NOD.Cg Prkdc ^scid-Tg(IL3)1Ygy Tg(CSF2)2Ygy Tg(KITLG)3Ygy mice. Porcine IL3, CSF2, and KITLG are present in the serum of these mice in levels that can be detected by ELISA. NOD.Cg Prkdc ^scid-Tg(IL3)1Ygy Tg(CSF2)2Ygy Tg(KITLG)3Ygy/YgyJ not only provide a system in which long-term porcine tissue and stem cell engraftment can be achieved (Abe et al, 2002) but also is a useful tool for evaluating donor-specific tolerance induction by mixed chimerism across xenogeneic barriers (Chen et al, 2000). White blood cells of NOD.Cg Prkdc^scid-Tg(IL3)1Ygy Tg(CSF2)2Ygy Tg(KITLG)3Ygy mice are CD4^-, CD8^-, CD3^-, IgM^-, CD19^- and Mac-1⁺ by flow cytometric (FMC) analysis. White blood cells of Tg(IL3)1Ygy Tg(CSF2)2Ygy Tg(KITLG)3Ygy mice sub-lethally irradiated, i.v. injected with miniature swine bone marrow cells and i.p. injected with peripheral blood mononuclear cells (PBMC) are swine myeloid CD-2⁺, class II⁺ and CD16⁺ 20 weeks post bone marrow transplant by FMC. Non-transgenic littermates had low levels of chimerism detected at 3 weeks and non-existent at 7 weeks post bone marrow transplant (BMT). At 20 weeks post BMT these transgenic mice revealed high levels of chimerism in the spleen and bone marrow cells, versus no chimerism in any tissue of non-transgenic littermates. Treated transgenic mice showed peak levels of porcine T cell chimerism at 7 weeks post transplantation. Neither B nor T cells were detectable at 20 weeks post transplantation (Chen et al, 2000).

Allele

Symbol: Tg(KITLG)3Ygy
Name: transgene insertion 3, Yong-guang Yang
MGI accession ID: MGI:3057265
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(KITLG)3Ygy
Marker name: transgene insertion 3, Yong-guang Yang
Chromosome: UN
Strain of origin: NIH Swiss
Expressed genes: KITLG,KIT ligand,human
Molecular note: The transgene construct contains porcine kit ligand (KITL) controlled by the human cytomegalovirus promoter. The Tg(IL3)1Ygy, Tg(CSF2)2Ygy and Tg(KITLG)3Ygy transgenic constructs were co-injected into NIH/Swiss oocytes in David Bodine's lab at NCI.
General note: White blood cells from mice that carry this transgene in conjunction with Tg(IL3)1Ygy and Tg(CSF2)2Ygy and that are also homozygous for Prkdc^scid are CD4^-, CD8^-, CD3^-, IgM^-, CD19^- and Mac-1⁺ by flow cytometric (FMC) analysis.

Allele

Symbol: Tg(CSF2)2Ygy
Name: transgene insertion 2, Yong-guang Yang
MGI accession ID: MGI:3057263
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(CSF2)2Ygy
Marker name: transgene insertion 2, Yong-guang Yang
Chromosome: UN
Strain of origin: NIH Swiss
Expressed genes: CSF2,colony stimulating factor 2 (granulocyte-macrophage),pig, domestic
Molecular note: The transgene construct contains the porcine colony stimulating factor 2 (granulocyte-macrophage) sequence (CSF2) controlled by the human cytomegalovirus promoter. The Tg(IL3)1Ygy, Tg(CSF2)2Ygy and Tg(KITLG)3Ygy transgenic constructs were co-injected into NIH/Swiss oocytes in David Bodine's lab at NCI.
General note: White blood cells from mice that carry this transgene in conjunction with Tg(IL3)1Ygy and Tg(KITLG)3Ygy and that are also homozygous for Prkdc^scid are CD4^-, CD8^-, CD3^-, IgM^-, CD19^- and Mac-1⁺ by flow cytometric (FMC) analysis.

Allele

Symbol: Tg(IL3)1Ygy
Name: transgene insertion 1, Yong-guang Yang
MGI accession ID: MGI:3057194
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(IL3)1Ygy
Marker name: transgene insertion 1, Yong-guang Yang
Chromosome: UN
Strain of origin: NIH Swiss
Expressed genes: IL3,interleukin 3 (colony-stimulating factor, multiple),pig, domestic
Molecular note: The transgene construct contains the porcine interleukin 3 sequence controlled by the human cytomegalovirus promoter. The Tg(IL3)1Ygy, Tg(CSF2)2Ygy and Tg(KITLG)3Ygy transgenic constructs were co-injected into NIH/Swiss oocytes in David Bodine's lab at NCI.
General note: White blood cells from mice that carry this transgene in conjunction with Tg(CSF2)2Ygy and Tg(KITLG)3Ygy and that are also homozygous for Prkdc^scid are CD4^-, CD8^-, CD3^-, IgM^-, CD19^- and Mac-1⁺ by flow cytometric (FMC) analysis.

Allele

Symbol: Prkdc^scid
Name: severe combined immunodeficiency
MGI accession ID: MGI:1857113
Generation method: Spontaneous
Synonyms: scid
Marker symbol: Prkdc
Marker name: protein kinase, DNA activated, catalytic polypeptide
Marker synonyms: DNAPDcs; DNAPK; DNA-PK; DNAPKc; DNA-PKC; DNA-PKcs; DNPK1; DOXNPH; dxnph; HYRC; HYRC1; IMD26; p350; slip; XRCC7
Chromosome: 16
Strain of origin: C.BKa-Igh^b/Icr
Site of expression: T and B lymphocytes.
Molecular note: A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*).