Overview

Also Known As:NOD.scid AB0^nullDQ8

This strain which is MHC II deficient and has no functional T or B cells expresses a humanized transgenic HLA-DQ8 molecule that has been linked to IDDM development. This model is a valuable genetic tool for identifying the role of HLA-DQ8 in Type 1 Diabetes.

Donating Investigator

Dr. Leonard D. Shultz, The Jackson Laboratory

Genetic overview

Genetic Background	Generation
001289 NOD/ShiLt

Prkdc^scid

Allele Type	Gene Symbol	Gene Name
Spontaneous	Prkdc	protein kinase, DNA activated, catalytic polypeptide

H2-Ab1^tm1Doi

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	H2-Ab1	histocompatibility 2, class II antigen A, beta 1

Tg(HLA-DQA1,HLA-DQB1)1Dv

Allele Type
Transgenic (Inserted expressed sequence, Humanized sequence)

View Genetics

Research Applications

Research Tools
Internal/Organ Research
Immunology, Inflammation and Autoimmunity Research
Virology Research
Diabetes and Obesity Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

This strain which is MHC II deficient and has no functional T or B cells expresses a humanized transgenic HLA-DQ8 molecule that has been linked to IDDM development. This model is a valuable genetic tool for identifying the role of HLA-DQ8 in Type 1 Diabetes.

Development

A 30kb DNA fragment containing the DQA*0301 gene from cosmid H11A and a 38kb DNA fragment containing the DQB*0302 gene from cosmid X10A were microinjected into (CBA/J x B10.M)F2 embryos. To establish germline transmission founders were mated to the B10.M strain. This transgene was then mated to (B6x129)-H2-Ab1 targeted mutation. The transgene positive, H2-Ab1 mice were intercrossed to make homozygote for the H2-Ab1 targeted allele (Nabozny et al, 1996). Flynn et al, 2002, backcrossed the transgenic HLA-DQ8, H2-Ab1 mice to NOD for 10 generations. Schultz et al (Unpublished), backcrossed the NOD-Tg(HLA-DQ8)1Sz H2-Ab1^tm1Doi to NOD.Cg-Prkdc^scid H2-Ab1^tm1Doi/Sz. In 2003, the Type 1 Diabetes Resource received NOD.Cg-Prkdc^scidTg(HLA-DQ8)1Sz H2-Ab1^tm1Doi/Sz at [NE10F?+4p]+F1.

Expression Data

Expressed Gene	HLA-DQA1, major histocompatibility complex, class II, DQ alpha 1, human
Expressed Gene	HLA-DQB1, major histocompatibility complex, class II, DQ beta 1, human
Site of Expression

Control Suggestions

Prkdc^scid/Prkdc^scid, H2-Ab1/H2-Ab1, +/+
Noncarrier

Additional Information

Considerations for Choosing Controls

Genetics

Prkdc^scid

Allele Symbol: Prkdc^scid

Allele Name	severe combined immunodeficiency
Allele Type	Spontaneous
Allele Synonym(s)	SCID
Gene Symbol and Name	Prkdc, protein kinase, DNA activated, catalytic polypeptide
Gene Synonym(s)
Site of Expression	T and B lymphocytes.
Strain of Origin	C.BKa-Igh^b/Icr
Chromosome	16
Molecular Note	A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*).

H2-Ab1^tm1Doi

Allele Symbol: H2-Ab1^tm1Doi

Allele Name	targeted mutation 1, Christophe Benoist and Diane Mathis
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	A beta⁰; A^bbeta^-; AB0; Abeta-; Class II⁰; H2-Ab1^tm1Dim; H2-Ab1tm1Doi; II⁰; MHC class II-; MHCII^-; MHC-II-k.o.
Gene Symbol and Name	H2-Ab1, histocompatibility 2, class II antigen A, beta 1
Gene Synonym(s)
Strain of Origin	129S2/SvPas
Chromosome	17
Molecular Note	The second exon was disrupted by the insertion of a neomycin resistance gene. In addition, the ES cell line used was derived from the 129S2/SvPas strain, which carries a deletion in the promoter region of H2-Ea. Consequently, these MHC class II molecule-deficient mice lacked cell surface expression of both class II-A and class II-E MHC proteins.
Mutations Made By	Christophe Benoist, Joslin Diabetes Center

Tg(HLA-DQA1,HLA-DQB1)1Dv

Allele Symbol: Tg(HLA-DQA1,HLA-DQB1)1Dv

Allele Name	transgene insertion 1, Chella David
Allele Type	Transgenic (Inserted expressed sequence, Humanized sequence)
Allele Synonym(s)	Abeta⁰.DQ8; DQ8; HLA-DQ8; HLA-DQ8 Tg; HLA-DQA10301/DQB10302; Tg(HLA-DQA1301,HLA-DQB1302)1Dv
Gene Symbol and Name	Tg(HLA-DQA1,HLA-DQB1)1Dv, transgene insertion 1, Chella David
Gene Synonym(s)
Promoter	HLA-DQA1, major histocompatibility complex, class II, DQ alpha 1, human
Promoter	HLA-DQB1, major histocompatibility complex, class II, DQ beta 1, human
Expressed Gene	HLA-DQA1, major histocompatibility complex, class II, DQ alpha 1, human
Expressed Gene	HLA-DQB1, major histocompatibility complex, class II, DQ beta 1, human
Strain of Origin	(CBA/J x B10.M-H2^f)F2
Chromosome	UN
General Note	Flow cytometric analysis of peripheral blood leukocytes (PBL) using a monoclonal antibody to HLA-DQ demonstrated that transgenic mice express the transgenes. 25-40% of the PBLs of mixed-background transgenic mice homozygous for the targeted MHC class II mutation H2-Ab1^tm1Dim express the transgenes. Lymph nodes of such mice contain three-fold the levels of CD4⁺ CD44⁺ T-cells as do those of nontransgenic MHC class II-deficient mice, levels similar to those of class II-sufficient mice. Immunization of transgenic H2-Ab1^tm1Dim homozygous mice with bovine collagen II results in a strong IgG antibody response to the immunogen, and 66% of these mice develop severe, persistent arthritis. (J:88296)
Molecular Note	A 30-kb human genomic DNA fragment containing the entire HLA-DQA10301 gene and a promoter-truncated HLA-DQB10302 gene from cosmid H11A and a 38-kb DNA fragment containing the entire HLA-DQB1*0302 gene from cosmid X10A were coinjected.
Mutations Made By	Chella David, Mayo Clinic

