These Tg NOD.RIP-rTA mice express a transgene containing the reverse tetracycline regulatable transactivator (rtTA) expressed in the pancreatic beta cells.
Christophe Benoist, Joslin Diabetes Center
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Transactivator) |
Tg(Ins2-rtTA)2Doi encodes the reverse tetracycline regulatable transactivator (rtTA) regulated by the rat insulin promoter (Ins2, commonly designated RIP), and is expressed in the pancreatic beta cells. Mice hemizygous for this transgenic insert are viable, fertile, normal in size, display normal NOD diabetes onset, but do not display any other gross physical or behavioral abnormalities. When transgenic are mated to a second transgenic strain carrying a target gene regulated by the tetO responsive elements, expression of the target gene is turned on by the tetracycline analog, doxycycline (dox). Dox can be administered orally in the food or water. This strain provides a Tet-On tool that permits the inducible expression of genes in the pancreatic beta cells during various stages of diabetes development (As reported by donating investigator, data unpublished).
Christophe Benoist et al., injected the Ins2-rtTA transgenic construct into (C57BL/6J x SJL)F1 oocytes and backcrossed the resulting Tg(Ins2-rtTA)2Doi allele onto the NOD background for 18 generations. In 2003, N18 mice were backcrossed once to NOD/ShiLtJ at The Jackson Laboratory, and are maintained Tg/? x Tg/?
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
---|---|
Site of Expression | Expresses rtTA in pancreas. |
Allele Name | transgene insertion 2, Christophe Benoist |
---|---|
Allele Type | Transgenic (Transactivator) |
Allele Synonym(s) | RIP-rtTA |
Gene Symbol and Name | Tg(Ins2-rtTA)2Doi, transgene insertion 2, Christophe Benoist |
Gene Synonym(s) | |
Promoter | Ins2, insulin 2, rat |
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
Site of Expression | Expresses rtTA in pancreas. |
Strain of Origin | (C57BL/6J x SJL)F1 |
Chromosome | UN |
Molecular Note | The transgene encodes the reverse tetracycline regulatable transactivator (rtTA) regulated by the rat insulin promoter (Ins2). rtTA is expressed in pancreatic beta cells. |
Mutations Made By | Christophe Benoist, Joslin Diabetes Center |
Footpad injections of Complete Freunds Adjuvent (CFA) will delay diabetes onset, thus extending the lifespan of breeders.
When using the NOD.RIP-rTA mouse strain in a publication, please cite the originating article(s) and include JAX stock #004602 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier or Homozygous for Tg(Ins2-rtTA)2Doi/, 1 pair minimum |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.