Mice homozygous for the targeted allele are viable, normal in size and do not display any gross physical or behavioral abnormalities. No transcripts are detected in kidney, testis or bone marrow. Bone marrow cells derived from homozygous mice fail to display the synergistic growth reaction usually observed in response to the combined treatment with interleukin-11 and interleukin-3 or stem cell factor in hematopoietic assays. Homozygous females are infertile due to defective decidualization the process necessary for successful implantation whereby the uterine stroma transforms into a specialized tissue known as decidua. Fertilty of homozygous males is normal. This mutant mouse strain may be useful in studies related to hematopoiesis and female reproduction.

Development

A targeting vector containing neomycin phosphotransferase and thymidine kinase cassettes was used to disrupt parts of exons 8 and 13 and all of exons 7-12 of the targeted gene. The construct was electroporated into 129S1/Sv-p⁺ Tyr⁺ Kitl^Sl-J-derived W9.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Nandurkar HH; Robb L; Tarlinton D; Barnett L; Kontgen F; Begley CG. 1997. Adult mice with targeted mutation of the interleukin-11 receptor (IL11Ra) display normal hematopoiesis. Blood 90(6):2148-59PubMed: 9310465 MGI: J:42921

View All References

Genetics

Il11ra1^tm1Wehi

Allele Symbol: Il11ra1^tm1Wehi

Allele Name	targeted mutation 1, Walter and Eliza Hall Institute of Medical Research
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	Il11ra1^tm1Wehi; targeted mutation 1, Walter and Eliza Hall Institute of Medical Research
Gene Symbol and Name	Il11ra1, interleukin 11 receptor, alpha chain 1
Gene Synonym(s)	expressed sequence AI314697; CRSDA; Il11ra; AI314697; Il-11ra-alpha; Il11ra; NR1; interleukin 11 receptor, alpha chain; Il-11ra
Strain of Origin	129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Chromosome	4
Molecular Note	A neomycin selection cassette replaced a genomic fragment containing exons 9-12 and part of 8 and 13. Northern blot analysis on RNA derived from kidney and testis of homozygous mice demonstrated that no detectable transcript was produced from this allele.
Mutations Made By	Laura Savatski, Blood Research Institute

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Il11ra1^tm1Wehi related

Reproductive Biology Research
- Fertility Defects
  - females only

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Il11ra1^tm1Wehi/Il11ra1^tm1Wehi
involves: 129S1/Sv * C57BL/6

mammalian phenotype

digestive/alimentary phenotype
- Normal - adult homozygotes do NOT develop spontaneous gastric hyperplasia or gastric adenomas

hematopoietic system phenotype
- Normal - adult homozygotes exhibit normal steady-state hematopoiesis, with normal numbers of peripheral blood white blood cells, hematocrit, and platelets
- Normal - bone marrow and spleen contain normal numbers of cells of all hematopoietic lineages, including megakaryocytes
- Normal - in addition, homozygotes show normal kinetics of hematopoietic recovery in response to cytotoxic stress or phenylhydrazine-induced acute hemolysis

immune system phenotype
- Normal - adult homozygotes do NOT develop degenerative joint disease
- Normal - adult homozygotes show normal levels of Ig isotypes as well as a normal antibody response to a T-cell-dependent antigen (NP)

mortality/aging
- Normal - homozygotes are viable and appear phenotypically normal both at birth and during adulthood (at least up to 8 months of age)

reproductive system phenotype

female infertility

Genotype: Il11ra1^tm1Wehi/Il11ra1^tm1Wehi
either: (involves: 129S1/Sv) or (involves: 129S1/Sv * C57BL/6)

embryo phenotype

abnormal decidua basalis morphology
- the decidua basalis is entirely absent, being replaced by multiple layers of trophoblast giant cells

abnormal decidua capsularis morphology
- in a few cases, the decidua capsularis is present but significantly thinner

abnormal decidualization
- at 4.5 dpc, uteri of pregnant homozygotes display a reduced secondary decidual response to the implanting blastocyst
- at 5.5-7.5 dpc, mutant deciduae are significantly smaller, displaying hemorrhage in the uterine lumen, disruption of the antimesometrium, and overgrowth of giant trophoblast cells in the mesometrial portion of the deciduum
- notably, pseudopregnant homozygotes show reduced deciduoma formation in response to an artificial (oil) stimulus, albeit with partial preservation of mesometrial decidual tissue

abnormal placenta development
- is present but significantly thinner
- in a few cases, embryos surviving in 9.5 dpc mutant uteri display fetal components of the chorioallantoic placenta; however, the decidua basalis is entirely absent, being replaced by multiple layers of trophoblast giant cells, while the decidua capsularis is present but significantly thinner
- in most cases, embryos surviving in 9.5 dpc mutant uteri contain fibrinoid material and inflammatory cells in the space normally occupied by the placenta

