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These Il11ra1 knock-out mice display normal steady-state hematopoiesis.
John W. Adamson, Blood Research Institute
Mice homozygous for the targeted allele are viable, normal in size and do not display any gross physical or behavioral abnormalities. No transcripts are detected in kidney, testis or bone marrow. Bone marrow cells derived from homozygous mice fail to display the synergistic growth reaction usually observed in response to the combined treatment with interleukin-11 and interleukin-3 or stem cell factor in hematopoietic assays. Homozygous females are infertile due to defective decidualization the process necessary for successful implantation whereby the uterine stroma transforms into a specialized tissue known as decidua. Fertilty of homozygous males is normal. This mutant mouse strain may be useful in studies related to hematopoiesis and female reproduction.
A targeting vector containing neomycin phosphotransferase and thymidine kinase cassettes was used to disrupt parts of exons 8 and 13 and all of exons 7-12 of the targeted gene. The construct was electroporated into 129S1/Sv-p+ Tyr+ KitlSl-J-derived W9.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts.
|Allele Name||targeted mutation 1, Walter and Eliza Hall Institute of Medical Research|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Il11ra1tm1Wehi; targeted mutation 1, Walter and Eliza Hall Institute of Medical Research|
|Gene Symbol and Name||Il11ra1, interleukin 11 receptor, alpha chain 1|
|Gene Synonym(s)||expressed sequence AI314697; CRSDA; Il11ra; AI314697; Il-11ra-alpha; Il11ra; NR1; interleukin 11 receptor, alpha chain; Il-11ra|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||A neomycin selection cassette replaced a genomic fragment containing exons 9-12 and part of 8 and 13. Northern blot analysis on RNA derived from kidney and testis of homozygous mice demonstrated that no detectable transcript was produced from this allele.|
|Mutations Made By|| |
Laura Savatski, Blood Research Institute
Chimeric animals were crossed to C57BL/6 mice by the originating investigator, Dr. Glenn Begley. Subsequently, the mice were backcrossed to C57BL/6 for twelve generations by the Blood Research Institute (Milwaukee) before arriving at The Jackson Laboratory. When maintained in a live colony, these mice were maintained by crossing heterozygous males to wildtype C57BL/6 females and reciprocal. Please note that homozygous females are infertile (see phenotype).
When using the IL11Ra- mouse strain in a publication, please cite the originating article(s) and include JAX stock #004621 in your Materials and Methods section.
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