NOD.Cg-Tg(Ins2-rtTA)2Doi/DoiJ

Stock number: 004602
Product name: NOD.RIP-rTA
Research areas: Diabetes and Obesity Research, Research Tools

Description

These Tg NOD.RIP-rTA mice express a transgene containing the reverse tetracycline regulatable transactivator (rtTA) expressed in the pancreatic beta cells.

Detailed description

Tg(Ins2-rtTA)2Doi encodes the reverse tetracycline regulatable transactivator (rtTA) regulated by the rat insulin promoter (Ins2, commonly designated RIP), and is expressed in the pancreatic beta cells. Mice hemizygous for this transgenic insert are viable, fertile, normal in size, display normal NOD diabetes onset, but do not display any other gross physical or behavioral abnormalities. When transgenic are mated to a second transgenic strain carrying a target gene regulated by the tetO responsive elements, expression of the target gene is turned on by the tetracycline analog, doxycycline (dox). Dox can be administered orally in the food or water. This strain provides a Tet-On tool that permits the inducible expression of genes in the pancreatic beta cells during various stages of diabetes development (As reported by donating investigator, data unpublished).

Development

Christophe Benoist et al., injected the Ins2-rtTA transgenic construct into (C57BL/6J x SJL)F1 oocytes and backcrossed the resulting Tg(Ins2-rtTA)2Doi allele onto the NOD background for 18 generations. In 2003, N18 mice were backcrossed once to NOD/ShiLtJ at The Jackson Laboratory, and are maintained Tg/? x Tg/?

Allele

Symbol: Tg(Ins2-rtTA)2Doi
Name: transgene insertion 2, Christophe Benoist
MGI accession ID: MGI:3763728
Generation method: Transgenic
Attributes: Transactivator
Marker symbol: Tg(Ins2-rtTA)2Doi
Marker name: transgene insertion 2, Christophe Benoist
Chromosome: UN
Strain of origin: (C57BL/6J x SJL)F1
Expressed genes: rtTA,reverse tetracycline-controlled transactivator,E. coli
Site of expression: Expresses rtTA in pancreas.
Molecular note: The transgene encodes the reverse tetracycline regulatable transactivator (rtTA) regulated by the rat insulin promoter (Ins2). rtTA is expressed in pancreatic beta cells.