increased trophoblast giant cell number
- pregnant homozygotes exhibit a significantly increased number of secondary trophoblast giant cells in the mutant decidua

mammalian phenotype

reproductive system phenotype
- Normal - virgin female homozygotes display normal estrous cyclicity and mating behavior, as well as histologically normal ovaries, oviducts, vagina and uterus

mortality/aging

embryonic lethality during organogenesis, complete penetrance
- however, embryos developing in homozygous mutant mothers appear necrotic at >7.5 dpc, with rare instances (<1%) of intact embryos detected at 9.5 dpc, and no live embryos found after 10.5 dpc
- intercrosses of heterozygotes produce viable homozygous offspring at the expected Mendelian ratios
- notably, 2.5 dpc mutant embryos survive normally when transferred in foster uteri, whereas wild-type embryos fail to do so in uteri of mutant mice

reproductive system phenotype

abnormal decidua basalis morphology
- the decidua basalis is entirely absent, being replaced by multiple layers of trophoblast giant cells

abnormal decidua capsularis morphology
- in a few cases, the decidua capsularis is present but significantly thinner

abnormal decidualization
- at 4.5 dpc, uteri of pregnant homozygotes display a reduced secondary decidual response to the implanting blastocyst
- at 5.5-7.5 dpc, mutant deciduae are significantly smaller, displaying hemorrhage in the uterine lumen, disruption of the antimesometrium, and overgrowth of giant trophoblast cells in the mesometrial portion of the deciduum
- notably, pseudopregnant homozygotes show reduced deciduoma formation in response to an artificial (oil) stimulus, albeit with partial preservation of mesometrial decidual tissue

impaired embryo implantation
- at 4.5-7.5 dpc, homozygotes display a normal number of implantation sites relative to wild-type mice
- however, mutants show reduced capillary permeability and blood flow in the uterine vascular bed at the site of blastocyst apposition

References

Nandurkar HH; Robb L; Tarlinton D; Barnett L; Kontgen F; Begley CG. 1997. Adult mice with targeted mutation of the interleukin-11 receptor (IL11Ra) display normal hematopoiesis. Blood 90(6):2148-59PubMed: 9310465 MGI: J:42921

Additional References

Robb L; Li R; Hartley L; Nandurkar HH; Koentgen F; Begley CG. 1998. Infertility in female mice lacking the receptor for interleukin 11 is due to a defective uterine response to implantation. Nat Med 4(3):303-8PubMed: 9500603 MGI: J:46263

Additional - Il11ra1^tm1Wehi related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR: Il11ra1^tm1Wehi
- Melt Curve Analysis: Il11ra1^tm1Wehi
- Genotyping resources and troubleshooting
Breeding Considerations

Chimeric animals were crossed to C57BL/6 mice by the originating investigator, Dr. Glenn Begley. Subsequently, the mice were backcrossed to C57BL/6 for twelve generations by the Blood Research Institute (Milwaukee) before arriving at The Jackson Laboratory. When maintained in a live colony, these mice were maintained by crossing heterozygous males to wildtype C57BL/6 females and reciprocal. Please note that homozygous females are infertile (see phenotype).
- Additional Breeding and Husbandry Support
Citation
When using the IL11Ra- mouse strain in a publication, please cite the originating article(s) and include JAX stock #004621 in your Materials and Methods section.

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Also Known As: IL11Ra-

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Il11ra1tm1Wehi

Allele Symbol: Il11ra1tm1Wehi

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Il11ra1tm1Wehi related

Mammalian Phenotype Terms by Genotype

Genotype: Il11ra1tm1Wehi/Il11ra1tm1Wehiinvolves: 129S1/Sv * C57BL/6

mammalian phenotype

reproductive system phenotype

Genotype: Il11ra1tm1Wehi/Il11ra1tm1Wehieither: (involves: 129S1/Sv) or (involves: 129S1/Sv * C57BL/6)

embryo phenotype

mammalian phenotype

mortality/aging

reproductive system phenotype

References

Additional References

Additional - Il11ra1tm1Wehi related

Genotyping Protocols

Breeding Considerations

Citation

Strain Interest Registration

Contact Information

Interest

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Il11ra1^tm1Wehi

Allele Symbol: Il11ra1^tm1Wehi

Genotype: Il11ra1^tm1Wehi related

Genotype: Il11ra1^tm1Wehi/Il11ra1^tm1Wehi
involves: 129S1/Sv * C57BL/6

Genotype: Il11ra1^tm1Wehi/Il11ra1^tm1Wehi
either: (involves: 129S1/Sv) or (involves: 129S1/Sv * C57BL/6)

Additional - Il11ra1^tm1Wehi related