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Prkdc^scid related

Research Tools
- Toxicology Research
  - xenograft/transplant host
- Cancer Research
  - B and T cell deficiency, xenograft/transplant host
Internal/Organ Research
- Lymphoid Tissue Defects
  - B and T cell deficiency
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - B and T cell deficiency
Virology Research
- B and T Cell Deficiency
  - AIDS research tool

Diabetes and Obesity Research
- Type 1 Diabetes (IDDM)
- Type 1 Diabetes (IDDM) Analysis Strains
  - NOD Congenics with Mutations Affecting Immunocompetence
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - MHC class II defects
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - MHC class II deficient
- HLA transgenics

Mammalian Phenotype Terms by Genotype

References

Additional - H2-Ab1^tm1Doi related

Additional - Prkdc^scid related

Additional - Tg(HLA-DQA1,HLA-DQB1)1Dv related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- End Point Analysis:Prkdc End Point
- Separated PCR:H2-Ab1/H2-Ab1
- Standard PCR:Tg(HLA-DQA1)
- Standard PCR:Tg(HLA-DQB1)
- Genotyping resources and troubleshooting
Breeding Considerations

These mice are immunodeficient and need to be maintained in a high barrier environment with sterile food and water or given antibiotic in the water.
Embryos frozen are either Prkdc^scid/Prkdc^scid, Tg/+, H2-Ab1^tm1Doi/H2-Ab1^tm1Doi or Prkdc^scid/Prkdc^scid, +/+, H2-Ab1^tm1Doi/H2-Ab1^tm1Doi
Recommended breeding scheme: Prkdc^scid/Prkdc^scid, Tg/+, H2-Ab1^tm1Doi/H2-Ab1^tm1Doi X Prkdc^scid/Prkdc^scid, +/+, H2-Ab1^tm1Doi/H2-Ab1^tm1Doi and reciprocal.
Affected mutant: Prkdc^scid/Prkdc^scid, Tg/+, H2-Ab1^tm1Doi/H2-Ab1^tm1Doi.
- Additional Breeding and Husbandry Support
Appearance
- albino, pink eyed
  Related Genotype: A/A Tyr^c/Tyr^c
Citation
When using the NOD.scid AB0^nullDQ8 mouse strain in a publication, please cite the originating article(s) and include JAX stock #004606 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Not-For-Profit & Academic Pricing

Service/Product	Description	Price
	Homozygous for Prkdc<scid>, Homozygous for H2-Ab1<tm1Doi> , Hemizygous or Homozygous(Tg/?) or no-carrier for Tg(HLA-DQA1,HLA-DQB1)1Dv

Related Products and Services

Frozen Mouse Embryo	NOD.Cg-Prkdc<scid> H2-Ab1<tm1Doi> Tg(HLA-DQA1 HLA-DQB1)1Dv/S	$2595.00
Frozen Mouse Embryo	NOD.Cg-Prkdc<scid> H2-Ab1<tm1Doi> Tg(HLA-DQA1 HLA-DQB1)1Dv/S	$2595.00
Frozen Mouse Embryo	NOD.Cg-Prkdc<scid> H2-Ab1<tm1Doi> Tg(HLA-DQA1 HLA-DQB1)1Dv/S	$3373.50
Frozen Mouse Embryo	NOD.Cg-Prkdc<scid> H2-Ab1<tm1Doi> Tg(HLA-DQA1 HLA-DQB1)1Dv/S	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Not Available to Companies or for Commercial Use

Use of MICE only available to non-profit entities.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:NOD.scid AB0nullDQ8

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Prkdcscid

Allele Symbol: Prkdcscid

H2-Ab1tm1Doi

Allele Symbol: H2-Ab1tm1Doi

Tg(HLA-DQA1,HLA-DQB1)1Dv

Allele Symbol: Tg(HLA-DQA1,HLA-DQB1)1Dv

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Prkdcscid related

Mammalian Phenotype Terms by Genotype

References

Additional - H2-Ab1tm1Doi related

Additional - Prkdcscid related

Additional - Tg(HLA-DQA1,HLA-DQB1)1Dv related

Genotyping Protocols

Breeding Considerations

Appearance

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Not-For-Profit & Academic Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Also Known As:NOD.scid AB0^nullDQ8

Prkdc^scid

Allele Symbol: Prkdc^scid

H2-Ab1^tm1Doi

Allele Symbol: H2-Ab1^tm1Doi

Genotype: Prkdc^scid related

Additional - H2-Ab1^tm1Doi related

Additional - Prkdc^scid related