A C57BL/6J congenic version of this strain is available as Stock No. 006440.
When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed Pten allele, and for example may be useful in studies of neurogenesis, glia differentiation and cerebellar development.
Hong Wu, UCLA
These mice possess loxP sites flanking exon 5 of the targeted gene. Mice homozygous for the "floxed" allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.
For example, when crossed to a strain expressing Cre recombinase in astrocytes (see Stock No. 012887), this mutant mouse strain may be useful in studies of neurogenesis.
When crossed to a strain expressing Cre recombinase in the central nervous system (see Stock No. 004600), this mutant mouse strain may be useful in studies of glia differentiation and cerebellar development.
A loxP site flanked targeting vector containing hygromycin resistance and thymidine kinase genes was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 5 of the targeted gene, and another loxP site was inserted upstream of exon 5. This construct was electroporated into 129S4/SvJae derived LW-1 embryonic stem (ES) cells, which were transiently transfected with a Cre-recombinase vector to remove the selection cassette. Correctly targeted ES cells were injected into BALB/c blastocysts. The resulting chimeric animals were crossed to BALB/cAnNTac mice before being made homozygous.
|Allele Name||targeted mutation 1, Hong Wu|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Ptenex5lox; PTENF; Ptenfl; Ptenflox; Ptenflx; Ptenfx; Ptenlox; Ptenloxp|
|Gene Symbol and Name||Pten, phosphatase and tensin homolog|
|Site of Expression||Pten is a widely expressed tumor suppresser gene.|
|Strain of Origin||129S4/SvJae|
|General Note||Phenotypic Similarity to Human Syndrome: PTEN Hamartoma Tumor Syndrome (see OMIM:601728 Phosphatase And Tensin Homolog; PTEN) J:199362|
|Molecular Note||A loxP flanked hygromycin resistance cassette was inserted 5' to exon 5, and a single loxP site was inserted 3' to exon 5, which encodes the phosphatase domain. The hygromycin cassette was removed in ES cells by transient Cre recombinase expression prior to the production of chimeric mice, leaving a single loxP site in place of the cassette. These insertions do not appear to have any effect on the normal function of the gene.|
|Mutations Made By|| |
Hong Wu, UCLA
When maintaining a live colony, homozygous mice are bred.
When using the Ptenflox mouse strain in a publication, please cite the originating article(s) and include JAX stock #004597 in your Materials and Methods section.